User`s manual

52 Chapter 5: Software Description
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 53
User’s Manual
V - is the volume of the cell
[L] - is the total Ligand concentration
[M] - is the protein concentration
(For more information see Freire E.;
Mayorga O.; Straume M. Analytical
Chemistry, vol. 62, NO 18, 1990.)
Two Sets of Multiple Binding Sites
This model assumes the macromolecule
has many independent binding sites (at
least two). Each site can be one of the two
types. Each type has its characteristics
(binding constant and enthalpy). The
reaction pathways are shown in Figure
5-26.
Figure 5-26: Reaction pathways for multiple
independent binding sites.
2K1
1/2K1
2K2
2K1
2K2
2K2
1/2K1
1/2K2
2K1
1/2K2
1/2K1
1/2K2