User manual

Droplet Digital
™
PCR Applications Guide | 101
channel 5, 6, 9, 19, 23–27, 48–51,
59, 66, 67, 69, 74–76, 78, 79–83,
85, 87, 89, 90
color 67, 75
chloroform 10
Chr10q1 76, 77
Chr13q3 76, 77
chromosome 73, 74, 76
chromosome 6 74
chromosome 10 73, 74, 76
clonally derived Down syndrome
cell line 39
CN 37–42, 44, 72, 98
CN states 37–39
CNV 7, 19, 37–41, 43, 44, 62, 63,
72, 73, 98
composition 11
base 11
concentration 2, 6–10, 12, 14,
16–20, 22–24, 28–44, 46, 48, 49,
51, 54, 55, 58–65, 67, 68, 71,
74–77, 82–90, 95, 96
DNA 2, 17, 34, 48, 88, 90
oligonucleotide 12
salt 12, 16, 42
sample 6, 30, 61
copy number 7, 9, 16, 17, 27,
37–40, 44, 53, 62, 63, 72, 73,
87, 98
copy number variation 7, 19, 24,
37, 41, 63, 72, 98
CPD 31, 33, 34, 44, 71, 96–98
cross-reactivity 48, 51, 53, 78, 79
curve 2, 7, 8, 28, 54, 97
CV 54–56, 97, 98
CYP2D6 39
D
ddPCR 1–5, 7–9, 11, 14, 15,
17–20, 24, 26–33, 36, 38–40,
42–47, 51–54, 58–60, 62–72, 74,
75, 78, 81–83, 86, 88, 89, 91–95,
97, 98
denaturation 23, 85, 86
design 11–13, 15, 19, 40, 46, 51,
53, 59, 66, 73, 78, 80, 86
assay 11, 13, 15, 40, 46,
53, 73, 78, 86
experimental 19, 51, 53, 59
primer 11, 12, 80
detection of rare sequences 7
detection system 4, 56
two color 4
DG8 17, 18, 31, 52, 81, 83, 84, 91
digital PCR 1, 7, 30, 81
disease 7, 37
autoimmune 37
DNA 2, 3, 5–9, 11, 12, 14–17, 20,
24, 26, 28–34, 38, 41–44, 46–49,
51–58, 62–65, 68–70, 72–74, 78,
79, 81–90, 94, 96, 98, 99
digested 16, 17, 42, 84
foreign 54
genomic 9, 26, 41–44, 53,
68, 96
human 16, 46, 48, 51,
53, 74
human genomic 9, 42,
44, 68
inhibitory secondary 42
quantification 8, 46
restriction digested 28
total 54
undigested 72, 73, 83, 84
dNTP 14
double-negative droplets 6
double-positive droplets 6, 50,
51, 74
double-stranded DNA 24, 88, 98
droplet 1–9, 11, 12, 17–22, 24,
26–36, 38, 41, 43–45, 47–53, 55,
58, 64–72, 74–85, 87, 89, 91, 92,
94–98
volume 31, 32, 35
Droplet Digital PCR 1, 5–7, 11, 12,
28, 53, 58, 66, 75, 78, 83, 91, 94
Index