Agilent 1260 Infinity Quaternary Pump VL User Manual Agilent Technologies
Notices © Agilent Technologies, Inc. 2010 Warranty No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws. The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions.
In This Guide... In This Guide... This manual covers the Agilent 1260 Infinity Quaternary Pump VL (G1311C). 1 Introduction This chapter gives an introduction to the pump, instrument overview and internal connectors. 2 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
In This Guide... 8 Test Functions and Calibration This chapter describes the tests for the module. 9 Maintenance This chapter describes the maintenance of the module. 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance. 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. 12 Appendix This chapter provides addition information on safety, legal and web.
Contents Contents 1 Introduction 9 Introduction to the Quaternary Pump 10 Overview of the Hydraulic Path 11 Early Maintenance Feedback 17 Instrument Layout 18 Electrical Connections 19 Interfaces 21 Setting the 8-bit Configuration Switch 27 2 Site Requirements and Specifications 35 Site Requirements 36 Physical Specifications 39 Performance Specifications 40 3 Installing the Pump 43 Unpacking the Quaternary Pump 44 Optimizing the Stack Configuration 46 Installing the Quaternary Pump 49 Connecting Mo
Contents 6 Troubleshooting and Diagnostics 75 Overview of the Module’s Indicators and Test Functions Status Indicators 78 User Interfaces 80 Agilent Lab Advisor Software 81 7 Error Information 76 83 What are Error Messages 85 General Error Messages 86 Module Error Messages 92 8 Test Functions and Calibration Introduction 108 System Pressure Test Leak Rate Test 111 9 Maintenance 107 109 113 Introduction to Maintenance 114 Warnings and Cautions 115 Overview of Maintenance 117 Cleaning the Module 118
Contents 10 Parts and Materials for Maintenance 147 Pump Head Assembly Without Seal Wash 148 Pump Head Assembly with Seal Wash Option 150 Outlet Valve 152 Purge Valve Assembly 153 Active Inlet Valve Assembly 154 Accessory Kit 155 Seal Wash Option Kit 156 Solvent Cabinet 157 Bottle Head Assembly 158 Hydraulic Path 159 11 Identifying Cables 161 Cable Overview 162 Analog Cables 164 Remote Cables 166 BCD Cables 169 CAN/LAN Cables 171 External Contact Cable 172 Agilent Module to PC 173 Agilent 1200 Module t
Contents 8 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 1 Introduction Introduction to the Quaternary Pump 10 Overview of the Hydraulic Path 11 How Does the Pump Work? 12 How Does Compressibility Compensation Work? How Does Variable Stroke Volume Work? 15 Early Maintenance Feedback Instrument Layout 15 17 18 Electrical Connections 19 Serial Number Information (ALL) Rear View of the Module 20 Interfaces 21 Overview Interfaces 20 23 Setting the 8-bit Configuration Switch 27 Communication Settings for RS-232C 30 Special Set
1 Introduction Introduction to the Quaternary Pump Introduction to the Quaternary Pump The quaternary pump comprises a solvent cabinet, a vacuum degasser and a four-channel gradient pump. The four-channel gradient pump comprises a high-speed proportioning valve and a pump assembly. It provides gradient generation by low pressure mixing. A solvent cabinet provides enough space for four one-liter bottles.
Introduction Overview of the Hydraulic Path 1 Overview of the Hydraulic Path The quaternary pump is based on a two-channel, dual piston in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by one pump assembly which can generate pressure up to 400 bar.
1 Introduction Overview of the Hydraulic Path KVXjjb X]VbWZg 9Z\VhhZg ;gdb hdakZci WdiiaZh 9VbeZg Id hVbea^c\ jc^i VcY Xdajbc >caZi kVakZ DjiaZi kVakZ Id lVhiZ Figure 2 Hydraulic Path of the Quaternary Pump How Does the Pump Work? The liquid runs from the solvent reservoir through the degasser to the MCGV and from there to the inlet valve. The pump assembly comprises two substantially identical piston/chamber units.
Introduction Overview of the Hydraulic Path 1 100 µL depending on the flow rate. The microprocessor controls all flow rates in a range of 1 µL/min–10 mL/min. The inlet of the first piston/chamber unit is connected to the passive inlet valve. The outlet of the first piston/chamber unit is connected through the outlet valve and the damping unit to the inlet of the second piston/chamber unit. The outlet of the purge valve assembly is then connected to the following chromatographic system.
1 Introduction Overview of the Hydraulic Path there it moves back for a defined distance. The controller stores this piston position in memory. After this initialization the quaternary pump starts operation with the set parameters. The passive inlet valve opens and the down-moving piston draws solvent into the first chamber. At the same time the second piston moves upwards delivering to the system.
Introduction Overview of the Hydraulic Path 1 How Does Compressibility Compensation Work? The compressibility of the solvents in use will affect retention-time stability when the back pressure in the system changes (for example, ageing of column). In order to minimize this effect, the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type. The compressibility compensation is set to a default value and can be changed through the user interface.
1 Introduction Overview of the Hydraulic Path The module uses a processor-controlled spindle system for driving its pistons. The normal stroke volume is optimized for the selected flow rate. Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume. By default, the stroke volume for the pump is set to AUTO mode. This means that the stroke is optimized for the flow rate in use. A change to larger stroke volumes is possible but not recommended.
Introduction Early Maintenance Feedback 1 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.
1 Introduction Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed. This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet.
1 Introduction Electrical Connections Electrical Connections • The CAN bus is a serial bus with high speed data transfer. The two connectors for the CAN bus are used for internal module data transfer and synchronization. • One analog output provides signals for integrators or data handling systems. • The interface board slot is used for external contacts and BCD bottle number output or LAN connections.
1 Introduction Electrical Connections Serial Number Information (ALL) The serial number information on the instrument labels provide the following information: CCXZZ00000 Format CC Country of manufacturing (DE Germany) X Alphabetic character A-Z (used by manufacturing) ZZ Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Rear View of the Module Figure 4 20 Rear view of the pump 1260 Inf
Introduction Interfaces 1 Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces: Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN/BCD (optional) LAN (on-board) RS-232 Analog APG Remote Special G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump 2 Yes No Yes 1 Yes G4220A/B Bin Pump 2 No Yes Yes No Yes G1361A Prep Pump 2 Yes No Yes No Yes CAN-DC- OUT for CAN slaves
1 Introduction Interfaces Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN/BCD (optional) LAN (on-board) RS-232 Analog APG Remote Special G4212A/B DAD 2 No Yes Yes 1 Yes G1315C DAD VL+ G1365C MWD G1315D DAD VL G1365D MWD VL 2 No Yes Yes 2 Yes G1321B FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes G1316A/C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No No AUX G4227A Flex Cube
1 Introduction Interfaces Overview Interfaces CAN The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. LAN The modules have either an interface slot for an LAN card (e.g. Agilent G1369A/B LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD). This interface allows the control of the module/system via a connected PC with the appropriate control software.
