User manual

Droplet Digital
™
PCR Applications Guide | 21
Fig. 2.7. Setting thresholds in a 1-D plot.
Note: Use 1-D plots for temperature gradient experiments and wells containing single
assays. For all experiments involving duplex assays, set thresholds in the 2-D plot view
(Figure 2.8).
For correct quantification of a single-color experiment, use the thresholding tools to ensure
correct designation of the populations as negatives (gray) and positives (blue or green).
The thresholding tools are on the left side of the screen. There is an autoanalysis tool as well
as two manual thresholding tools — one for individual wells and one for all selected wells.
Once you have chosen the desired tool, place the crosshair cursor over the place where you
would like to set the threshold and click on the chart. The droplets will change color when
you are done.
To analyze the data for a multiplex experiment, select Analyze and click the
2-D Amplitude tab.
Designing Droplet Digital
™
PCR Experiments