User`s manual

20 Chapter 3: Theory of Operation
Calorimetry Sciences Corp.
CSC 5300 N-ITC III 21
User’s Manual
Freire, E., Mayorga, O. L. & Straume, M. (1990). Isothermal Titration
Calorimetry. Anal. Chem. 62, 950A-959A.
Wiseman, T., Williston, S., Brandts, J. F. & Lin, L.-N. (1989). Rapid
Measurement of Binding Constants and Heats of Binding Using a New
Titration Calorimeter. Anal. Biochem. 179, 131-137.
Wyman, J. & Gill, S. J. (1990). Binding and Linkage: The Functional
Chemistry of Biological Macromolecules (University Science Books, Mill
Valley).
Fitting of Calorimetric Data
Values for K and ∆H are obtained by tting the integrated heats from a titration
experiment to Equation 3-1 or Equation 3-2. Use of Equation 3-2 is simpler and faster
as it requires only one calculation of the excess enthalpy for each injection whereas
Equation 3-1 requires two. In general, use of Equation 3-2 presents no problem, however
by using Equation 3-2, the error from all the previous injections is propagated into
each value of the cumulative heat. Better statistics are therefore obtained by tting the
individual heats according to Equation 3-1.