1 Introduction Interfaces Table 3 RS-232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI >chigjbZci BVaZ Figure 5 E8 ;ZbVaZ ;ZbVaZ BVaZ RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board.
Introduction Interfaces 1 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements. The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired- or technique).
1 Introduction Interfaces Table 4 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. 3 START (L) Request to start run / timetable. Receiver is any module performing run-time controlled activities. 4 SHUT DOWN (L) System has serious problem (for example, leak: stops pump).
1 Introduction Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch (On-Board LAN) The 8-bit configuration switch is located at the rear of the module. Switch settings provide configuration parameters for LAN, serial communication protocol and instrument specific initialization procedures. All modules with on-board LAN, e.g. G1315/65C/D, G1314D/E/F, G4212A/B, G4220A: • Default is ALL switches DOWN (best settings) - Bootp mode for LAN.
1 Introduction Setting the 8-bit Configuration Switch Table 5 8-bit Configuration Switch (with on-board LAN) Mode Function SW 1 SW 2 0 0 LAN SW 3 SW 4 SW 5 SW 6 Link Configuration SW 7 SW 8 Init Mode Selection Auto-negotiation 0 x x x x x 10 MBit, half-duplex 1 0 0 x x x 10 MBit, full-duplex 1 0 1 x x x 100 MBit, half-duplex 1 1 0 x x x 100 MBit, full-duplex 1 1 1 x x x Bootp x x x 0 0 0 Bootp & Store x x x 0 0 1 Using Stored x x x 0 1
1 Introduction Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch (without On-Board LAN) The 8-bit configuration switch is located at the rear of the module. Modules that do not have their own LAN interface (e.g. the TCC) can be controlled through the LAN interface of another module and a CAN connection to that module.
1 Introduction Setting the 8-bit Configuration Switch Table 6 NOTE 8-bit Configuration Switch (without on-board LAN) Mode Select 1 2 RS-232C 0 1 Reserved 1 0 TEST/BOOT 1 1 3 4 5 Baudrate 6 7 Data Bits 8 Parity Reserved RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369A/B. Refer to the documentation provided with the card.
Introduction Setting the 8-bit Configuration Switch Table 8 1 Baudrate Settings (without on-board LAN) Switches Baud Rate 3 4 5 0 0 0 0 0 0 0 Table 9 Switches Baud Rate 3 4 5 9600 1 0 0 9600 1 1200 1 0 1 14400 1 0 2400 1 1 0 19200 1 1 4800 1 1 1 38400 Data Bit Settings (without on-board LAN) Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 10 Parity Settings (without on-board LAN) Switches Parity 7 8 0 0 No Parity 1 0 Odd
1 Introduction Setting the 8-bit Configuration Switch Special Settings The special settings are required for specific actions (normally in a service case). NOTE The tables include both settings for modules – with on-board LAN and without on-board LAN. They are identified as LAN and no LAN. Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part).
Introduction Setting the 8-bit Configuration Switch Table 12 1 Forced Cold Start Settings (without on-board LAN) Mode Select SW1 SW2 SW3 SW4 SW5 SW6 SW7 SW8 LAN TEST/BOOT 1 1 0 0 0 0 0 1 No LAN TEST/BOOT 1 1 0 0 1 0 0 1 1260 Infinity QP VL User Manual 33
1 34 Introduction Setting the 8-bit Configuration Switch 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 2 Site Requirements and Specifications Site Requirements 36 Physical Specifications 39 Performance Specifications 40 This chapter provides information on environmental requirements, physical and performance specifications.
2 Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 13 on page 39. Consequently there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.
2 Site Requirements and Specifications Site Requirements CAUTION Unaccessable power plug. In case of emergency it must be possible to disconnect the instrument from the power line at any time. ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Power Cords Different power cords are offered as options with the module. The female end of all power cords is identical.
2 Site Requirements and Specifications Site Requirements WA R N I N G Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment. ➔ Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. Bench Space The module dimensions and weight (see Table 13 on page 39) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.
2 Site Requirements and Specifications Physical Specifications Physical Specifications Table 13 Physical Specifications Type Specification Weight 11 kg (25 lbs) Dimensions (height × width × depth) 180 x 345 x 435 mm (7.0 x 13.
2 Site Requirements and Specifications Performance Specifications Performance Specifications Table 14 40 Performance Specification Agilent 1260 Infinity Quaternary Pump VL (G1311C) Type Specification Hydraulic system Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons Setable flow range 0.001 – 10 mL/min, in 0.001 mL/min increments Flow range 0.2 – 10.0 mL/min Flow precision ≤0.07 % RSD, or ≤ 0.
2 Site Requirements and Specifications Performance Specifications Table 14 Performance Specification Agilent 1260 Infinity Quaternary Pump VL (G1311C) Analog output For pressure monitoring, 2 mV/bar, one output Communications Controller-area network (CAN), RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional 1260 Infinity QP VL User Manual 41
2 42 Site Requirements and Specifications Performance Specifications 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 3 Installing the Pump Unpacking the Quaternary Pump 44 Damaged Packaging 44 Delivery Checklist 44 Accessory Kit G1311-68755 45 Optimizing the Stack Configuration One Stack Configuration 46 Installing the Quaternary Pump 46 49 Connecting Modules and Control Software 52 Connecting Agilent 1260 Infinity Modules 52 Connecting Control Software and/or G4208A Instant Pilot Flow Connections of the Quaternary Pump 53 54 Priming the System 57 Initial Priming 57 Regular Priming 5
3 Installing the Pump Unpacking the Quaternary Pump Unpacking the Quaternary Pump Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. CAUTION "Defective on arrival" problems If there are signs of damage, please do not attempt to install the module.
Installing the Pump Unpacking the Quaternary Pump Table 15 3 Quaternary Pump Checklist Description Quantity Column Eclipse Plus C18, 4.6 x 100 mm, 3.5 µm (p/n 959961-902) (optional) 1 Column Poroshell 120 EC-C18, 4.5 x 50 mm, 2.7 µm (p/n 699975-902) (optional) 1 Column SB-C18, 4.6 x 150 mm, 5 µm (p/n 883975-902) (optional) 1 HPLC Starter Kit incl. 0.17 mm i.d. cap (p/n G4201-68707) (optional) else: HPLC Starter Kit incl. 0.12 mm i.d.
