User`s manual

42 Chapter 5: Software Description
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 43
User’s Manual
slowly to allow air bubbles to evacuate
through the top of the cell. When liquid
is just visible at the opening of the access
tube, continue to apply positive pressure
to the syringe while slowy withdrawing it
from the cell to maintain the ll level and
prevent new bubbles from being intro-
duced into the cell (Figure 5-15a). When
using aqueous solutions the reference
cell (side access tube) should be lled
with water. Make sure that the reference
needle is inserted into the reference cell
access tube after lling (Figure 5-15b).
2. Load the 100 µL syringe with the ligand
solution making sure to remove any
bubbles from the barrel of the syringe,
but leaving a small 5 to 10 µL air gap be-
tween the plunger tip and the liquid in the
barrel. Wipe the needle with a tissue and
Figure 5-15b: Sample (center) and reference
(right side) cell access tubes. Liquid should
be just visible at the bottom of the conical
overow reservoir when the cell is lled.
Figure 5-14: The lling syringe used to ush
and ll the cells.
Fill Level
Conical Overflow Reservoir
Holds Excess Liquid as
Titrant is Added
Figure 5-15a: Side view of sample cell and
access tube showing ll level and overow
reservoir.