C COULTER® MD II™ Series Analyzer Service Manual 7 4 1 _ 8 5 2 0 9 6 3 .
LEGAL NOTICES Coulter Corporation makes no representation that, upon furnishing this service manual, the holder of the manual will have the necessary technical capabilities and know-how to properly troubleshoot and repair any of the equipment specified in the manual.
C REVISION STATUS Initial Issue A, 8/95 Software Version 1.2 Revision B, 3/96 Software Version 1.3 Updated text for software level 1.3, updated the parts list, added specifications and procedures for the optional Epson® TM-290P Slip Printer, added information about the ISL option, and made minor corrections. The following pages were changed or added: vi, vii-xv, 2-ii, 2-iii, 2.13, 2.2-2, 2.4-5, 2.4-9, 2.4-12, 2.4-13, 3-i, 3.1-2, 3.2-1, 3.2-3, 3.4-1 through 3.4-4, 4.28-1, 4.28-2, 5.1-1, 5.2-1, 7.4-1, 8-i, 8.
This document applies to the latest software listed and higher versions. When a subsequent software version affects the information in this document, the changes will be summarized on a Notice of Information Update form and will be released by service memo.
C CONTENTS LEGAL NOTICES REVISION STATUS, i CONTENTS, iii 1 2 PN 4237242C INTRODUCTION, 1.1-1 1.1 MANUAL DESCRIPTION, 1.1-1 Scope, 1.1-1 Organization, 1.1-1 Numbering Format, 1.1-1 Special Headings, 1.1-1 WARNING, 1.1-1 CAUTION, 1.1-2 IMPORTANT, 1.1-2 ATTENTION, 1.1-2 Note, 1.1-2 Conventions, 1.1-2 1.2 SAFETY PRECAUTIONS, 1.2-1 Electronic, 1.2-1 Biological, 1.2-1 Troubleshooting, 1.2-2 INSTRUMENT DESCRIPTION, 2.1-1 2.1 SYSTEM OVERVIEW, 2.1-1 Description, 2.1-1 Software Menu System, 2.
CONTENTS Test Points, 2.2-8 Jumpers, 2.2-8 iv 2.3 FLUIDICS PANEL, 2.3-1 Overview, 2.3-1 Diluter Panel, 2.3-2 Probe/Wipe Traverse Assembly, 2.3-4 Peristaltic Pump Assembly, 2.3-5 Syringe Assembly, 2.3-6 Individual Components Mounted to Fluidics Panel, 2.3-7 Cycle Counter and Air Solenoids, 2.3-7 Diluent Reservoir, 2.3-7 Flex Connect Card, 2.3-7 Lyse Pump (PM5), 2.3-8 Solenoid Interconnect Card, 2.3-8 Vacuum Regulator, 2.3-9 2.4 SYSTEM CONTROL, 2.4-1 AT Motherboard, 2.4-1 Switches and Jumpers, 2.
CONTENTS 3 2.5 DATA ACQUISITION, 2.5-1 Sensor Preamp Adapter (SPA) Card, 2.5-1 Inputs, 2.5-1 Outputs, 2.5-2 Adjustments, 2.5-2 Jumpers, 2.5-2 Hgb Preamp Card, 2.5-3 Inputs, 2.5-4 Outputs, 2.5-4 Adjustments, 2.5-4 Test Points, 2.5-4 Vacuum Sensor Card, 2.5-4 Inputs, 2.5-5 Outputs, 2.5-5 Adjustments, 2.5-5 Test Points, 2.5-6 Sensor Processing Adapter with Diagnostics (SPAD) Card, 2.5-6 Power Supply Conditioning, 2.5-7 Oscillator Circuit, 2.5-7 Control Functions, 2.
CONTENTS 3.3 INSTRUMENT VERIFICATION, 3.3-1 Startup the Instrument, 3.3-1 Calibrate the Instrument, 3.3-1 PART B: UPGRADE AND OPTION INSTALLATION 3.4 4 vi OPTIONAL TICKET PRINTERS, 3.4-1 Epson TM-290P Slip Printer, 3.4-1 Purpose, 3.4-1 Single-Printer Setup Procedure, 3.4-1 Two-Printer Setup Procedure, 3.4-3 SERVICE AND REPAIR PROCEDURES, 4.1-1 4.1 GUIDELINES, 4.1-1 4.2 ACCESSING THE HIDDEN SERVICE MENU ITEMS, 4.2-1 Accessing Service Report, 4.2-1 Purpose, 4.2-1 Procedure, 4.
