User`s manual

38 Chapter 5: Software Description
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 39
User’s Manual
Buret • Move Down
The Buret Move Down command moves the buret to the fully lowered position. Also
appears on the toolbar as .
Help Menu
Figure 5-8 shows the help menu. If a help le is not available, it
will be implemented in later revisions of ITCRun.
Help • About ITCRun...
The Help About ITCRun command displays the software version number and other
information about ITCRun.
Feature Tabs
Feature tabs control all operations of the Isothermal Titration Calorimeter. These tabs
are used to set up key parameters of your experiment and aid in the automation of the
experiment process.
Setup Tab
Figure 5-9 shows the Setup tab. This tab establishes general experiment parameters.
Change or modify the values to suit your requirements. Experiments should be set up
based on the expected results. Typically, experiments will be twenty injections of 5 µL
when using the 100 µL syringe, and twenty-ve injections of 10 µL when using the 250
µL syringe. To collect data in either titration mode or calibration mode, single-click the
Go icon from the toolbar, or select Experiment⇒Start from the Main Menu. The user will
be prompted for an experiment lename, and the program indicator on the Setup tab will
turn red indicating that the program has begun.
Stirring Rate (rpm) & Stirrer On
This number should typically remain at 150 rpm, although rates of 200-250 rpm may also
Figure 5-8: The Help
Menu.