5404 900.
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You will find a detailed description of these figures in your language in Chapters 1.1/2.1 and 4.1/5.1. Abb. 1: Front and rear view of the Centrifuge 5424 1 2 1 2 5 3 6 4 7 8 9 Fig. 1: Front and rear view of the Centrifuge 5424 Abb. 2: Front and rear view of the Centrifuge 5424 R 1 2 1 2 5 3 4 7 9 8 6 10 11 Fig.
Abb. 3: Control panel of the Centrifuge 5424 / 5424 R Fig. 3: Control panel of the Centrifuge 5424 / 5424 R 1 Call and select the menu parameters 2 Switch the centrifuging speed displayed (rpm/rcf) 3 Display 4 Short Spin centrifugation 5 Release lid 6 Start and stop centrifugation 7 Set the speed of centrifugation Dependent on device version designed as a key or dial.
Centrifuge 5424 / 5424 R — Operating manual Table of contents Table of contents 1 3 4 5 EN User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.2 Warning signs and hazard icons. . . . . . . . . . . . . . . . . . . . .
Centrifuge 5424 / 5424 R — Operating manual Table of contents EN Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1 Prepare cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2 Perform cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Centrifuge 5424 / 5424 R — Operating manual 1 User instructions 1 1.1 Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R User instructions EN Using this manual Operating manual Please read this operating manual completely before using the device for the first time. Please view this operating manual as part of the product and keep it somewhere easily accessible. When passing the device on to third parties, be sure to include this operating manual.
Centrifuge 5424 / 5424 R — Operating manual 2 Product description 2 EN Product description 2.1 Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R Main illustration The depiction of the Centrifuge 5424 / 5424 R can also be found on the front fold-out page (see Fig. 1 and Fig. 2). Operating manual Abb. 1: Front and rear view of the Centrifuge 5424 1 2 1 2 5 3 6 4 7 8 9 Fig. 1: Front and rear view of the Centrifuge 5424 Abb.
Centrifuge 5424 / 5424 R — Operating manual 2 Product description 2.2 2.2.1 EN Delivery package Centrifuge 5424 Order No. Description (North America) 1 - - Centrifuge 5424 See chapter Ordering Information for corresponding device version, equipment and order number 1 or 5424 852.122 5424 852.130 950004266 950004240 Fuses 3.15 AT for 230 V, 2 pieces 6.3 AT for 120 V/100 V, 2 pieces 1 5416 301.001 022634305 Rotor key Standard 1 5703 350.
Centrifuge 5424 / 5424 R — Operating manual 2 Product description EN 2.4 Rotors Operating manual You can operate the Centrifuge 5424 / 5424 R with the following rotors. Before use of sample tubes, please note the manufacturer's specifications with regard to centrifugation resistance (max. rcf). Max. capacity Max. g-force (rcf) / speed (rpm) without adapter (5424: 120 V, 5424 R) Max. g-force (rcf) or Notes speed (rpm) without adapter (5424: 230 V/100 V) Max.
Centrifuge 5424 / 5424 R — Operating manual 2 Product description 2.4.1 EN Rcf display and calculation Rotor Adapters Max. centrifugation radius rmax [cm] Max. g-force (rcf) (5424 120 V, 5424 R) Max. g-force (rcf) (5424 230 V/100 V) FA-45-24-11, without adapter 8.4 21,130 20,238 FA-45-24-11-Special 0.2 mL 6.3 15,848 15,179 0.4 mL 8.4 21,130 20,238 0.5 mL 7.3 18,363 17,558 0.6 mL 8.4 21,130 20,238 without adapter 7.2 18,111 17,347 0.2 mL 5.1 12,829 12,288 0.4 mL 7.
Centrifuge 5424 / 5424 R — Operating manual 3 Safety 3 EN 3.1 Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R Safety Intended use Operating manual The Centrifuge 5424 / 5424 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes. Poor safety due to incorrect accessories and spare parts.