CONTENTS 4.9 DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS, 4.9-1 Tools/Supplies Needed, 4.9-1 Removal, 4.9-1 Installation, 4.9-2 Verification, 4.9-2 4.10 MEMBRANE KEYPAD AND DISPLAY, 4.10-1 Tools/Supplies Needed, 4.10-1 Removal, 4.10-1 Installation, 4.10-2 Verification, 4.10-2 4.11 AC POWER/VACUUM RELAY CARD, 4.11-1 Tools/Supplies Needed, 4.11-1 Removal, 4.11-1 Installation, 4.11-1 Verification, 4.11-2 4.12 +24 VOLT SWITCHING POWER SUPPLY, 4.12-1 Tools/Supplies Needed, 4.12-1 Removal, 4.
CONTENTS Installation, 4.17-2 Verification, 4.17-3 4.18 PROBE MOTOR, 4.18-1 Tools/Supplies Needed, 4.18-1 Removal, 4.18-1 Installation, 4.18-1 Verification, 4.18-1 4.19 TRAVERSE DRIVE BELT, 4.19-1 Tools/Supplies Needed, 4.19-1 Removal, 4.19-1 Installation, 4.19-2 Verification, 4.19-3 4.20 TRAVERSE MOTOR, 4.20-1 Tools/Supplies Needed, 4.20-1 Removal, 4.20-1 Installation, 4.20-2 Verification, 4.20-3 4.21 SYRINGE ASSEMBLY, 4.21-1 Tools/Supplies Needed, 4.21-1 Removal, 4.21-1 Installation, 4.
CONTENTS 4.26 SWEEP-FLOW TUBING, 4.26-1 Tools/Supplies Needed, 4.26-1 Removal, 4.26-1 Installation, 4.26-2 Verification, 4.26-2 4.27 LATEX GAIN ADJUSTMENT, 4.27-1 Tools/Supplies Needed, 4.27-1 Procedure, 4.27-1 4.28 AIM ADJUSTMENT, 4.28-1 Tools/Supplies Needed, 4.28-1 Procedure, 4.28-1 Optional Procedures, 4.28-1 Option 1, 4.28-1 Option 2, 4.28-2 4.29 HGB PREAMP ADJUSTMENT, 4.29-1 Tools/Supplies Needed, 4.29-1 Procedure, 4.29-1 4.30 VACUUM ADJUSTMENT, 4.30-1 Procedure, 4.30-1 4.
CONTENTS 8 A x 7.2 GENERATING A SERVICE REPORT, 7.2-1 The Screen Display, 7.2-1 The Printed Report, 7.2-1 AIM Criteria Results, 7.2-2 Hgb Data, 7.2-3 Sample Results - DO NOT REPORT, 7.2-3 7.3 SERVICE DIAGNOSTIC, 7.3-1 7.4 TROUBLESHOOTING ERROR MESSAGES, 7.4-1 7.5 TROUBLESHOOTING POWER-UP PROBLEMS, 7.5-1 PARTS LISTS, 8.1-1 8.1 MASTER PARTS LISTS, 8.1-1 8.2 ILLUSTRATED PARTS, 8.2-1 QUICK REFERENCE INFORMATION, A.1-1 A.1 TOLERANCES, VOLTAGES AND LIMITS, A.1-1 Limits, A.1-1 Adjustments, A.
CONTENTS B PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE, B.1-1 B.1 C D MESSAGE/ERROR CODE LISTINGS, C.1-1 C.1 INSTRUMENT NON-FATAL ERROR MESSAGES, C.1-1 C.2 INSTRUMENT FATAL ERROR MESSAGES, C.2-1 C.3 AMIBIOS BEEP CODES, C.3-1 OPTIONAL PRINTERS, D.1-1 D.1 E PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE, B.1-1 EPSON TM-290P SLIP PRINTER, D.1-1 Specifications, D.1-1 Operator Controls and LEDs, D.1-1 DIP Switch SW1 Settings, D.1-2 Installation Procedure, D.1-2 Printer Self-Test, D.
