User manual

10 56-3124-15 Edition AF 2003-08
FractionCollector instruction
When a previous FractionCollector command with FracSize > 0 ml has been executed
and the Outlet valve has been switched manually the FractionCollector instruction will
not always set the Outlet valve to position F2 to redirect the flow path to the fraction
collector.
Action: Insert a FractionationStop instruction or a FractionCollector instruction with
FracSize = 0 ml immediately before the FractionCollector instruction set to FracSize > 0
ml. Alternatively, insert an OutletValve = F2 instruction immediately after this
instruction.
Fractionation after SystemWash
If you, in a method or manually, after a SystemWash instruction set a flow rate of 10 ml/
min (for ÄKTA 10 ml systems) or 25 ml/min (for ÄKTA 100 ml systems), followed by a
Fractionation instruction, the fractionation will not work properly.
Action: Use a flow rate just under or above 10 ml/min and 25 ml/min for the respective
ÄKTA system (for example, 9,9 ml/min and 24,9 ml/min).
ÄKTAsystem fraction collector 900 and 950
Self-oscillation of the outlet valve
If a FractionationStop instruction is given, and a new fraction instruction is then initiated
within the following six seconds, self-oscillation of the outlet valve may occur (The outlet
valve alters between Waste and F2).
Action: Set the FractionationStop and the following instruction further apart.
This self-oscillation may also appear during a peak fractionation.
Action: Enable both elution and peak fractionation.
Frac-950 fraction collector
The final tube in the Frac-950 cannot be defined in Run Setup within the Method Editor.
Action: Define the final tube in the method Start Protocol within System control.
Tubes for the Frac-950 18 mm rack
Some 18 mm tubes may not fit for the Frac-950 18 mm rack.
Action: Following tubes are recommended for the Frac-950 18 mm rack:
Falcon 15 ml 17x120mm
Sarstedt 10 ml 16x100mm
Sarstedt 15 ml 17x120mm
Borex 16x100mm
Elkay 15ml
TPP 15 ml
Cerbo 17x100mm
LP-Italiano 16x100mm