User`s manual

46 Chapter 5: Software Description
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 47
User’s Manual
2. Select File⇒Open from
the main menu.
3. Locate the data le from
the appropriate le folder
and click OK.
4. The data le is loaded into
BindWorks. A titration
data le of RNase A
– 2’ CMP is shown in the
example.
When your data le is loaded,
BindWorks will create a
baseline based on the data
before and after the injection.
To view the calculated
baseline single-click the View
Baseline icon from the toolbar. The calculated baseline will appear, superimposed in blue
on your data set (Figure 5-20).
If the baseline is unsuitable, single-click the Zoom icon on the toolbar. BindWorks will
parse your data, label each injection, and add nodes to the calculated baseline (Figure
5-21).
A simple, intuitive series of data “markers” denote the current location of a sliding zoom
window. Use these markers to zoom in on the area(s) you wish to change. Each parsed
injection is numbered with an injection label. The width and position of the injection
window can be moved around the injection point as required. Also visible are the nodes
used to calculate the position of the baseline.
To change the position of the node, position the mouse pointer over the node, press and
hold the left mouse button, and move the node to the desired location. You can also add
or delete nodes using the add or delete nodes icons on the toolbar.
Figure 5-20: A sample data le and the position of the View
Baseline icon.