3 Installing the Pump Optimizing the Stack Configuration Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume.
Installing the Pump Optimizing the Stack Configuration 3 HdakZci XVW^cZi Ejbe AdXVa jhZg ^ciZg[VXZ 6jidhVbeaZg I]ZgbdhiViiZY Xdajbc XdbeVgibZci 9ZiZXidg Figure 8 1260 Infinity QP VL User Manual Recommended Stack Configuration (Front View) 47
3 Installing the Pump Optimizing the Stack Configuration 68 edlZg 86C Wjh XVWaZ id adXVa jhZg ^ciZg[VXZ GZbdiZ XVWaZ 86C Wjh XVWaZ A6C id Xdcigda hd[ilVgZ adXVi^dc YZeZcYh dc YZiZXidg 6cVad\ YZiZXidg h^\cVa & dg ' djiejih eZg YZiZXidg Figure 9 48 Recommended Stack Configuration (Rear View) 1260 Infinity QP VL User Manual
Installing the Pump Installing the Quaternary Pump 3 Installing the Quaternary Pump Parts required # p/n 1 Pump 1 1 Description Data System G4208A 1 Instant Pilot Power cord For other cables see text below and “Cable Overview” on page 162. Preparations WA R N I N G • • • Locate bench space. Provide power connections. Unpack the module. Module is partially energized when switched off, as long as the power cord is plugged in. Repair work at the module can lead to personal injuries, e.g.
3 Installing the Pump Installing the Quaternary Pump 1 Place the module on the bench in a horizontal position. 2 Ensure the power switch on the front of the module is OFF (switch stands out). HiVijh aVbe CVbZ eaViZ EdlZg hl^iX] HZg^Va cjbWZg Figure 10 Front of Pump 3 Connect the power cable to the power connector at the rear of the module.
3 Installing the Pump Installing the Quaternary Pump 4 Connect the required interface cables to the quaternary pump, see “Connecting Modules and Control Software” on page 52. 8dc[^\jgVi^dc hl^iX] Hadi [dg ^ciZg[VXZ WdVgY 6cVad\ egZhhjgZ 6E< gZbdiZ GH"'('8 86C EdlZg 5 Connect all capillaries, solvent tubes and waste tubing (see “Flow Connections of the Quaternary Pump” on page 54). 6 Press the power switch to turn on the module.
3 Installing the Pump Connecting Modules and Control Software Connecting Modules and Control Software WA R N I N G Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
3 Installing the Pump Connecting Modules and Control Software Connecting Control Software and/or G4208A Instant Pilot NOTE With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. The preferred communication is LAN. NOTE Usually the detector is producing the most data in the stack, followed by the pump, and it is therefore highly recommended to use either of these modules for the LAN connection.
3 Installing the Pump Flow Connections of the Quaternary Pump Flow Connections of the Quaternary Pump Parts required Preparations WA R N I N G # Description 1 Other modules 1 Parts from accessory kit 2 wrenches 1/4 - 5/16 inch for capillary connections Pump is installed in the LC system Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks.
3 Installing the Pump Flow Connections of the Quaternary Pump 2 Place the solvent cabinet on top of the quaternary pump. 3 Put the bottle-head assemblies into solvent reservoirs containing your mobile phase and place the bottle in the solvent cabinet. 4 Connect the inlet tubes from the bottle-head assemblies to the inlet connectors A to D (typically the left connection of the channel) of the vacuum degasser. Fix the tubes in the tube clips of the pump.
3 Installing the Pump Flow Connections of the Quaternary Pump 9 Prime your system before first use (see “Initial Priming” on page 57).
3 Installing the Pump Priming the System Priming the System Initial Priming When Before a new degasser or new solvent tubing can be used, it is necessary to prime the system. Isopropanol (IPA) is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties. Parts required # Description 1 Isopropanol Preparations Connect all modules hydraulically as described in the respective module manuals.
3 Installing the Pump Priming the System NOTE When priming the vacuum degasser with a syringe, the solvent is drawn through the degasser tubes very quickly. The solvent at the degasser outlet will therefore not be fully degassed. Pump for approximately 10 minutes at your desired flow rate before starting an analysis. This will allow the vacuum degasser to properly degas the solvent in the degasser tubes. 1 Open the purge valve of the pump 2 Set the flow rate to 5 mL/min.
3 Installing the Pump Priming the System Regular Priming When When the pumping system has been turned off for a certain time (for example, overnight) air will rediffuse into the solvent channel between the vacuum degasser and the pump. Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time.
3 Installing the Pump Priming the System Changing Solvents When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents are immiscible or one solvent contains a buffer) it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.
Installing the Pump Priming the System Table 16 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation When switching between reverse phase and normal phase (both times) Isopropanol Isopropanol Best solvent to flush air out of the system Miscible with almost all solvents After an installation Ethanol or methanol Alternative to isopropanol (second choice) if no isopropanol is available To clean the system when using buffers After changing aqueous solvents
3 62 Installing the Pump Priming the System 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 4 Using the Quaternary Pump Hints for Successful Use of the Quaternary Pump Prevent Blocking of Solvent Filters 64 65 Algae Growth in HPLC Systems 66 How to Prevent and/or Reduce the Algae Problem 67 This chapter provides information for optimized usage of the quaternary pump.
4 Using the Quaternary Pump Hints for Successful Use of the Quaternary Pump Hints for Successful Use of the Quaternary Pump • Always place the solvent cabinet with the solvent bottles on top of the quaternary pump (or at a higher level). • When using salt solutions and organic solvents in the Agilent 1260 Infinity Quaternary Pump VL it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports.
Using the Quaternary Pump Prevent Blocking of Solvent Filters 4 Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module. This is especially true for aqueous solvents or phosphate buffers (pH 4 to 7). The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module.
4 Using the Quaternary Pump Algae Growth in HPLC Systems Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems. Algae grow in aqueous media, preferably in a pH range of 4-8. Their growth is accelerated by buffers, for example phosphate or acetate. Since algae grow through photosynthesis, light will also stimulate their growth. Even in distilled water small-sized algae grow after some time.
4 Using the Quaternary Pump Algae Growth in HPLC Systems Symptoms Observed with the Agilent 1260 Infinity HPLC In contrast to the HP 1090 and HP 1050 Series HPLC systems which use helium degassing, algae have a better chance to grow in systems such as the Agilent 1260 Infinity where helium is not used for degassing (most algae need oxygen and light for growth).
4 68 Using the Quaternary Pump Algae Growth in HPLC Systems 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 5 Optimizing Performance Operational Hints for the Multi Channel Gradient Valve (MCGV) When to use the Seal Wash Option Choosing the Right Pump Seals 70 71 72 Optimize the Compressibility Compensation Setting 73 This chapter gives hints on how to optimize the performance or use additional devices.
5 Optimizing Performance Operational Hints for the Multi Channel Gradient Valve (MCGV) Operational Hints for the Multi Channel Gradient Valve (MCGV) In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations. However in the mixing point of the gradient valve, at the boundary between the two solvents, micro precipitation is possible. Gravity forces the salt particles to fall down.
