Instruction manual

Accessories included:

Variable speed pump 120 V, ground isolated. Flow rate 1 liter/min, typi-

cal

Casting stand 14 cm x 13 cm

Comb 10 well comb and comb holder

Tygon tubing 365 cm

Disposable sample plug mold 50 slot

Yeast DNA Standard

S. cerevisiae

YNN295, 2 plugs

Chromosomal grade agarose 5 grams

Pulsed field certified agarose 5 grams

Leveling bubble 1

Fuses 0.5 Amp Fast Blow, 2 spares

Manual 1

Screened cap 1

Cooling Module (Optional):

Weight 14 kg

Construction Aluminum

Dimensions 42 cm long x 23 cm wide x 24 cm high

Cooling capacity 75 watts of input power at 14 °C

Operating range 5 °C–25 °C

Total System Weight 41.7 kg

Note: This equipment complies with the limits for a Class A digital device, pursuant to Part

15 of the FCC rules. These limits provide reasonable protection against harmful interference

when the equipment is operated in a commercial environment. This equipment generates,

uses, and can radiate, radio frequency energy and, if not installed and used in accordance with

the instruction manual, may cause harmful interference to radio communications. Operation

of this equipment in a residential area is likely to cause harmful interference, and the user

will be required to correct the interference at his own expense.

1.4 Description of Major Components

Power Module

The Power Module contains the electronics for pulsed field electrophoresis, including a

350 V power supply, the switching functions, and drivers for the 24 electrodes. The drivers

provide clamped homogeneous electric fields in the electrophoresis cell, and maintain them

regardless of the field angle selected. This dynamic regulation feature modulates the poten-

tials so that the proper voltages are maintained regardless of gel size, or fluctuations in buffer

conductivity or temperature. The fused power supply operates with a maximum voltage gra-

dient of 9 V/cm, or 300 V. The lowest gradient is 0.6 V/cm, or 20 V.

Figure 1.1A shows the relative potentials of each electrode pair when the + 60° vector

(indicated by the arrow) is activated. Net field vector is from NW to SE. The highest poten-

tials are along the SE segment of the hexagon. The potentials gradually decline along the

adjacent segments. The NW segment, directly opposite the SE, has 0 potential, represented in

the diagram as negative terminals. When the - 60° angle is activated, the pattern of electric

charges is as shown in Figure 1.1B. Together, the two pulses result in a 120° included field

angle. Other angles will result in values for the relative electrode potentials, according to pre-

determined values.