User manual

4 | Droplet Digital
™
PCR Applications Guide
Droplet Digital
™
PCR
Fig. 1.5. The C1000 Touch thermal cycler provides robust performance for ddPCR experiments.
Fig. 1.6. QX200 droplet reader autosampler.
PCR Amplification
Droplets are transferred to a 96-well plate for PCR in a thermal cycler. We recommend the
C1000 Touch
™
thermal cycler with 96–deep well reaction module for PCR (Figure 1.5).
This high-performance thermal cycler has excellent temperature uniformity and settling
across all 96 wells to help ensure successful PCR.
Droplet Reading
Following PCR amplification of the nucleic acid target in the droplets, the plate containing
the droplets is placed in a QX100 or QX200 droplet reader, which analyzes each droplet
individually using a two-color detection system (set to detect FAM and HEX [or VIC]).
The autosampler of the droplet reader picks up the droplets from each well of the
PCR plate (Figure 1.6).