User`s manual

66 Chapter 6: Sample Experiments
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 67
User’s Manual
program main screen (Figure
6-7). Enter the ligand and
macromolecule concentrations
(based on the spectroscopic
determination) and the cell
volume. Check the Constant
Cell Volume box. To subtract
the blank run, click on the
Area label at the top of the
third table column to highlight
the entire column, then type
in an equal sign followed by
a minus sign (=-) followed by
the average blank injection
area, then click the execute
button to the right of the edit
box. This will subtract the
average blank injection area
from all peak areas. After you
have made all necessary en-
tries, click the Recalc button.
Next, select the Modeling
tab from the program main
screen (Figure 6-8). Select the
Independent model at the top
of the tab. You are likely to
notice that the integrated heat
for the rst injection is smaller
in magnitude that would be
expected by comparison to
subsequent injections. This
is caused by diffusion across
Figure 6-7: Reviewing the integrated heats for the RNase A
experiment.
Figure 6-6: Adjusting the baseline for the RNase A
experiment.