User`s guide

4 Quick Reference
The steps necessary for operating Gatan CCD camera are summarized here.
• Make sure that the viewing chamber is at the proper vacuum.
• Make sure the water and the three color-coded pressure lines are
correctly connected to the camera and proper water pressure is
maintained.
• Switch on the CCD camera controller.
• Flip the Peltier Cooler switch to the COOL position. For best results it
is recommended waiting (2 hours or more) for the system to stabilize.
• Flip the shutter switch to AUTO.
• Turn the computer on (if it is not on yet) and double click in the
DigitalMicrograph icon to launch the application.
• Check the TEM session information by clicking on the Global Info
button on the Image Info palette (Figure 3-3).
• Run the Set Bias Levels routine by selecting the Set Bias Levels button
under the Camera menu.
• Prepare a quadrant correction reference by choosing Prepare
Quadrant Correction Reference under the Camera menu (Figure 2-
3).
• Prepare a gain reference image by choosing Preparing Gain
Reference under the Camera menu (Figure 2-3).
UltraScan User’s Guide Reference Series Rev 1.0 4-1