User manual

10 | Droplet Digital
™
PCR Applications Guide
Droplet Digital
™
PCR
Fig 1.14. QuantaSoft software concentration estimates. Twofold (A) and 10% (B) dilution series of S. aureus
template (n) in a constant background of human gDNA (n) run as a duplex FAM/VIC assay. The error bars
represent Poisson 95% confidence intervals.
Sample
Concentration, copies/µlConcentration, copies/µl
A
B
100,000
10,000
1,000
100
10
1
0.1
0.01
2,000
1,600
1,20 0
800
400
0
16 copies/µl
0.564 copies/µl
125 copies/µl
0.751 copies/µl
1,000 copies/µl
1.000 copies/µl
8 copies/µl
0.513 copies/µl
63 copies/µl
0.683 copies/µl
500 copies/µl
0.909 copies/µl
4,000 copies/µl
1.210 copies/µl
8,000 copies/µl
1.331 copies/µl
No template control
No template control
31 copies/µl
0.621 copies/µl
250 copies/µl
0.826 copies/µl
2,000 copies/µl
1.100 copies/µl
1,020
1,030
1,020
1,030
1,040
1,030
1,060
1,030
1,020
998
1,030
1,020
1,070
1,050 1,060 1,060
952
1,040
999
986
990
1,000
1,080
7.58
530
0
567
655
717
761
826
895
998
1,10 0
1,230
1, 360
17. 3
31.5
61
118
247
481
2,040
3,950
6,360
983
1,020