User`s manual

iv Table of Contents
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 1
User’s Manual
Integration of the peak .........................................................49
Modeling and Fit of the Data...............................................50
Modeling .................................................................................51
Modeling Overview .............................................................51
Designing an Experiment ......................................................55
CHAPTER SIX: SAMPLE EXPERIMENTS ................... 59
Experiment Overview............................................................59
Experiment Walk-Through...................................................60
Introduction............................................................................60
Chemical Calibration.............................................................60
Heat of Protonation of Tris Base..........................................60
Sample Experiment................................................................63
Binding of 2’-CMP to RNase A...........................................63
CHAPTER SEVEN: TROUBLESHOOTING.................. 69
Minimizing Blank Corrections .............................................69
Operation Away from Ambient Temperature......................69
Stirring Speeds .......................................................................69