pH Orion 8003, 8165, 8172, 8175 Orion ROSS ® Sure-Flow Electrodes INSTRUCTION MANUAL Analyze • Detect • Measure • Control™ ™
AQUAfast, Cahn, EZ Flash, Ionalyzer, ionplus, KNIpHE, No Cal, ORION, perpHect, PerpHecT, PerpHecTion, pHISA, pHix, pHuture, Pure Water, Sage, Sensing the Future, SensorLink, ROSS Ultra, Sure-Flow, TEA Analyzer, Titrator PLUS, TURBO2 and Wine Master are registered trademarks of Thermo Electron Corporation.
TABLE OF CONTENTS General Information 1 Introduction Required Equipment Required Solutions 1 2 2 Using the Electrode 3 Electrode Preparation Sample Requirements Placement of Removable Bulb Guard Sure-Flow® Junction Measuring Hints pH Calibration & Measurement General Calibration Procedure Two-Buffer Calibration Single Buffer Calibration pH Measurement Electrode Maintenance Cleaning Electrode Removal of Membrane/Junction Deposits Electrode Storage Troubleshooting 3 4 5 5 6 7 7 7 8 8 8 8 9 9 11 Tr
GENERAL INFORMATION Introduction This manual contains instructions for the ROSS™ Sure-Flow® Series of pH electrodes.
Required Equipment Meter — Any Orion pH or ion-selective meter, or other pH/lSE meter with appropriate connectors. Electrode — Combination pH Electrode, or pH and Reference Electrode Half Cells. Use the Orion 8003 ROSS™ Sure-Flow® Reference Half Cell Electrode with a ROSS Half Cell. Thermometer — Readable to ± 0.5 °C. Beakers — Plastic or glass. Magnetic Stirrer — Suggested for precision measurements. Required Solutions pH Buffers - Two are recommended for precise measurement.
USING THE ELECTRODE Electrode Preparation 1. Remove the protective shipping cap from sensing element and save for storage. 2. Clean any salt deposits from exterior by rinsing with distilled water. 3. Uncover filling hole by removing plastic sleeve and add ROSS™ Internal Filling Solution, Orion 810007, to electrode. See Figure 2. To maintain an adequate flow rate, the level of filling solution must cover the end of coil and be at least one inch above the sample level when immersed.
Sample Requirements One of the benefits of the ROSS™ pH Electrodes is that the filling solution composition may be changed depending on sample requirements because of the double junction construction. Do not use solutions containing silver. The ROSS pH Electrode Filling Solution, Orion 810007, is 3 M KCl. For solutions which precipitate in the presence of chloride ion, the ROSS pH Electrode could be filled with 10% KNO3, Orion 900003.
Placement of Removable Sleeve Bulb Guard The bulb guard can be removed for cleaning or general measurement. Grasp bulb guard and gently pull. The bulb guard will slide off the electrode exposing the pH sensing bulb and junction area. See Figure 4A. Press on the cap to open junction for easy cleaning. To replace bulb guard, gently push into place. The bulb guard is secure without additional turning or fittings. See Figure 4B. For general measurements the bulb guard can be left in place on the electrode.
Measuring Hints See Figure 5. • Always use fresh buffers for calibration. Choose buffers that are no more than 3 pH units apart. • Check electrode slope daily by performing two-buffer calibration. Slope should be 92 - 102%. • Except as noted in Sample Requirements, only use ROSS™ Internal Filling Solution, Orion 810007, for ROSS Combination pH and Reference Electrodes. Do not use any filling solution which may contain silver.
pH CALIBRATION & MEASUREMENT General Calibration Procedure For detailed calibration and temperature compensation procedures, consult your meter instruction manual. Two-Buffer Calibration This procedure is recommended for precise measurement. 1. Ensure that all buffers are at the same temperature. If samples are at varying temperatures, temperature compensation is recommended. (See meter instruction manual.) 2. Select two buffers which bracket the expected sample pH.
Single Buffer Calibration 1. Ensure that all buffers are at room temperature. If samples are at varying temperatures, temperature compensation is recommended. (See meter instruction manual.) 2. Set up meter according to meter instruction manual. 3. Rinse electrode first in distilled water and then with the buffer being used for calibration (the buffer should be near the expected sample pH). Place the electrode in the buffer. 4. Wait for a stable display.
Removal of Membrane/Junction Deposits When using the following procedures, do not immerse electrode more than 1/4" above junction. Protein — Soak in 10% pepsin in 0.1 M HCl for 15 minutes.* lnorganic — Soak in 0.1 M tetrasodium EDTA solution for 15 minutes.* Grease and Oil — Rinse with mild detergent or methanol solution.
Table 1 pH Values of Buffers at Various Temperatures Nominal value at 25 °Temperature Buffer 0 °C 5 °C 10 °C 20 °C 30 °C 1.68 1.67 1.67 1.67 1.67 1.68 3.78 3.86 3.84 3.82 3.79 3.77 4.01 4.00 4.00 4.00 4.00 4.02 6.86 6.98 6.95 6.92 6.87 6.85 7.00 7.11 7.08 7.06 7.01 6.98 7.41 7.53 7.50 7.47 7.43 7.40 9.18 9.46 9.40 9.33 9.23 9.14 10.01 10.32 10.25 10.18 10.06 9.97 40 °C 50 °C 60 °C 70 °C 80 °C 90 °C 1.69 1.71 1.72 1.74 1.77 1.79 3.75 3.75 4.
