Service manual

PN 4237242C
4.27-1
SERVICE AND REPAIR PROCEDURES
LATEX GAIN ADJUSTMENT
4
4.27 LATEX GAIN ADJUSTMENT
Tools/Supplies Needed
B Latex aperture gain particles, PN 6857371-8
Procedure
1. Ensure that the instrument is primed and ready to run a sample.
2. At the Main Menu, select
5 SPECIAL FUNCTIONS
tt
5 SERVICE
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1 LATEX GAIN.
3. Enter the latex particle size from the assay value stated on the container.
4. Make sure the latex aperture gain particles are well mixed, then aspirate them as directed
on the display.
5. Compare the WBC and RBC results to the latex assay value. A display of Counts, CVs
and Sizes is given for WBC and RBC.
r Verify that the RBC CV is ≤8 and the WBC CV is ≤16. Results not meeting this
criteria should not be used for adjustments.
r Adjustment is necessary if either parameter is not within 1.5 fL of the latex assay
value. Press
Enter to advance to the Adjustment menu.
6. The Adjustment menu displays the new aperture current setting.
r Do not adjust the WBC if is it is outside the range of 99.6 to 140.4 V. Do not adjust
the RBC if is it is outside the range of 124.5 to 175.5 V.
r If you cannot adjust within range, there is a problem that requires correction.
r To leave the Adjustment menu without making an adjustment, press
Escape.
7. Run controls to verify calibration. Changes in aperture gain affect MCV and MPV the
most. Histogram shifts also occur.
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