User manual

56-3124-15 Edition AF 2003-08
BioPilot, FPLC
Fraction marks
Some fraction marks are occasionally omitted when the fraction collector has been
started with the instruction Frac:Start.
Action: For version 2.0 of a standard strategy, use the instruction Collect instead.
Pressure limit
When the pressure limit set in UNICORN is exceeded, it may take about one second
before a run is paused.
Action: For a faster response to pressure changes set the pressure limit on the pumps also.
Superloop (only for Multipurpose strategy)
If the flow rate is changed during execution of the instruction Superloop, the time for
filling or emptying Superloop™ is not adjusted to the new flow rate.
Action: Do not change the flow rate when filling or emptying the Superloop.
Creating methods
When creating new methods for BioPilot and FPLC systems, you have to set Technique
to Any to be able to select appropriate templates.
Action: No action possible.
Creating new templates
When creating new templates for BioPilot and FPLC systems, you have to set Column to
Any in order to save as a new template
Action: No action possible
Changing column
If you change column in a method made for a BioPilot or FPLC system, there will not be
a question about Column Value Update as with ÄKTAdesign systems.
Action: No action possible.
ActionIfPCFail setting
The system setting called ActionIfPCFail defines the action to be taken in case of broken
communication between PC and system. Either the method is aborted or continues.
Action: No action possible. In case of broken communication between PC and system,
the system will “freeze” and continue to work with same flow etc. until the
communication is working again.