User manual

102 | Droplet Digital
™
PCR Applications Guide
Index
drug response 37
adverse 37
dsDNA 12, 24, 98
dUTP 69, 81, 92, 93, 98
dyes 3, 11–13, 24, 59
assay 59
intercalating 3
E
EEF2 61–63
electrophoresis 64, 70
endonuclease 16, 43
enhancers 13, 46
T
m
13
enzyme 16, 28, 42, 43, 46, 59, 60,
72, 80, 82, 83
4-cutter 42, 43
6-cutter 42, 43
methylation-insensitive 42
restriction 16, 28, 42, 43,
72, 80, 83
ERBB2 60–63, 98, 99
overexpression 60
EvaGreen 3, 11, 12, 24, 26, 27, 88,
89, 92, 93
exonuclease activity 12
experimental plate layout 18
F
FAM 3, 4, 6, 9, 10, 13, 19, 20, 22,
40, 46, 48, 49, 51, 59, 61, 66, 67,
73–78, 82
FFPE 17, 40, 53, 58, 60, 62, 98
FISH 60, 62, 63, 98
fluorescence 5–7, 12, 13, 20,
23–27, 29, 30, 48, 60, 63, 66–68,
75, 76, 79, 82, 88, 90, 98, 99
amplitude 7, 23–27, 29, 75,
76, 82, 88, 90
fluorescence in situ hybridization
60, 98
fluorescent 2, 12, 58
fluorophore 3, 6, 13, 29, 40, 48, 86
reporter 3
footprint 16
amplicon 16
formalin-fixed, paraffin-embedded
17, 40, 53, 58, 98
formula 7, 14, 16, 35
salt correction 14
fragmentation 16, 17, 40
G
GAPDH 62, 63
GAPDH-PL 62
GC 11–14, 86, 98
gDNA 9, 10, 16, 43, 46, 87, 98
gene expression 7, 24, 26, 46, 57,
59–63
genome 16, 17, 28, 37, 39–41, 44,
46, 48, 51, 53, 76
diploid 17, 44
haploid 16, 41, 48, 51
human 16, 37, 40, 76
genomic DNA 9, 26, 41–44, 53,
68, 98
GMO plant stock 39
guidelines 11–13, 17, 19, 52, 58,
81
design 11
H
haplotype 72
HER2 39, 60, 62, 63, 98, 99
HER2+ 62
Herceptin 60
HEX 3, 4, 6, 13, 19, 20, 22, 40, 46,
59, 66, 67, 75–77
histogram 29, 78, 79
HIV 46, 99
homologs 40, 79
hot-start 59, 81, 86
HPLC 74
human 9, 10, 16, 37, 40, 42, 44,
46, 48, 51, 52, 53, 55, 60, 68,
72–74, 76, 87, 98, 99
cells 55
human brain reference 60