User manual
22
Culture Myograph System Model 202CM
2.3.3 Setup and Control of Superfusion Flow
The superfusion circuit consists of an inow and an outow from the culture
myograph chamber. Both ows are driven by the same peristaltic pump, which leads
the superfusion buffer from the reservoir to the culture myograph chamber and nally
to a waste bottle. The small steel pipe on the culture myograph chamber cover is
connected to a sterile micro lter (0.20µm). The small pipe works as a breathing valve
to prevent over pressure in the culture myograph chamber.
The superfusion buffer reservoir is continuously aerated with a mixture of 95%
O
2
and 5% CO
2
. The gas passes through a sterile micro lter (0.20µm) to prevent
contamination of the sterile superfusion buffer.
Peristaltic Pump
Waste Bottle
Culture Myograph Chamber
Oxygen
Supply
Superfusion
Buffer
Micro Filters
Figure 2.4 Illustration of perfusion ow control