5 Optimizing Performance When to use the Seal Wash Option When to use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump. The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent. The seal wash option is strongly recommended when buffer concentrations of 0.1 M or higher will be used for long time periods in the pump. The active seal wash upgrade can be ordered as G1398A.
5 Optimizing Performance Choosing the Right Pump Seals Choosing the Right Pump Seals The standard seal for the pump can be used for most applications. However applications that use normal phase solvents (for example, hexane) are not suited for the standard seal and require a different seal when used for a longer time in the pump.
5 Optimizing Performance Optimize the Compressibility Compensation Setting Optimize the Compressibility Compensation Setting The compressibility compensation default setting is 100 × 10-6 /bar for the pump. This setting represents an average value. Under normal conditions the default setting reduces the pressure pulsation to values (below 1% of system pressure) that will be sufficient for most applications and for all gradient analyses.
5 Optimizing Performance Optimize the Compressibility Compensation Setting Table 17 74 Solvent Compressibility Solvent (pure) Compressibility (10-6/bar) Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 Isopropanol 100 Methanol 120 1-Propanol 100 Toluene 87 Water 46 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions 76 Status Indicators 78 Power Supply Indicator 78 Module Status Indicator 79 User Interfaces 80 Agilent Lab Advisor Software 81 Overview about the troubleshooting and diagnostic features.
6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Overview of the Module’s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state (prerun, run, and error states) of the module. The status indicators provide a quick visual check of the operation of the module.
6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump. When a problem with the pump is suspected, use this test to help troubleshoot the pump and its pumping performance.
6 Troubleshooting and Diagnostics Status Indicators Status Indicators Two status indicators are located on the front of the module. The lower left indicates the power supply status, the upper right indicates the module status. HiVijh ^cY^XVidg EdlZg hjeean ^cY^XVidg Figure 13 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON.
6 Troubleshooting and Diagnostics Status Indicators Module Status Indicator The module status indicator indicates one of six possible module conditions: • When the status indicator is OFF (and power switch light is on), the module is in a prerun condition, and is ready to begin an analysis. • A green status indicator, indicates the module is performing an analysis (run mode). • A yellow indicator indicates a not-ready condition.
6 Troubleshooting and Diagnostics User Interfaces User Interfaces Depending on the user interface, the available tests vary. Some descriptions are only available in the service manual. Table 18 80 Test functions available vs.
Troubleshooting and Diagnostics Agilent Lab Advisor Software 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system. Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet. Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules.
6 82 Troubleshooting and Diagnostics Agilent Lab Advisor Software 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 7 Error Information What are Error Messages 85 General Error Messages 86 Timeout 86 Shut-Down 87 Remote Timeout 87 Synchronization Lost 88 Leak 88 Leak Sensor Open 89 Leak Sensor Short 89 Compensation Sensor Open Compensation Sensor Short Fan Failed 91 Open Cover 91 90 90 Module Error Messages 92 Restart Without Cover 92 Pressure Above Upper Limit 92 Pressure Below Lower Limit 93 Pressure Signal Missing 93 Valve Failed (MCGV/SSV) 94 Inlet-Valve Fuse 95 Valve Fuse 95 Miss
7 Error Information Agilent Lab Advisor Software Servo Restart Failed 100 Pump Head Missing 101 Index Limit 101 Index Adjustment 102 Index Missing 102 Stroke Length 103 Initialization Failed 103 Wait Timeout 104 Solvent Zero Counter 105 Degasser: cannot read signal 105 Degasser: limit not reached 106 This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.
7 Error Information What are Error Messages What are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook.
7 Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout The timeout threshold was exceeded. Probable cause Suggested actions 1 The analysis was completed successfully, Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required. and the timeout function switched off the module as requested.
7 Error Information General Error Messages Shut-Down An external instrument has generated a shut-down signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message. Probable cause Suggested actions 1 Leak detected in another module with a CAN Fix the leak in the external instrument before connection to the system. restarting the module.
7 Error Information General Error Messages Synchronization Lost During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated. Probable cause Suggested actions 1 CAN cable disconnected. • Ensure all the CAN cables are connected correctly.
Error Information General Error Messages 7 Leak Sensor Open The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits. If the current falls outside the lower limit, the error message is generated. Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service representative.
7 Error Information General Error Messages Compensation Sensor Open The ambient-compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes. If the resistance across the sensor increases above the upper limit, the error message is generated.
7 Error Information General Error Messages Fan Failed The cooling fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated. This limit is given by 2 revolutions/second for longer than 5 seconds. Probable cause Suggested actions 1 Fan cable disconnected. Please contact your Agilent service representative. 2 Defective fan.
7 Error Information Module Error Messages Module Error Messages These errors are pump specific. Restart Without Cover The module was restarted with the top cover and foam open. The sensor on the main board detects when the top foam is in place. If the module is restarted with the foam removed, the module switches off within 30 s, and the error message is generated. Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent service representative.
7 Error Information Module Error Messages Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit. Probable cause Suggested actions 1 Lower pressure limit set too high. Ensure the lower pressure limit is set to a value suitable for the analysis. 2 Air bubbles in the mobile phase. • Ensure solvents are degassed. Purge the module. • Ensure solvent inlet filters are not blocked. • Inspect the pump head, capillaries and fittings for signs of a leak.
7 Error Information Module Error Messages Valve Failed (MCGV/SSV) Valve 0 Failed: valve A Valve 1 Failed: valve B Valve 2 Failed: valve C Valve 3 Failed: valve D One of the valves of the multi-channel gradient valve has failed to switch correctly. The processor monitors the valve voltage before and after each switching cycle. If the voltages are outside expected limits, the error message is generated. Probable cause Suggested actions 1 Gradient valve disconnected.
7 Error Information Module Error Messages Inlet-Valve Fuse The active-inlet valve in the module has drawn excessive current causing the inlet-valve electronic fuse to open. Probable cause Suggested actions 1 Defective active inlet valve. Restart the module. If the error message appears again, exchange the active inlet valve. 2 Defective connection cable (front panel to Please contact your Agilent service representative. main board). 3 Defective main board.
7 Error Information Module Error Messages Missing Pressure Reading The pressure readings read by the pump ADC (analog-digital converter) are missing. The ADC reads the pressure signal of from the damper every 1ms. If the readings are missing for longer than 10 seconds, the error message is generated. Probable cause Suggested actions 1 Damper disconnected. Please contact your Agilent service representative. 2 Defective damper. Please contact your Agilent service representative.
