User manual

Droplet Digital
™
PCR Applications Guide | 103
human epidermal growth factor
receptor 2 gene 60, 98, 99
human immunodeficiency virus
46, 99
hybridization 37, 60, 63, 98
comparative genomic 37
hydrolysis probes 3, 12, 13, 58
I
IHC 60, 62, 63, 99
intercalating dyes 3
in vitro transcribed RNA 60, 99
iScript
™
58, 60
advanced cDNA synthesis kit
for RT-qPCR 58, 60
Select cDNA synthesis kit 58
K
kinase 60
tyrosine 60
KRAS G12V 50
L
lake water 46, 54
limit of detection 47, 51, 99
limit of quantification 47, 54, 99
LNA 46, 99
locked nucleic acid 46, 99
locus 16, 37, 39, 44, 83
reference 37, 44
target 37, 39, 83
LoD 51–56, 99
LoQ 54, 55, 99
M
measurement error 30, 36, 56,
94, 95
melting temperature 11, 27, 46, 49,
78, 99
method 9, 12, 31, 36, 37, 45, 47,
57, 60, 62–64, 68, 74
nearest-neighbor 12
methylation 16, 42, 43
Mfold 15
microarrays 37
SNP-based 37
microfluidic 83
microfluidics 2, 3
mir-210 74, 75
miRNA 74, 75, 99
synthetic template 74
MIT 13, 15
mosaicism 39
somatic 39
MRGPRx1 38, 72, 73
multiplexing 24, 27, 75, 76
mutant 46–51, 88, 90
sequence 47
site 46
mutation 7, 13, 45–47, 56, 78,
79, 99
N
NaCl 46
NCBI 15, 40
NCBI gene 10556 40
NEBuffer 42, 43
next-generation sequencing 7, 16,
64, 67, 99
NGS 7, 16, 64, 67–69, 99
no template control 10, 24, 52, 71,
81, 99
NTC 52, 71, 81, 82, 99
nucleic acid 1, 2, 4, 7, 8, 15, 17,
20, 27, 45, 46, 64, 83, 86, 99
quantification 2
nucleotide 7, 12–14, 37, 46–48,
78, 83, 86, 99
O
oligo 12, 14, 82
oligo(dT) 57, 58
oligonucleotide 12
P
parameters 14, 23, 40
thermodynamic 14
particulate 83
Index