Service manual

PN 4237242C
4.31-1
SERVICE AND REPAIR PROCEDURES
LYSE VOLUME ADJUSTMENT/VERIFICATION
4
4.31 LYSE VOLUME ADJUSTMENT/VERIFICATION
Note: This procedure need not be performed frequently. The solenoid pump is very accurate
and stable. Once adjusted correctly, it should not require readjustment. Any drift or variability
in the volume is most likely caused by a problem elsewhere in the lyse system.
Tools/Supplies Needed
B Lyse volume-adjustment measuring vial, PN 5415483-6, or a 5-mL graduated measuring
device
B 9/16 in. or adjustable wrench
B Large (1/4 in.) flat-blade screwdriver
Procedure
1. At the Main Menu, select 5 SPECIAL FUNCTIONS tt 3 DILUTER FUNCTIONS tt 3 DRAIN.
2. Disconnect the lyse pump output tubing from the Y-fitting below the WBC bath.
3. Insert the open end of the output tubing into a waste container.
4. At the Diluter Functions menu, select
2 DISPENSE LYSE. Dispense lyse once if the system
is primed, or as many times as necessary to prime the system if it is unprimed.
5. When there are no bubbles in the lyse system, insert the output tubing into an empty
measuring vial and dispense lyse 10 times.
6. Check the meniscus of the lyse:
r If the meniscus does not fall within the two indicating lines on the vial (the lines
represent 4100 and 4200 µL), adjust the lyse pump. Go to step 7.
r If the meniscus is within the two indicating lines on the vial, go to step 11.
7. To adjust the pump:
a. Loosen the adjustment screw locknut at the bottom of the lyse pump.
b. Turn the adjustment screw clockwise to decrease volume or counterclockwise to
increase volume.
Note: The adjustment is very coarse, approximately 80 µL for each turn of the screw.
8. When satisfied with the adjustment, tighten the locknut, trying not to turn the
adjustment screw.
9. Rinse and dry the vial.
10. Dispense lyse once into the waste container, then dispense 10 times into the empty
measuring vial to verify correct volume. Repeat the adjustment if the volume is incorrect.
11. Reconnect the lyse tubing, dispense lyse twice, then select
4 RINSE.
12. Perform an SVP (Heading 5.1).
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