Specifications
Software Specications
27
(Note)
UV Validation SoftwareUVProbe Software
Operating
System
Data
Acquisition
Modes
General
Spectrum
Mode
Data
Processing
in Spectrum
Mode
Photometric
(Quantitation)
Mode
Windows 7 Professional (64/32 bits)
Spectrum, Photometric (quantitation), and Kinetics (time course)
• Multitasking (possible to execute data processing while
measurement is being performed)
• Customizable measurement screen layout (wavelengths, data
display font and font size, colors, displayed number of digits)
• GLP/GMP compliant (security, history)
• Real-time concentration display
• Comparison/interactive processing of multiple spectra
(Note 2)
• Storage of all processed data with original data set including
a history of all manipulations
• Spectrum enlargement/shrinking and auto scale
• Automatic interlink with spectral data processing
• Annotation on spectrum screen
• Normalization, Point Pick, peak/valley detection, area calculation
• 1st – 4th derivatives, smoothing, reciprocal, square root, natural log,
Abs, %T conversion, exponential conversion, and Kubelka-Munk
conversion
• Ensemble averaging, interpolation, four arithmetic operations
(between spectra, between spectra and factors)
• Single wavelength, multi-wavelength (includes 1, 2, or 3
wavelengths), spectrum quantitation (peak, maximum, minimum,
area, etc. for specied wavelength ranges)
• K-factor, single-point, multi-point calibration curves (1st, 2nd, 3rd
order function tting, pass-through-zero specication)
• Photometric processing with user-dened functions (+, − , × , ÷ ,
Log, Exp, etc. functions, including factors)
• Weight correction, dilution factor correction, and other corrections
using factors
• Averaging of repeat measurement data
• Simultaneous display of standard sample table, unknown sample
table, and calibration curves
• Display of Pass/Fail indications
Kinetics
(Time Course)
Mode
Report
Generator
• Comparison/interactive processing of multiple time-course data
(Note 2)
• Single- or double-wavelength measurement (difference or ratio)
• Simultaneous display of time-course data, enzyme table, and graphs
• Enzyme kinetics calculation (for single- or multi-cell)
• Michaelis-Menten calculations and graph creation (Michaelis-Menten,
Lineweaver-Burk, Hanes, Woolf, Eadie-Hofstee), Dixon plot, Hill plot
• Integrated management of sample information including original
data, sample weight, and dilution factors, etc.
• Event recording such as addition of reagents during measurement
• Time-course spectrum data processing (same as in spectrum data
processing)
1) The PC, CRT, and printer are not included as standard.
2) Depends on the specications (including memory capacity) of the PC used.
As a guideline, 20 to 30 sets of spectral data can be handled simultaneously.
General requirements for UVProbe to operate are indicated below.
• 1 GB min. of empty hard disk space
• XGA or better video adapter and monitor, with a recommended resolution of at
least 1024 × 768 pixels
• USB port
• Graphics printer
• Mouse or similar pointing device
• CD-ROM drive
Even with the above conguration, UVProbe operating performance cannot be
guaranteed, depending on Windows settings, hardware state, etc.
Use Shimadzu recommended equipment, if possible.
• Preview and print functions for customized formats
• Layout and editing of templates
• Quick printing using report templates
• Auto-printing (spectrum mode)
• Multi-page printout
• Insertion of date, time, text, and drawing objects including lines,
circles and rectangles
• Insertion of spectral/quantitative data, method, and history
• Insertion of headers and footers
• Specication of graph line thickness (for each module), font style,
and size
• Initialization results log
• Wavelength accuracy
• Wavelength repeatability
• Photometric accuracy
• Photometric repeatability
• Resolution
• Stray light
• Baseline atness
• Noise level
• Drift (baseline stability)
Inspection
Items
• Select the items to implement during inspection to conrm the
approximate time required for full inspection.
• The system is capable of both wavelength repeatability checks
and wavelength accuracy checks using a wavelength calibration
lter. If the optional mercury lamp unit is used, wavelength
accuracy can be inspected using the low-pressure mercury lamp's
bright line.
• The software is capable of resolution inspections using methods
dened in the EP (European Pharmacopoeia) and USP (United
States Pharmacopoeia) as well as the method for checking the
bright line spectral bandwidth.
Features










