User Manual

Predefined methods
Eppendorf
®
PlateReader AF2200
English (EN)
62
User defined method parameters
• Using the predefined UV/Vis filter slide the measurement is performed at 600 nm with default
measurement parameters.
• Define the number of blank replicates (possible number: 1 – 8).
• Define the number of standards (possible number: 2 – 12).
• Define number of standard replicates (possible number: 1 – 8).
• Define the concentrations of each standard (Press triangle button to open the menu).
• Define the number of samples (max. 93, depends on number of blanks and standards).
• Define the unit of your concentration.
• Check if entered parameters are correct.
• Required plate layout: Pipette in a vertical order, starting with blanks at position A1. Standards are
pipetted in subsequent wells, samples must be placed in the following wells.
• Press Start.
• For method results and data evaluation principle see chapter method results and evaluation procedure.
Plate layout
Abb. 6-5: Metho d strip: Protein quantification (BCA 562 (600) nm with standards)
Fig. 6-5: Method strip: Protein quantification (BCA 562 (600) nm with standards)
The selected number of blanks, standards and samples are shown in the plate view.