TROUBLESHOOTING Troubleshooting Guide Following is a systematic procedure to isolate the problem. The pH measuring systems can be divided into four components for ease in troubleshooting: pH Meter, Electrodes, Sample/Application, and Technique. pH Meter The meter is the component which is easiest to eliminate as a possible cause of error. Orion pH meters are provided with an instrument checkout procedure and shorting cap for convenience in troubleshooting.
Technique If trouble persists, review operating procedures. Reread calibration and measurement sections, to be sure proper technique has been followed. Assistance After troubleshooting all components of your measurement system, contact The Technical EdgeSM for Orion products. Within the United States call 1.800.225.1480, outside the United States call 978.232.6000 or fax 978.232.6031. In Europe, the Middle East and Africa, contact your local authorized dealer.
ELECTRODE CHARACTERISTICS Temperature Effects The most common cause of error in pH measurement is temperature. Ordinary electrodes drift with temperature changes. The Ross pH Electrode eliminates the stability problems associated with the use of conventional electrodes in samples of varying temperatures. There are, however, two effects of temperature change that should be kept in mind. 1. Electrode slope will change with varying temperature.
Interferences Sodium ion is the principal interference for the pH electrode, causing increasing error at higher pH (lower hydrogen ion activities) and at higher temperatures. Because the ROSS™ pH membrane is composed of special low-sodium error glass, error due to sodium is negligible when measuring at pH values less than 12. When measuring at pH values greater than 12, add the correction value from the nomograph in Figure 6 to the observed pH reading.
WARRANTY For the most current warranty information, visit www.thermo.com. The Thermo Electron Corporation, Orion products warranty covers failures due to manufacturer’s workmanship or material defects from the date of purchase by the user. User should return the warranty card and retain proof of purchase. Warranty is void if product has been abused, misused, or repairs attempted by unauthorized persons. Warranties herein are for product sold/installed by Thermo or its authorized dealers.
210Aplus™, 230Aplus™, 250Aplus™, 290Aplus™, 410Aplus™, 420Aplus™, 520Aplus™, 525Aplus™, 710Aplus™, 720Aplus™ and 920Aplus™), pHuture MMS™ Meters (Orion 535A and 555A), pH/Conductivity Meter (Orion 550A), Dissolved Oxygen Meters (Orion 805Aplus™, 810Aplus™, 850Aplus™ and 862A).
Six months from date of purchase by the user (or twelve months from date of shipment from Thermo) • Orion Flash Titration™ Probe (Orion 092518), pHuture™ Electrode (Orion 615700), pHuture MMS™ Pentrode™ (Orion 617500), Quatrode™ (Orion 617800) and Triode™ (Orion 615800), Low Maintenance Triode™ (Orion 9107BN), ORP Low Maintenance Triode™ (Orion 9179BN), and PerpHecT® Low Maintenance Triode™ (Orion 9207BN), Waterproof Triode™ (Orion 9107WP, 9107WL, 9109WL and 9109WP), QuiKcheK® Meters and Micro Electrodes.
WHETHER THE CLAIMS ARE BASED IN CONTRACT, IN TORT (INCLUDING NEGLIGENCE), OR OTHERWISE WITH RESPECT TO OR ARISING OUT OF THE PRODUCT FURNISHED HEREUNDER. REPRESENTATION AND WARRANTIES MADE BY ANY PERSON, INCLUDING ITS AUTHORIZED DEALERS, REPRESENTATIVES AND EMPLOYEES OF THERMO WHICH ALTER OR ARE IN ADDITION TO THE TERMS OF THIS WARRANTY SHALL NOT BE BINDING UPON THERMO UNLESS IN WRITING AND SIGNED BY ONE OF ITS OFFICERS.
ORDERING INFORMATION Electrodes Orion Description 800300 ROSS™ Sure-Flow® Reference Half Cell Electrode, Glass Body, with pin-tip connector 8165BN ROSS Sure-Flow Combination pH Electrode, Epoxy Body with BNC Connector 8172BN ROSS Sure-Flow combination pH Electrode, Glass Body with BNC Connector 8175BN ROSS Sure-Flow Semi-micro combination pH Electrode, Epoxy Body with BNC connector Accessories Orion Description 090032 Adaptor: BNC electrode to U.S.
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SPECIFICATIONS pH Range 0 - 14 Drift Less than 0.005 pH per day Temperature Range 0 to 100 °C Internal Reference ROSS™ Junction Sure-Flow® liquid-to-liquid Slope 92 to 102% of theoretical Nernst slope Isopotential Point pH 7.0 ± .1 Accuracy of Measuring a pH 6.86 Buffer After Standardization at 25 °C Accurate within 0.03 pH for buffer at any other temperature between 0-100 °C, using automatic temperature compensation Speed of Response of pH 6.86 Buffer between 25 °C and 75 °C Response stable to 0.
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