User`s manual
42 Chapter 5: Software Description
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 43
User’s Manual
on the data tab my be enlarged by holding down the left mouse button and dragging a
window around the desired region. To restore the full data display click once on the
“Zoom Out” icon which appears above the display as
.
Show baseline
Appears on the display as . This icon displays a basic baseline that follows the
contours of the data.
Subtract baseline
Appears on the screen as
. Selecting this icon visually corrects the displayed data for
baseline drift. There is no actual change in the data le itself. Deselecting the icon will
restore the actual data display.
Show Area
Appears on the toolbar as
. This icon displays the integrated areas of each peak in µJ.
System Tab and Diagnostics Tab
The System and Diagnostics feature tabs are used for evaluation purposes by CSC
technicians and only appear when the “Debug” option is selected in Settings.
Performing a Titration using ITCRun
Refer to Chapter 4: Hardware Description for additional information about preparing the
sample and reference cells, mounting the burette drive and syringe, and establishing a
stable baseline with the stirrer rotating.
1. Using the lling syringe, ush the sample cell several times using the same buffer
solution in which the sample is prepared. After ushing, remove all of the buffer and
load the sample into the sample cell (middle access tube) inside the N-ITC III (Figure
5-14). Complete loading will take approximately 1.3 mL of solution. Fill the cell