User`s manual

60 Chapter 6: Sample Experiments
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 61
User’s Manual
Figure 6-1: Filling the N-ITC III cell.
Figure 6-2: Loading the syringe into the
burette drive.
Number of injections 20
Rinse the calorimeter cell three times with Tris solution and then load the cell (Figure
6-1). The reference cell may be lled with degassed water. Allow the cells to thermally
equilibrate until the heat reading on the calorimeter is stable.
Load the 100 μL syringe with the 1.00 mM HCl solution, making sure to remove any
bubbles from the syringe. Wipe the needle with a tissue and then screw the syringe com-
pletely into the burette drive as shown in Figure 6-2. (Note: Before inserting the syringe
into the burette drive, verify that the plunger indicator on the graduated handle is in the
fully raised position. Otherwise, mount the burette on the N-ITC III without a syringe
College Publishing, Philadelphia), p. 228 ff).
Experiment Setup
Experiment parameters:
Equilibration time 200 s
Time between injections 200 s
Injection size 5 μL