User manual

Index
Droplet Digital
™
PCR Applications Guide | 105
RT-ddPCR 3, 58–60, 82, 92, 99
RT-qPCR 57, 58, 60, 99
one-step 57
two-step 57, 60
S
salt 12, 14, 16, 42
concentration 12, 16, 42
sample 2, 3, 5–8, 10–13, 15–20,
22–24, 26, 29–32, 36, 38–41, 44,
46, 47, 49, 51–58, 60–64, 67,
71–74, 78–96
heterogeneous 39, 40
high background 55
homogeneous 39
SantaLucia 14
sequence 1, 2, 12–17, 19, 40,
43, 45–48, 51–55, 57, 65, 69, 72,
78–81, 83, 99
wild-type 47, 48
signal 12, 13, 30, 58, 60, 75
signal-to-noise 2, 12
single cell analysis 7
single nucleotide polymorphism
7, 13, 37, 46, 48, 78, 99
single-stranded DNA 88, 99
site 15, 16, 30, 40, 46, 60, 64
primer binding 15
SNP 13, 15, 37, 46, 48–50, 78, 99
solution 47, 74, 82, 85
somatic mosaicism 39
species 16, 30, 39, 46, 64, 65, 67,
68, 80
rare 67
target 30, 39
spectrophotometry 64
spectroscopy 17
A
260
17
standard curve 2, 7, 8, 28, 54
star activity 42
structure 11, 12, 15, 42, 57, 59,
60, 83, 87
secondary 11, 12, 15, 57,
59, 60, 83, 87
subsampling error 95–97
supermix 3, 17, 18, 24, 26, 28, 31,
58, 69, 75, 77, 81–83, 92, 93
supernatant 83
T
TaqMan 3, 11–13, 17, 46, 58, 62,
66, 74, 75, 83
probe 46
target 1–9, 11–21, 27–35, 37–40,
42–44, 46–48, 50, 51, 53–59, 63,
65, 69, 72, 73, 78–83, 86, 87,
95, 96
nucleic acid 1, 4, 20
technical error 36, 94, 96, 97
temperature 4, 11, 15, 19, 21,
23–25, 27, 40, 42, 43, 46, 48, 49,
59, 78–80, 83–87, 99
annealing 15, 23, 24, 27,
46, 79, 80
annealing/extension 24,
25, 27, 40, 43, 48, 78, 83, 85
denaturation 23, 85, 86
melting 11, 27, 46, 49,
78, 99
template 2, 7, 9, 10, 24, 28, 32,
41, 42, 51–53, 57–59, 64, 66, 69,
71, 73–75, 77, 81, 82
fragment length 42
synthetic 74, 75
thermal cycler 2, 4, 19, 23, 81,
84–86, 93
thymidines 82
T
m
11, 13, 14, 23, 27, 46, 48,
78, 99
enhancer 13
transgene 39
trisomy 21 39
fetal 39
tyrosine kinase 60