Centrifuge 5424 / 5424 R — Operating manual 3 Safety EN Operating manual For the other rotors and rotor lids of this centrifuge (see Rotors on p. 10) there is no limit for their service life, as long as the following conditions are met: proper use, recommended maintenance and undamaged condition. Lids of aerosol-tight rotors must be replaced after 50 autoclave cycles to ensure aerosol tightness.
Centrifuge 5424 / 5424 R — Operating manual 3 Safety EN Damage to health when handling infectious liquids and pathogenic germs. Observe the national regulations for handling these substances, the biological security level Operating manual of your laboratory, the material safety data sheets and the manufacturer's application notes. WARNING! Use suitable aerosol-tight closure systems for the centrifugation of these substances.
Centrifuge 5424 / 5424 R — Operating manual 3 Safety EN Risk of injury from asymmetric loading of rotors. Load rotors symmetrically with identical tubes. Only load adapters with suitable tubes. Operating manual CAUTION! Always use tubes of the same type (weight, material/density and volume). Check symmetric loading by balancing the adapters and tubes used with scales. Risk of injury from overloaded rotor. The Centrifuge 5424 / 5424 R is designed for the centrifugation of material with a max.
Centrifuge 5424 / 5424 R — Operating manual 3 Safety EN Sample tubes heat up. Operating manual In uncooled centrifuges the temperature in the rotor chamber, rotor and sample can rise to above 40°C dependent on the run time, g-force (rcf) or speed (rpm) and ambient temperature. NOTICE! 3.5.5 Note that this can reduce the centrifugation resistance of the sample tubes. Please note the temperature resistance of the samples.
Centrifuge 5424 / 5424 R — Operating manual 4 Installation 4 4.1 Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R Installation EN Selecting the location If a fault occurs, objects in the immediate vicinity of the devices could get damaged. Operating manual In accordance with the recommendations of EN 61010-2-020, leave a safety distance of 30 cm clear around the device during operation. NOTICE! Damage from overheating.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation 5 EN Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R Operation 5.1 Overview of operating controls Operating manual The Centrifuge 5424 / 5424 R is available in two versions: keypad or rotary knobs. This operating manual generally describes how to operate the keypad version. However, it also applies to the rotary knob version.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation EN Display of the Centrifuge 5424 / 5424 R Fig. 4: Display of the Centrifuge 5424 R. The display of the Centrifuge 5424 is similar. 1 Centrifuging duration 2 Operating manual Abb. 4: Status of the key lock (LOCK) : Centrifuging parameters cannot be modified unintentionally. : No key lock. 3 Status of the function ATSET : Start of operation when reaching 95% of the preset g-force (rcf) or speed (rpm).
Centrifuge 5424 / 5424 R — Operating manual 5 Operation EN 5.2 Device menu Operating manual Tab. 1: Menu structure of the Centrifuge 5424 / 5424 R. All menu levels contain the additional menu item Back. Level 1 (M I) Level 2 Function (M 2) SOFT On Rotor accelerates and brakes slowly. Soft ramp: Reduce speed of acceleration and braking ramp. Not used for short spin centrifugation. Off Rotor accelerates and brakes rapidly.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation 5.3 EN Menu navigation To change settings in the device menu, proceed as follows: Open the menu. 2. Select the desired menu item. 3. Confirm your selection. 4. Select the setting of the parameters in question. Operating manual 1. Confirm the changed setting. 5. The BACK menu item belonging to the first menu level appears. 6. Exit the menu.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation EN Risk from damaged or overloaded tubes! When loading the rotor, note the safety instructions with regard to hazards from overloaded or Operating manual damaged tubes(see Warnings for intended use on p. 13) CAUTION! Rotor lid! • Fixed-angle rotors may only be operated with the appropriate rotor lid in each case. This is clearly shown by the identical rotor name labeling on the rotor and on the rotor lid.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation 5.4.4 EN Close centrifuge lid Centrifuge lid can crush. Keep hands clear. Operating manual When opening or closing the device lid, do not reach between the lid and device or into the latching mechanism of the lid. WARNING! Always open the centrifuge lid completely to prevent it from falling. 1. Check correct attachment of rotor and rotor lid. 2. Push down the centrifuge lid until the lid latch engages and the lid is automatically closed.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation EN 2. Press the start/stop key to terminate the temperature control run early. Operating manual After the temperature control run is complete the centrifuge keeps the rotor chamber, with the centrifuge lid closed, at the set temperature, if the temperature is below the ambient temperature. Irrespective of the target temperature, however, this continuous cooling does not go below 4°C to prevent the rotor chamber from freezing. 5.5.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation 5.6.1 EN Centrifuging with time preset Perform the following steps in the sequence described. Operating manual 1. Use time to set the run time. 2. Only 5424 R: Use temp to set the temperature. 3. Use speed to set the g-force (rcf) or speed (rpm). 4. Press start/stop to start centrifuging. During centrifugation • In the display flashes while the rotor is running. • Only 5424 R: The actual temperature is displayed.