Error Information Module Error Messages 7 Motor-Drive Power The current drawn by the pump motor exceeded the maximum limit. Blockages in the flow path are usually detected by the pressure sensor in the damper, which result in the pump switching off when the upper pressure limit is exceeded. If a blockage occurs before the damper, the pressure increase cannot be detected by the pressure sensor and the module will continue to pump. As pressure increases, the pump drive draws more current.
7 Error Information Module Error Messages Encoder Missing The optical encoder on the pump motor in the module is missing or defective. The processor checks the presence of the pump encoder connector every 2 seconds. If the connector is not detected by the processor, the error message is generated. Probable cause Suggested actions 1 Defective or disconnected pump encoder Please contact your Agilent service representative. connector. 2 Defective pump drive assembly.
Error Information Module Error Messages 7 Temperature Out of Range The temperature sensor readings in the motor-drive circuit are out of range. The values supplied to the ADC by the hybrid sensors must be between 0.5 V and 4.3 V. If the values are outside this range, the error message is generated. Probable cause Suggested actions 1 Defective main board. Please contact your Agilent service representative. Temperature Limit Exceeded The temperature of one of the motor-drive circuits is too high.
7 Error Information Module Error Messages Servo Restart Failed The pump motor in the module was unable to move into the correct position for restarting. When the module is switched on, the first step is to switch on the C phase of the variable reluctance motor. The rotor should move to one of the C positions. The C position is required for the servo to be able to take control of the phase sequencing with the commutator.
7 Error Information Module Error Messages Pump Head Missing The pump-head end stop in the pump was not found. When the pump restarts, the metering drive moves forward to the mechanical end stop. Normally, the end stop is reached within 20 seconds, indicated by an increase in motor current. If the end point is not found within 20 seconds, the error message is generated. Probable cause Suggested actions 1 Pump head not installed correctly (screws Install the pump head correctly. Ensure nothing (e.g.
7 Error Information Module Error Messages Index Adjustment The encoder index position in the module is out of adjustment. During initialization, the first piston is moved to the mechanical stop. After reaching the mechanical stop, the piston reverses direction until the encoder index position is reached. If the time to reach the index position is too long, the error message is generated. Probable cause Suggested actions 1 Irregular or sticking drive movement.
Error Information Module Error Messages 7 Stroke Length The distance between the lower piston position and the upper mechanical stop is out of limits (pump). During initialization, the module monitors the drive current. If the piston reaches the upper mechanical stop position before expected, the motor current increases as the module attempts to drive the piston beyond the mechanical stop. This current increase causes the error message to be generated.
7 Error Information Module Error Messages Wait Timeout When running certain tests in the diagnostics mode or other special applications, the pump must wait for the pistons to reach a specific position, or must wait for a certain pressure or flow to be reached. Each action or state must be completed within the timeout period, otherwise the error message is generated. Possible Reasons for a Wait Timeout: · Pressure not reached. · Pump channel A did not reach the delivery phase.
7 Error Information Module Error Messages Solvent Zero Counter Pump firmware version A.02.32 and higher allow to set solvent bottle fillings in the data system. If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly. Probable cause Suggested actions 1 Volume in bottle below specified volume. Refill bottles and reset solvent counters. 2 Incorrect setting. Make sure the limits are set correctly.
7 Error Information Module Error Messages Degasser: limit not reached This error is thrown, if the degasser does not become ready after 8 min, i.e. is higher than 180 mbar. Probable cause Suggested actions 1 Liquid in degasser tubing. • Wait for evaporation of liquid. • Replace tubing. • Replace degasser chamber. • Replace vacuum pump. • Replace tubing. • Replace degasser chamber. 2 Leak in degasser tubing or chamber. 3 Degasser vacuum pump defect. 106 Replace vacuum pump.
1260 Infinity QP VL User Manual 8 Test Functions and Calibration Introduction 108 System Pressure Test 109 System Pressure Test failed Leak Rate Test 111 Leak Rate Test Description 110 111 This chapter describes the tests for the module.
8 Test Functions and Calibration Introduction Introduction All tests are described based on the Agilent Lab Advisor Software B.01.03. Other user interfaces may not provide any test or just a few. For details on the use of the interface refer to the interface documentation.
8 Test Functions and Calibration System Pressure Test System Pressure Test Description The System Pressure Test is a quick, built-in test designed to demonstrate the pressure-tightness of the system. The test is required, if problems with small leaks are suspected, or after maintenance of flow-path components (e.g., pump seals, injection seal) to prove pressure tightness up to 400 bar. For running the test, please refer to the online help of the diagnostic software.
8 Test Functions and Calibration System Pressure Test System Pressure Test failed The test will fail, if the sum of all leaks in the system (pump, autosampler or column compartment and connections) exceeds the test limit. After isolating and fixing the cause of the leak, repeat the System Pressure Test to confirm the system is pressure tight. Probable cause Suggested actions 1 Purge valve open. Close the purge valve. 2 Loose or leaky fittings. Tighten the fitting or exchange the capillary.
Test Functions and Calibration Leak Rate Test 8 Leak Rate Test Leak Rate Test Description The Leak Rate Test is a built-in troubleshooting test designed to demonstrate the leak-tightness of the pump. For running the Leak Rate Test and evaluating test results, please refer to the online help of LabAdvisor.
8 112 Test Functions and Calibration Leak Rate Test 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 9 Maintenance Introduction to Maintenance Warnings and Cautions 115 Overview of Maintenance Cleaning the Module 114 117 118 Checking and Cleaning the Solvent Filter Cleaning the Solvent Filter 120 119 Exchanging the Passive Inlet Valve (PIV) 121 Exchanging the Outlet Valve 122 Exchanging the Purge Valve Frit 124 Removing the Pump Head Assembly 126 Maintenance of a Pump Head Without Seal Wash Option Seal Wear-in Procedure 128 132 Maintenance of a Pump Head
9 Maintenance Introduction to Maintenance Introduction to Maintenance The module is designed for easy repair. The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack. These repairs are described in “Overview of Maintenance” on page 117.
9 Maintenance Warnings and Cautions Warnings and Cautions WA R N I N G Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice.
9 Maintenance Warnings and Cautions CAUTION Safety standards for external equipment ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.
9 Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the pump that can be carried out without opening the main cover.
9 Maintenance Cleaning the Module Cleaning the Module The module case should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent. Do not use an excessively damp cloth as liquid may drip into the module. WA R N I N G Liquid dripping into the electronic compartment of your module. Liquid in the module electronics can cause shock hazard and damage the module. ➔ Do not use an excessively damp cloth during cleaning.
9 Maintenance Checking and Cleaning the Solvent Filter Checking and Cleaning the Solvent Filter CAUTION Small particles can permanently block the capillaries and valves of the module. Damage of the module. ➔ Always filter solvents. ➔ Never use the module without solvent inlet filter. NOTE If the filter is in good condition the solvent will freely drip out of the solvent tube (hydrostatic pressure). If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube.
