User manual

100 | Droplet Digital
™
PCR Applications Guide
Index
A
ABS 19, 20, 28, 29, 59, 98
absolute quantification 1, 2, 7, 8,
17, 19, 20, 28, 29, 54, 59, 68, 74
adverse drug response 37
algorithm 6, 29, 87
Poisson 6–10, 29, 33, 35, 36
Ambion 60, 62
human brain reference 60
amplicon 13, 15–17, 24, 25, 27,
28, 40, 42, 46, 51–53
reference 28, 40, 42
amplification 2, 4, 7, 11, 12, 20, 23,
30, 39
reaction 11, 12
amplitude 5–7, 9, 21, 23–27, 29,
48–51, 66, 67, 69, 74–76, 78–82,
87-90
fluorescence 7, 23–27, 29, 75,
76, 82, 88, 90
annealing temperature 15, 23, 24,
27, 46
assay 7, 9–17, 19, 21, 23, 27,
29–31, 36, 38–43, 45–54, 56, 57,
59–63, 65–67, 71, 73, 80, 82–87,
93, 94
dyes 59
hydrolysis 12
rare mutation detection 13, 79
RNaseP anchor 74
B
base composition 11
Basic Local Alignment Search
Tool 15
biomarker 47
biopsy 39, 46, 48, 53, 94
breast cancer 39
BLAST 15, 98
Primer-BLAST 80
blood draw 56
blood sample 39, 94
maternal 39
buffer 16, 18, 23, 42, 43, 58, 59,
70, 74, 93
control 18, 93
digestion 42, 43
restriction enzyme 16
C
cancer 37, 39, 48, 53, 56, 58, 60
breast 39, 60
cDNA 17, 26, 57–60, 63, 74,
75, 98
Celera 16
cell 7, 37, 39, 40, 52, 54, 55, 58,
60, 64, 99
surface receptor 60