User`s manual

50 Chapter 5: Software Description
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 51
User’s Manual
Modeling
Modeling Overview
Each model contains a set of variable parameters. Curve tting produces optimum values
for these parameters. These values will best represent the given input data set.
In addition to user-dened binding models, BindWorks supports three intrinsic binding
models:
1. Independent Set of Multiple Binding Sites.
2. Two Sets of Multiple Binding Sites.
3. Cooperative Model.
Intrinsic models are “hard-wired” in BindWorks and cannot be changed or replaced.
Independent Set of Multiple Binding Sites
This is the most common model for a binding experiment. The analytical solution for the
total heat is determined by the formula:
Where the variable parameters for the model are:
∆H - is the enthalpy of binding
K - is the binding constant
n - is the number of binding sites
Other variables include:
Equation 5-3
+++
+=
K
LKKLKnMKnM
LHVQ
2
][4)][][1(][1
][
2