Service manual

PN 4237242C
2.1-5
INSTRUMENT DESCRIPTION
SYSTEM OVERVIEW
2
Aspirate Table
The aspirate cycle, Table 2.1-2, is the most important table for the instrument. It is used for
sample analysis during a sample, control, calibration, and reproducibility run.
Table 2.1-2 Aspiration Cycle
Time
(seconds) Activity Requested
0.0 Move aspirate probe up and turn on vacuum pump.
1.0 Read Hgb Blank #2, move aspirate probe to aspirate position, drain WBC bath and move aspirate syringes
down.
2.5 Drain RBC bath and move aspirate probe down.
5.0 Display aspirate message and wait for
Aspirate
key to be pressed.
5.0 Aspirate 12
µ
L and rinse WBC bath.
6.0 Wipe aspirate probe while moving it up, drain WBC bath and rinse RBC bath.
8.5 Prefill WBC bath with 1.5 mL of diluent while moving aspirate probe to WBC bath.
10.0 Move aspirate probe down into WBC bath.
11.0 Take Hgb Blank #1 reading, dispense sample and diluent while draining vacuum isolator chamber (VIC).
12.0 Move aspirate probe up and mix initial WBC dilution.
14.0 Move aspirate probe down.
15.0 Aspirate 100
µ
L of initial WBC dilution, fill diluent reservoir, charge diluent syringe and drain RBC bath.
18.5 Move aspirate probe up.
19.5 Deliver lyse while moving aspirate probe to RBC bath and create lyse line air gap.
20.0 Move aspirate probe down while prefilling RBC bath with 0.5 mL.
21.0 Dispense RBC sample and diluent with mix bubbles.
25.0 Move aspirate probe up and apply vacuum to WBC aperture.
26.0 Apply vacuum to RBC aperture, open sweep flow and begin data accumulation.
29.0 Data accumulation takes 12 seconds with up to 24 seconds of extended count.
41.0 Turn off sweep flow and increment cycle counter.
41.5 Take Hgb readings, drain WBC bath, fill diluent reservoir and analyze data.
44.0 Rinse WBC bath, drain RBC bath, fill diluent reservoir and turn off vacuum.
46.0 Drain WBC bath, rinse RBC bath, fill diluent reservoir and begin print process.
53.0 Rinse WBC bath, drain VIC and fill diluent reservoir.
57.0 Drain VIC and overfill diluent reservoir.
60.0 Aspiration cycle is complete.
*
The system extends the RBC aperture count period if there is insufficient Plt data. It uses 3-second count periods until
there is sufficient Plt accumulation or a maximum of eight extra count periods have been performed.
C