9 Maintenance Checking and Cleaning the Solvent Filter Cleaning the Solvent Filter When If solvent filter is blocked Parts required Description Concentrated nitric acid (35 %) LC grade water Beaker Preparations Remove solvent inlet tube from the adapter at the inlet valve. 1 Remove the blocked solvent filter from the bottle-head assembly and place it in a beaker with concentrated nitric acid (35%) for one hour.
9 Maintenance Exchanging the Passive Inlet Valve (PIV) Exchanging the Passive Inlet Valve (PIV) When If internally leaking (backflow) Tools required • • Parts required # Description G1312-60066 Passive inlet valve Wrench 14 mm Pair of Tweezers 1 Remove the front cover. 2 Disconnect the solvent inlet tube from the inlet valve (be aware that solvent may leak out of the tube due to hydrostatic flow). 3 Using a 14 mm wrench loosen the passive inlet valve and remove the valve from the pump head.
9 Maintenance Exchanging the Outlet Valve Exchanging the Outlet Valve When If internally leaking Tools required Wrench 1/4 inch Wrench 14 mm Parts required Preparations NOTE # Description G1312-60067 Outlet valve, complete • • Switch off pump at the main power switch Remove the front cover Before exchanging the outlet valve you can try to clean it in a sonic bath for 5 – 10 min. Place the valve in an upright position in a small beaker with alcohol.
Maintenance Exchanging the Outlet Valve 4 Reinstall the outlet valve and tighten the valve. 9 5 Reconnect the valve capillary.
9 Maintenance Exchanging the Purge Valve Frit Exchanging the Purge Valve Frit When • • Frit – when piston seals are exchanged or when contaminated or blocked (pressure drop of > 10 bar across the frit at a flow rate of 5 mL/min of H20 with purge valve opened) Purge valve – if internally leaking Tools required • • • Wrench 1/4 inch Wrench 14 mm Pair of tweezers or toothpick Parts required # p/n 1 01018-22707 PTFE frit (pack of 5) 1 G1311-60009 Purge valve 1 5067-4728 Seal cap (optional)
9 Maintenance Exchanging the Purge Valve Frit 6 Place a new frit into the purge valve with the orientation of the frit as shown above. 8 Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube. 7 Reinstall the cap with the gold seal. DjiaZi XVe^aaVgn Ejg\Z kVakZ LVhiZ ijWZ 9 Tighten the purge valve and reconnect outlet capillary and waste tubing.
9 Maintenance Removing the Pump Head Assembly Removing the Pump Head Assembly When • • • Exchanging the seals Exchanging the pistons Exchanging seals of the seal wash option Tools required • • Wrench 1/4 inch 4-mm hexagonal key Preparations • • Switch off pump at the main power switch and unplug the power cable Use a solvent shutoff valve or lift up solvent filters for avoiding leakages CAUTION Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive.
9 Maintenance Removing the Pump Head Assembly Next Steps: 4 Disconnect the capillary from the outlet valve. 7 Using a 4 mm hexagonal key, stepwise loosen the two pump head screws and remove the pump head from the pump drive. 5 Remove the waste tubing and disconnect the solvent tubing from the inlet valve. 6 Remove the capillary at the bottom of the pump head.
9 Maintenance Maintenance of a Pump Head Without Seal Wash Option Maintenance of a Pump Head Without Seal Wash Option When In case of maintenance or pump head internal leaks.
Maintenance Maintenance of a Pump Head Without Seal Wash Option 1 Place the pump head on a flat surface. Loosen the lock screw (two revolutions) and while holding the lower half of the assembly carefully pull the pump head away from the piston housing. 9 2 Remove the support rings from the piston housing and lift the housing away from the pistons.
9 Maintenance Maintenance of a Pump Head Without Seal Wash Option 3 Check the piston surface and remove any deposits or layers. Cleaning can be done with alcohol or tooth paste. Replace piston if scratched. 4 Using the insert tool carefully remove the seal from the pump head. Remove wear retainers, if still present. >chZgi idda E^hidc hjg[VXZ Ejbe hZVa 5 Insert new seals into the pump head. 6 Reassemble the pump head assembly.
9 Maintenance Maintenance of a Pump Head Without Seal Wash Option Next Steps: 7 If a standard seal has been installed, run the seal wear-in procedure, see “Seal Wear-in Procedure” on page 132. 8 For the normal phase seal, the purge valve frit should be replaced, see “Exchanging the Purge Valve Frit” on page 124.
9 Maintenance Seal Wear-in Procedure Seal Wear-in Procedure Parts required CAUTION # Description 0100-1847 Adapter AIV to solvent inlet tubes 5022-2159 Restriction capillary Seal damage ➔ This procedure is required for Standard seals (pack of 2) (p/n 5063-6589), but it will damage the PE seals (pack of 2) (p/n 0905-1420). 1 Place a bottle with 100 mL of isopropanol in the solvent cabinet and place a tubing (including bottle head assembly) in the bottle.
Maintenance Maintenance of a Pump Head with Seal Wash Option 9 Maintenance of a Pump Head with Seal Wash Option When When maintaining seal wash option Tools required • Parts required Preparations 4-mm hexagonal key # p/n Description 1 01018-23702 Insert tool 1 0905-1175 Wash seal 1 5062-2484 Gasket, seal wash (pack of 6) • • • • • Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters for avoiding leakages Remove the pump
9 Maintenance Maintenance of a Pump Head with Seal Wash Option 1 Remove the seal holder and the seal wash support rings from the piston housing. Remove the seal holder from the support ring assembly. 2 Using the blade of a flat-blade screwdriver remove the seal wash gasket and the secondary seal from the support ring.
9 Maintenance Maintenance of a Pump Head with Seal Wash Option 5 Place the support rings on the piston housing (pistons not 6 Insert the pistons and carefully press them into the seal. installed) and snap the pump head and piston housing together. E^hidc Ejbe ]ZVY Hjeedgi g^c\ E^hidc ]djh^c\ 7 Tighten the lock screw.
9 Maintenance Reinstalling the Pump Head Assembly Reinstalling the Pump Head Assembly When When reassembling the pump Tools required • Parts required # Description 79846-65501 Pump head grease 4-mm hexagonal key 1 Apply a small amount of grease on the back of the 2 Slide the pump head assembly onto the pump drive. pistons.
Maintenance Reinstalling the Pump Head Assembly 3 Using a 4 mm hexagonal key tighten the pump head screws stepwise with increasing torque. 9 4 Reconnect all capillaries, tubes and (if installed) the active inlet valve cable to its connector. Ejbe ]ZVY hXgZlh 5 Reinstall the front cover.