Centrifuge 5424 / 5424 R — Operating manual 5 Operation EN 5.6.3 Short spin centrifugation Operating manual You can carry out a short spin run with the currently set or with the maximum g-force (rcf) or speed (rpm) of the rotor used. This is set in the device menu (see Device menu on p. 20) before executing the following steps in the sequence specified: Performing short spin centrifugation 1. A short run at current g-force (rcf) or speed (rpm) can be set directly using the speed arrow keys. 2.
Centrifuge 5424 / 5424 R — Operating manual 6 Maintenance 6 6.1 Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R Maintenance EN Prepare cleaning / disinfection Clean at least once a week and clean if the accessible surfaces of the device and its accessories are acutely contaminated. Operating manual Clean the rotor regularly. This way the rotor is protected and the durability is prolonged. Furthermore, observe the notes on decontamination (see Decontamination before shipping on p.
Centrifuge 5424 / 5424 R — Operating manual 6 Maintenance EN Damage from UV and other high-energy radiation. Do not disinfect by any method based on UV, beta and gamma radiation or other high-energy Operating manual radiation. NOTICE! Autoclave All Rotors, rotor lid and adapter can be autoclaved (121°C, 20 min). Hint! After a maximum of 50 autoclave cycles, replace the lids of the aerosol-tight rotors. Aerosol-tightness Check that the seals are intact before use.
Centrifuge 5424 / 5424 R — Operating manual 6 Maintenance 6.3 EN Additional service instructions for Centrifuge 5424 R Empty and clean the condensation water tray regularly and especially after liquid spillage in the rotor chamber. Pull out the tray for condensation water from the left side of the centrifuge. Operating manual Clear the rotor chamber regularly of ice formations by thawing, either by leaving the centrifuge lid open or carrying out a brief temperature control run at approx. 30 °C.
Centrifuge 5424 / 5424 R — Operating manual 7 Troubleshooting 7 Troubleshooting EN Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R Operating manual If the suggested measures fail repeatedly, please contact Technical Service. You can find the contact addresses at the end of this operating manual or on the Internet under www.eppendorf.com. 7.1 General errors Symptom / message Cause Remedy No display. No mains connection. Check the mains power connection. No display.
Centrifuge 5424 / 5424 R — Operating manual 7 Troubleshooting 7.2 EN Error messages If one of the following error messages appears, proceed as follows: Operating manual 1. Remove fault (see Remedies). 2. If necessary, repeat centrifugation. Symptom / message Cause Remedy LID ERROR Centrifuge lid cannot be locked. Try to close the centrifuge lid again. LID ERROR Centrifuge lid cannot be released. 1. Switch the device off and back on. 2. Press the open key. If the error occurs again: 1.
Centrifuge 5424 / 5424 R — Operating manual 7 Troubleshooting EN 7.3 Emergency release Operating manual If the centrifuge lid cannot be opened, you can activate the emergency release manually. Risk of injury from rotating rotor. Wait for the rotor to stop before activating the emergency lid release. To check, look through the inspection glass in the centrifuge lid. WARNING! To operate the emergency release, use the rotor key supplied with the Centrifuge 5424 / 5424 R. Hint! 1.
