Specifications

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2. Click on the [Calibration] tab.
a. Select [Multi Point] in the Type box and [Fixed Wavelength] in the Formula box.
b. Select [WL224.0] in the WL1 box. In the Order of Curve box, select [1
st
]
3. Click on the [Instrument Parameters] tab and ensure that the Measuring Mode is set to
[Absorbance], and click [Close].
B. Read the Standard and view a Calibration Curve
1. Click anywhere in the Standard table to activate it. Enter the appropriate
standards and their concentration values into the table.
2. Use the Photometer Button bar to read the samples.
3. Place the first standard into the sample compartment. Click the [Read Std.]
Button.
NOTE: When the following message is displayed, click [Yes].
4. The spectrophotometer will slew to the wavelength, measure the absorbance, and
UV Probe will enter the (WL224) values into the Standard table.
5. Place each sample into the compartment and take a reading.
C. To Read Unknown Samples
1. Click anywhere on the Sample table to activate it for unknown readings. Enter
names of the unknowns into the Sample ID column. Place the first unknown into
the sample compartment of the spectrophotometer.
2. Click the [Read Unk.] button.
3. Repeat this process for the remaining samples. The concentration values are
based on the Standard Calibration Curve.
12. Kinetics
The UVProbe Kinetics module has many basic features that will assist the beginning customer
and many advanced features that will appeal to the expert users. This procedure details how to
set up a basic kinetics run.
A. To Create a Data Collection Method
1. Select [Method] from the [Edit] pull down menu. Click on the [Measurement] tab
and input the following conditions:
a. Enter [120] for the Total Time.
b. In the Type box under Wavelengths (nm), verify that [Single wavelength] is
selected as the wavelength type.
c. Enter [550] in the WL1 box to measure the sample at 550nm