9 Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) Exchanging the Multi-Channel Gradient Valve (MCGV) Tools required Parts required Preparations NOTE 138 Screwdriver Pozidriv #1 # p/n Description 1 G1311-69701 MCGV (exchange assembly) • • • Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters for avoiding leakages.
Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) 1 Disconnect the connecting tube, waste tube and the solvent tubes from the MCGV, unclip them from the tube clips and place them into the solvent cabinet to avoid flow by hydrostatic pressure. 9 2 Press the lower sides of the cover to unclip it. Remove the cover. LVhiZ [jccZa B8
9 Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) 5 Install the MCGV cover. Reconnect the waste funnel with 6 Reconnect the tube from the inlet valve to the middle the waste tube holder in the top cover. Insert waste tube in the holder in the leak pan and clip tube to the MCGV cover. position of the MCGV. Connect solvent tubes for channels A-D from the MCGV to the degasser outlets.
9 Maintenance Exchanging the Optional Interface Board Exchanging the Optional Interface Board When Parts required CAUTION Board defective # p/n Description 1 G1351-68701 Interface board (BCD) with external contacts and BCD outputs Electronic boards are static sensitive and should be handled with care so as not to damage them. Touching electronic boards and components can cause electrostatic discharge (ESD). ESD can damage electronic boards and components.
9 Maintenance Exchanging the Optional Interface Board 6 Reconnect the pump to line power.
Maintenance Exchanging the Active Inlet Valve (AIV) or its Cartridge 9 Exchanging the Active Inlet Valve (AIV) or its Cartridge When If internally leaking (backflow) Tools required • • Parts required # Preparations Wrench 14 mm Pair of Tweezers p/n Description 1 G1312-60025 Active inlet valve body (optional), without cartridge 1 5062-8562 Active Inlet Valve Cartridge (400 bar) 1 G1311-67304 Connecting tube, MCGV to AIV • • Switch off pump at the main power switch and unplug the power c
9 Maintenance Exchanging the Active Inlet Valve (AIV) or its Cartridge 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head. 6Xi^kZ ^caZi kVakZ WdYn KVakZ XVgig^Y\Z Figure 16 Active Inlet Valve Assembly 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly. 7 Before inserting the new valve cartridge clean the area in the actuator assembly. Flush the cartridge area thoroughly with alcohol.
Maintenance Replacing the Module Firmware 9 Replacing the Module Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary • to keep all systems on the same (validated) revision or • if a new module with newer firmware is added to a system or • if third part control software requires a special version.
9 146 Maintenance Replacing the Module Firmware 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 10 Parts and Materials for Maintenance Pump Head Assembly Without Seal Wash 148 Pump Head Assembly with Seal Wash Option Outlet Valve 152 Purge Valve Assembly 153 Active Inlet Valve Assembly Accessory Kit Solvent Cabinet 154 155 Seal Wash Option Kit 156 157 Bottle Head Assembly Hydraulic Path 150 158 159 This chapter provides information on parts for maintenance.
10 Parts and Materials for Maintenance Pump Head Assembly Without Seal Wash Pump Head Assembly Without Seal Wash Item p/n Description G1312-60064 Pump Head without Seal Wash 1 5067-4695 Sapphire piston (default) 2 G1312-60062 Piston housing (incl.
Parts and Materials for Maintenance Pump Head Assembly Without Seal Wash 10 , & ' ( ) * &% .
10 Parts and Materials for Maintenance Pump Head Assembly with Seal Wash Option Pump Head Assembly with Seal Wash Option Item p/n Description G1312-60065 Pump Head with Seal Wash 1 5067-4695 Sapphire piston (default) 2 G1312-60062 Piston housing (incl.
Parts and Materials for Maintenance Pump Head Assembly with Seal Wash Option 10 Complete pump head assembly with seal wash option includes items 1-8 and items 11, 13 and 14. &) & &% ' && * ) ( + , &( &' Figure 18 1260 Infinity QP VL User Manual .
10 Parts and Materials for Maintenance Outlet Valve Outlet Valve p/n Description G1312-60067 Outlet valve, complete Figure 19 152 Outlet Valve 1260 Infinity QP VL User Manual
Parts and Materials for Maintenance Purge Valve Assembly 10 Purge Valve Assembly Item p/n Description 1 G1312-60061 Purge valve assembly 2 01018-22707 PTFE frit (pack of 5) 3 5067-4728 Seal cap & ' ( 1260 Infinity QP VL User Manual 153
10 Parts and Materials for Maintenance Active Inlet Valve Assembly Active Inlet Valve Assembly Item p/n Description 1 G1312-60025 Active inlet valve without cartridge 2 5062-8562 Active Inlet Valve Cartridge (400 bar) & ' Figure 20 154 Active Inlet Valve Assembly 1260 Infinity QP VL User Manual
Parts and Materials for Maintenance Accessory Kit 10 Accessory Kit Accessory Kit G1311-68755 p/n Description 5062-2461 Waste tube, 5 m (reorder pack) 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m 5988-8453EN Capillary/fitting starter kit brochure 9222-0519 Bag, plastic G1329-87300 Capillary 0.
10 Parts and Materials for Maintenance Seal Wash Option Kit Seal Wash Option Kit 156 p/n Description G1311-60161 Seal wash pump assembly 5042-8507 Seal wash pump cartridge (silicone tubing) 5062-2465 Support ring, seal wash 0905-1175 Secondary seal (pre-installed in support rings) 5062-2484 Gasket, seal wash (pack of 6) 5001-3743 Seal holder 0890-1764 Silicone rubber tubing 1 mm i.d.
Parts and Materials for Maintenance Solvent Cabinet 10 Solvent Cabinet Item p/n Description 1 5065-9981 Solvent cabinet, including all plastic parts 2 5043-0207 Name plate 1260 4 5042-8567 Leak pan 5 9301-1420 Solvent bottle, transparent 6 9301-1450 Solvent bottle, amber 7 G1311-60003 Bottle-head assembly ' & ( ) , *$+ Figure 21 Solvent Cabinet Parts 1260 Infinity QP VL User Manual 157
10 Parts and Materials for Maintenance Bottle Head Assembly Bottle Head Assembly Item p/n Description G1311-60003 Bottle-head assembly 1 5063-6598 Ferrules with lock ring (10x) 2 5063-6599 Tube screw (10x) 3 Wire marker 4 5062-2483 Solvent tubing, 5 m 5 5062-8517 Inlet filter adapter (pack of 4) 6 5041-2168 Solvent inlet filter, 20 µm ( ) ' & * + Figure 22 158 Bottle-Head Assembly Parts 1260 Infinity QP VL User Manual
Parts and Materials for Maintenance Hydraulic Path 10 Hydraulic Path Item p/n Description 1 Outlet capillary, pump to injector device G1312-67305 or 1 G1329-87300 Outlet capillary, pump to thermostattable autosampler G1311-60003 Bottle-head assembly 2 G1322-67300 Kit of 4 solvent tubes for connection degasser to MCGV (Quaternary Pump) including labels 3 G1311-81600 Capillary, piston 1 to damper 4 G1311-81601 Capillary, damper to piston 2 5 5067-4693 Connecting tube, MCGV to PIV or 5
10 Parts and Materials for Maintenance Hydraulic Path ' ( ) & * + Figure 23 160 Hydraulic Flow Path of the Quaternary Pump 1260 Infinity QP VL User Manual
1260 Infinity QP VL User Manual 11 Identifying Cables Cable Overview 162 Analog Cables 164 Remote Cables 166 BCD Cables 169 CAN/LAN Cables 171 External Contact Cable Agilent Module to PC 172 173 Agilent 1200 Module to Printer 174 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.
