User`s guide

Quadrant Correction Reference
Figure 2-4 Set the target intensity
2.4 Quadrant Correction Reference
The above Set Bias Levels routine removes gross contrast variation from
quadrant to quadrant. The remaining variation can be removed by the Prepare
Quadrant Correction Reference routine.
Before running the Prepare Quadrant Correction Reference routine, make
sure the CCD has reached its stable temperature (reading from the front panel
of the First Light controller).
Remove the sample from the field of view and spread the illumination evenly
across the CCD sensor (similar to “prepare gain reference” image).
Under Camera menu (Figure 2-3a), run the Prepare Quadrant Correction
Reference routine. Set the target intensity so the center of the intensity
indicator is in the green region (Figure 2-4). Follow the instructions on the
screen. This is a time consuming procedure as images with various exposures
are recorded to cover full intensity range. It typically takes about 50 and 75
UltraScan User’s Guide Reference Series Rev 1.0 2-6