Service manual

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PN 4237242C

4.28-1

SERVICE AND REPAIR PROCEDURES

AIM ADJUSTMENT

4.28 AIM ADJUSTMENT

Tools/Supplies Needed

B Five different whole-blood specimens with normal parameters (especially for WBC)

Note: Since nomal parameters do not guarantee a normal AIM result, it is preferable to

use different blood.

Procedure

1. Bleach the apertures. Refer to the How to Clean the Baths procedure in the Operator’s

Guide.

2. Run each of the five specimens once. After each sample, access the Service Report (see

Heading 7.2, GENERATING A SERVICE REPORT), and print or record the measured

26th-percentile AIM reading for RBC and WBC.

3. Average the five, measured, 26th-percentile readings for RBC and WBC. These results are

your new “target values.”

4. Verify that the RBC Target (T) value falls within the 2150 to 2550 range and that the

WBC Target (T) value falls withing the 2100 to 2500 range. If either are outside of these

ranges, there is an instrument problem that must be resolved before continuing.

If the new target values are within

±25 of the current (old) target values, do not adjust.

If adjustment is necessary, access the Service Report screen (see Heading 7.2,

GENERATING A SERVICE REPORT), and enter the new target values.

Optional Procedures

Option 1

Controls and calibrators have different physical properties from fresh whole blood. The 26th

percentile readings that they produce can also differ, and generate AIM alerts if the differences

are great enough. If the WBC 26th percentile is too low, as is the case with COULTER 4C

PLUS Abnormal Low cell control, target adjustments up to 2% are allowed. There is no

allowance for the WBC 96th percentile reading being too high, or for the RBC 26th percentile

reading being too high or too low.

1. Obtain a WBC 26th percentile average for fresh whole blood, following the procedure

above.

2. Run the control (Abnormal Low if 4C cell control is being used) five times and average

the WBC 26th percentile readings.

3. Average the fresh blood average and the control average. This is the same as averaging all

10 runs. Compare this result to the fresh blood average less 2% (fresh blood average x

0.980). Adjust to the higher value.

4. If 4C Abnormal Low cell control was used, it is acceptable to adjust to 1.02 x the 4C

Abnormal Low cell control average.