User manual

Droplet Digital
™
PCR Applications Guide | 1
1 Droplet Digital
™
PCR
Introduction
Droplet Digital polymerase chain reaction (ddPCR
™
) was developed to provide
high-precision, absolute quantification of nucleic acid target sequences with wide-ranging
applications for both research and clinical diagnostic applications. ddPCR measures
absolute quantities by counting nucleic acid molecules encapsulated in discrete,
volumetrically defined water-in-oil droplet partitions. Droplet Digital PCR using Bio-Rad’s
QX100
™
or QX200
™
Droplet Digital PCR system overcomes the previous lack of scalable
and practical technologies for digital PCR implementation.