User manual

104 | Droplet Digital
™
PCR Applications Guide
Index
partitioning 2, 3, 7, 9, 28, 30, 33,
38, 41, 47, 53, 58, 64, 94, 96, 97
error 96, 97
PBMC 55, 56, 99
PCR 2–4, 6–8, 11, 13, 15–20,
23, 24, 27, 30, 31, 40, 47, 52, 54,
56–58, 64–69, 74, 78, 80, 81, 82,
84, 86, 88, 90, 93, 99
amplification 2, 4, 7, 20, 58,
65, 66, 69, 74
efficiency bias 2
inhibitor 15, 56, 86
Peltier device 85
peripheral blood mononuclear
cell 55, 99
pipetting accuracy 56
plate sealer 18, 84, 93
PMID 40
15131266 40
Poisson 6–10, 29, 33, 35, 36, 56,
75, 82, 84, 94, 96
error 36, 94
statistics 29, 33, 56, 96
primer 11–15, 17, 23, 24, 26, 27,
30, 31, 45, 46, 48, 57, 59, 74, 80,
83, 86, 88, 89
Primer3 13, 15, 27
Primer-BLAST 15, 80
Primer3Plus 13–15, 27
primer-dimer 11, 12, 24
probe 3, 11–14, 17, 23, 30, 31, 40,
45, 46, 48, 51, 57–60, 65, 66, 69,
75, 78–83, 86, 91–93
hydrolysis 11–13, 82
oligonucleotide 12
TaqMan 46
proto-oncogene 60
pseudogenes 40
purification 15, 42
Q
qPCR 11, 36, 37, 57, 99
QuantaSoft software 10, 19, 20,
22, 29–31, 35, 36, 39, 41, 59, 67,
69, 77, 79, 87
quantitative PCR 11, 36, 37, 51,
57, 99
quencher 12, 13
QX100 1–5, 12, 13, 16–20, 28, 53,
55, 66, 68, 69, 71, 75, 83, 91, 93
QX200 1–4, 11–13, 17–20, 24, 26,
28, 53, 66, 69, 75, 83, 91–93
R
rare detection 46, 51
assay 46
experimental design 51
rare event detection 19, 99
rare mutation detection 13, 45–47,
78, 79, 99
rare sequence detection 45, 46,
53, 55, 81, 99
rare target sequence detection
19, 99
reaction well 20, 52, 54
RED 19, 99
reference 7, 9, 19, 27–29, 37,
39–44, 46, 53, 59–61, 63, 86, 90
Ambion human brain 60
relative fluorescence units 79, 99
resolution 9, 24, 37, 38, 74
restriction digestion 16, 17, 41, 42,
46, 53, 72, 73, 87
RestrictionMapper 42
reverse transcription 57–60, 63,
74, 99
RFU 79, 99
RMD 45, 48, 51–53, 78, 99
RNA 3, 11, 15, 17, 24, 57–63, 68,
78, 87
RNase 58, 74, 75
RNaseP anchor assay 74
RPP30 17, 40, 73, 76, 77
RSD 45, 53, 54, 56, 99