User manual

90 | Droplet Digital
™
PCR Applications Guide
Droplet Digital
™
PCR Tips, Assay Considerations, and Troubleshooting
Reference
Kwok S and Higuchi R (1989). Avoiding false positives with PCR. Nature 339, 237–238.
Fig. 9.10. Mutant DNA spiked into increasing amounts of wild-type DNA. As the total added DNA
concentration increases, the positive fluorescence amplitudes decrease and the negative fluorescence
amplitudes increase.
Channel 1 amplitude
0 50,000 100,000 150,000 200,000
Event number
B07 B08 C03 C04 D03 D05 E03 E04 F05 F08 G06 G07
– + – + – +
Wild-type DNA
Mutant DNA
25,000
20,000
15,000
10,000
5,000