User manual

Droplet Digital
™
PCR Applications Guide | 77
Additional Applications
In this experiment, a single FAM assay was used (RPP30) at standard 1x final concentration
and two different HEX assays at different concentrations (Chr13q3 and Chr10q1).
Assay Chr10q1 was used at 1/2x standard concentration and assay Chr13q3 was used
at 1x final concentration in the reaction. All three assays were present along with template
in the supermix before making droplets. Using tools in QuantaSoft software to manually
group populations, the four upper droplet clusters (gray, blue, green, orange) represent
those droplets pertaining to droplets that are positive for the RPP30 assay. The blue clusters
are those droplets that are positive for the Chr10q1 assay. The green clusters are those
droplets that are positive for the Ch13q3 assay. The orange clusters are those droplets that
are positive for both Chr10q1 and Chr13q3 simultaneously. Negative droplets for the RPP30
assay are the lower four droplet clusters (gray, blue, green, orange).