11 Identifying Cables Cable Overview Cable Overview NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Identifying Cables Cable Overview 11 CAN cables p/n Description 5181-1516 CAN cable, Agilent module to module, 0.
11 Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made.
Identifying Cables Analog Cables 11 Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent module Signal Name Shield Shield Analog - Center Center Analog + Pin Agilent module Signal Name Agilent Module to General Purpose p/n 01046-60105 Pin 3394/6 1 1260 Infinity QP VL User Manual Not connected 2 Black Analog - 3 Red Analog + 165
11 Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to.
Identifying Cables Remote Cables 11 Agilent Module to 3396 Series III / 3395B Integrators p/n 03396-61010 Pin 33XX Pin Agilent module Signal Name 9 1 - White Digital ground NC 2 - Brown Prepare run Low 3 3 - Gray Start Low NC 4 - Blue Shut down Low NC 5 - Pink Not connected NC 6 - Yellow Power on High 14 7 - Red Ready High 4 8 - Green Stop Low NC 9 - Black Start request Low 13, 15 Active (TTL) Not connected Agilent Module to Agilent 35900 A/D Converters p/n 5061-33
11 Identifying Cables Remote Cables Agilent Module to General Purpose p/n 01046-60201 168 Pin Universal Pin Agilent module Signal Name Active (TTL) 1 - White Digital ground 2 - Brown Prepare run Low 3 - Gray Start Low 4 - Blue Shut down Low 5 - Pink Not connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop Low 9 - Black Start request Low 1260 Infinity QP VL User Manual
Identifying Cables BCD Cables 11 BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules.
11 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 170 Pin 3396 Pin Agilent module Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD0 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 +5V Low 1260 Infinity QP VL User Manual
Identifying Cables CAN/LAN Cables 11 CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables p/n Description 5181-1516 CAN cable, Agilent module to module, 0.
11 Identifying Cables External Contact Cable External Contact Cable 5 10 15 1 6 11 One end of this cable provides a 15-pin plug to be connected to Agilent modules interface board. The other end is for general purpose.
Identifying Cables Agilent Module to PC 11 Agilent Module to PC p/n Description G1530-60600 RS-232 cable, 2 m RS232-61600 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
11 Identifying Cables Agilent 1200 Module to Printer Agilent 1200 Module to Printer 174 p/n Description 5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module.
1260 Infinity QP VL User Manual 12 Appendix General Safety Information 176 The Waste Electrical and Electronic Equipment Directive Batteries Information Radio Interference Sound Emission 179 180 181 182 Solvent Information 183 Agilent Technologies on Internet 184 This chapter provides addition information on safety, legal and web.
12 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements.
Appendix General Safety Information 12 Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Some adjustments described in the manual, are made with power supplied to the instrument, and protective covers removed. Energy available at many points may, if contacted, result in personal injury.
12 Appendix General Safety Information Safety Symbols Table 21 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages. Indicates a protected ground terminal. Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product.
Appendix The Waste Electrical and Electronic Equipment Directive 12 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005. NOTE This product complies with the WEEE Directive (2002/96/EC) marking requirements.
12 Appendix Batteries Information Batteries Information WA R N I N G Lithium batteries may not be disposed-off into the domestic waste. Transportation of discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Danger of explosion if battery is incorrectly replaced. ➔ Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries.
Appendix Radio Interference 12 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
12 Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB.
Appendix Solvent Information 12 Solvent Information Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae. • Small particles can permanently block capillaries and valves. Therefore always filter solvents through 0.4 µm filters.
12 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download.
Index Index B solvent zero counter configuration one stack 105 46 8 8-bit configuration switch on-board LAN 27 without On-Board LAN 29 ball-screw drive 12 battery safety information 180 BCD cable 169 bench space 38 blockage 97 bottle head assembly 158 buffer application 64 buffer solution 10, 138 A C active inlet valve cartridge 143 active inlet valve 143, 154 active seal wash 10, 71 adapter 143 Agilent Diagnostic software 81 Agilent Lab Advisor software 81 Agilent Lab Advisor 81 Agilent on inter
Index pump error 92 error messages compensation sensor open 90 compensation sensor short 90 encoder missing 98 ignition without cover 91, 91 index adjustment 102 index limit 101 index missing 102 initialization failed 103 inlet valve fuse 95 inlet-valve missing 98 leak sensor open 89 leak sensor short 89 leak 88 missing pressure reading 96 motor drive power 97 pressure above upper limit 92 pressure below lower limit 93 pump configuration 96 pump head missing 101 remote timeout 87 selection valve failed 93
Index multi channel gradient valve (MCGV) 138 N non-operating altitude 39 non-operating temperature 39 O operating Altitude 39 operating temperature 39 operational pressure range 40 optimization stack configuration 46 outlet valve 122, 152 overview, pump 11 P packaging damaged 44 parts identification cables 161 parts active inlet valve 154 bottle head assembly 158 damaged 44 hydraulic path 159 missing 44 outlet valve 152 pump head with seal wash 150 pump head 148 solvent cabinet 157 passive inlet valve
Index forced cold start 32 specification physical 39 status indicator 79 stroke length 103 stroke volume 12, 15 synchronization lost 88 system setup and installation optimizing stack configuration T temperature limit exceeded 99 temperature out of range 99 temperature sensor 88 test functions 76 test function introduction 108 timeout 86 tools screwdriver pozidriv #1 138 troubleshooting error messages 76, 85 status indicators 76, 78 W 46 wait timeout 104 warnings and cautions 115 waste electrical and el
Index 1260 Infinity QP VL User Manual 189
www.agilent.com In This Book This manual contains service information about the Agilent 1260 Infinity Quaternary Pump VL (G1311C). The manual describes the following: • introduction, • site requirements and specifications, • installing the pump, • using the quaternary pump, • optimizing performance, • troubleshooting and test functions, • maintenance, • parts and materials for maintenance, • identifying cables, • appendix.
