Instruction manual

5.5 Separations of DNA Size Standards
1. Restriction fragments
Size Range: 0.2–23 kb
Agarose: 1.2% Molecular Biology Certified
Buffer: 0.5x TBE
Temperature: 14 °C
Switch Time: 0.1 second
Run Time: 3 hours
Angle: 120°
Voltage Gradient: 9 V/cm
2. 5 kb Ladder
Size Range: 5–75 kb
Agarose: 1.0% Molecular Biology Certified
Buffer: 0.5x TBE
Temperature: 14 °C
Switch Time: 1-6 seconds
Run Time: 11 hours
Angle: 120°
Voltage Gradient: 6 V/cm
3. Lambda Ladder
Size Range: 50–1,000 kb
Agarose: 1.0% Molecular Biology Certified
Buffer: 0.5x TBE
Temperature: 14 °C
Switch Time: 50-90 seconds
Run Time: 22 hours
Angle: 120°
Voltage Gradient: 6 V/cm
4. Saccharomyces cerevisiae
Size Range: 240–2,200 kb
Agarose: 1.0% Pulsed Field Certified
Buffer: 0.5x TBE
Temperature: 14 °C
Switch Time: 60–120 seconds
Run Time: 24 hours
Angle: 120°
Voltage Gradient: 6 V/cm
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