Centrifuge 5424 / 5424 R — Operating manual 8 Transport, storage and disposal 8 8.1 Transport, storage and disposal Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R EN Transport Air temperature 8.2 8.3 Rel. humidity Air pressure General transportation -25 to 60 °C 10 to 75% 30 to 106 kPa Air freight -20 to 55 °C 10 to 75% 30 to 106 kPa Air temperature Rel.
Centrifuge 5424 / 5424 R — Operating manual 9 Technical data 9 EN 9.1 Technical data Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R Power supply Centrifuge 5424 Operating manual Mains power connection 230 V, 50 to 60 Hz 120 V, 50 to 60 Hz 100 V, 50 to 60 Hz Current consumption: 1.9 A (230 V) 3.8 A (120 V) 4.0 A (100 V) Power consumption: max.
Centrifuge 5424 / 5424 R — Operating manual 9 Technical data 9.3 EN Weight / dimensions Centrifuge 5424 Width: 236 mm (9.30 in.) Operating manual Dimensions: Depth: 320 mm (12.60 in.) Height: 227 mm (8.94 in.) Weight without rotor: 13.4 kg (29.5 lb) Noise level: < 51 dB(A) * Centrifuge 5424 R Dimensions: Width: 290 mm (11.42 in.) Depth: 480 mm (18.90 in.) Height: 260 mm (10.24 in.) Weight without rotor: 21.0 kg (46.
Centrifuge 5424 / 5424 R — Operating manual 9 Technical data EN 9.4.2 Centrifuge 5424 R Run time: 30 s to 9:59 h, infinitely (oo), Operating manual adjustable up to 10 min. in 0.5 min. increments, thereafter in 1 min. increments. Temperature: -10°C to 40°C Relative centrifugal force (RZB or rcf): 1 to 21,130 x g, adjustable in 50 x g increments. Speed: 100 to 15,000 rpm, adjustable in 100 rpm increments. 36 Max. load: 24 micro test tubes of 2.0 mL each. Max.
Centrifuge 5424 / 5424 R — Operating manual 10 Ordering information 10 Ordering information Operating Centrifuge on see p. Tab. Fig. —p. manual 5424 / 5424 R EN 10.1 Centrifuge 5424 5424 000.215 - 5424 000.010 - 5424 000.614 - 5424 000.410 - Order No. Description (North America) 022620452 022620498 Centrifuge 5424 with keypad, without rotor 230 V / 50 - 60 Hz 120 V / 50 - 60 Hz, with US-plug 022620461 022620444 Centrifuge 5424 with keypad, incl.
Centrifuge 5424 / 5424 R — Operating manual 10 Ordering information EN 10.3 Accessories Operating manual 10.3.1 Rotors and rotor lids Order No. (International) Order No. Description (North America) 5424 702.007 022653008 Rotor FA-45-24-11 aerosol-tight*, aluminum, 45° angle, 24 places, max. tube diameter 11 mm, incl. rotor lid (aluminum) 5424 703.003 022653024 Replacement lid for rotor FA-45-24-11 aerosol-tight*, aluminum 5424 700.
Centrifuge 5424 / 5424 R — Operating manual Declarations and Certificates 8 176 Declarations and Certificates Operating Centrifuge on see p. Tab. Fig. —p.
Declarations and Certificates 177
Declarations and Certificates &HUWLILFDWH 1XPEHU 20091210-E215059 5HSRUW 5HIHUHQFH E215059-A1-UL-1 ,VVXH 'DWH 2009 December 10 Issued to: Eppendorf A G Barkhausenweg 1 Hamburg, 22339 Germany This is to certify that representative samples of Laboratory Use Electrical Equipment 5424R Have been investigated by Underwriters Laboratories in accordance with the Standard(s) indicated on this Certificate.
Declarations and Certificates 179
Declarations and Certificates 180
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