C CONTENTS ILLUSTRATIONS 2.1-1 2.1-2 2.2-1 2.2-2 2.2-3 2.2-4 2.3-1 2.3-2 2.3-3 2.3-4 2.3-5 2.4-1 2.4-2 2.4-3 2.4-4 2.5-1 2.5-2 2.5-3 2.5-4 3.1-1 3.2-1 3.2-2 3.2-3 3.4-1 3.4-2 3.4-3 3.4-4 4.3-1 4.4-1 4.6-1 4.6-2 4.8-1 4.9-1 4.10-1 4.12-1 4.13-1 4.13-2 4.14-1 4.15-1 4.16-1 4.16-2 4.17-1 4.19-1 4.20-1 4.21-1 4.22-1 4.24-1 4.26-1 xii Functional Diagram, 2.1-1 Software Menu Tree, 2.1-3 Top View into Upper Chassis, 2.2-3 Top View into Lower Chassis, 2.2-4 AC Power/Vacuum Relay Card, 2.
CONTENTS 7.2-1 7.2-2 7.2-3 7.5-1 7.5-2 8.2-1 8.2-2 8.2-3 8.2-4 8.2-5 8.2-6 8.2-7 8.2-8 8.2-9 A.3-1 A.5-1 D.1-1 PN 4237242C Sample Screen Display, 7.2-1 Sample Printed Report, 7.2-2 Hgb Results Analysis, 7.2-4 Power-Up Troubleshooting Flowchart (part 1 of 2), 7.5-1 Power-Up Troubleshooting Flowchart (part 2 of 2), 7.5-2 MD II, Lower Chassis, Left Side View (See Table 8.2-1), 8.2-1 MD II, Front View (See Table 8.2-2), 8.2-2 MD II, Top View into Lower Chassis (See Table 8.2-3), 8.
C CONTENTS TABLES 2.1-1 2.1-2 2.1-3 2.2-1 2.2-2 2.2-3 2.3-1 2.3-2 2.3-3 2.3-4 2.4-1 2.4-2 2.4-3 2.4-4 2.4-5 2.4-6 2.4-7 2.4-8 2.5-1 2.5-2 2.5-3 2.5-4 2.5-5 2.6-1 3.1-1 3.1-2 6.1-1 6.1-2 7.1-1 7.1-2 7.3-1 7.4-1 8.1-1 8.1-2 8.1-3 8.1-4 8.1-5 8.2-1 8.2-2 8.2-3 8.2-4 8.2-5 8.2-6 8.2-7 8.2-8 8.2-9 A.1-1 A.1-2 xiv Functions the Diluter Table Can Use, 2.1-4 Aspiration Cycle, 2.1-5 Power-Up Cycle, 2.1-6 AT Power Supply Output Connectors, 2.2-1 AC Power/Vacuum Relay Card Connectors and Line Input Ranges, 2.
CONTENTS A.1-3 A.2-1 A.2-2 A.2-3 A.2-4 A.2-5 A.2-6 A.2-7 A.2-8 A.2-9 A.2-10 A.4-1 C.1-1 C.2-1 C.3-1 D.1-1 D.1-2 PN 4237242C Adjustments, A.1-1 AC Power/Vacuum Relay Card Connectors and Line Input Ranges, A.2-1 AT Motherboard Switch and Jumper Settings, A.2-1 DRA Card Jumper Settings, A.2-2 Linear Power Supply Card Jumper Settings, A.2-2 Linear Power Supply Card Test Points, A.2-2 Motor/Solenoid Driver Card Jumper Settings, A.2-3 Motor/Solenoid Driver Card Test Points, A.
CONTENTS xvi PN 4237242C
C CONTENTS 1 PN 4237242C INTRODUCTION, 1.1-1 1.1 MANUAL DESCRIPTION, 1.1-1 Scope, 1.1-1 Organization, 1.1-1 Numbering Format, 1.1-1 Special Headings, 1.1-1 WARNING, 1.1-1 CAUTION, 1.1-2 IMPORTANT, 1.1-2 ATTENTION, 1.1-2 Note, 1.1-2 Conventions, 1.1-2 1.2 SAFETY PRECAUTIONS, 1.2-1 Electronic, 1.2-1 Biological, 1.2-1 Troubleshooting, 1.
CONTENTS 1-ii PN 4237242C
C 1INTRODUCTION 1 1.1 MANUAL DESCRIPTION Scope This manual provides the reference information and procedures necessary for servicing and maintaining the COULTER® MD II™ Series analyzer. This manual is to be used in conjunction with the appropriate customer documents and does not contain information and procedures already covered in those documents.
C INTRODUCTION MANUAL DESCRIPTION CAUTION A CAUTION indicates a situation or procedure that, if ignored, can cause damage to equipment. The word CAUTION is boldfaced in the printed manual and is red in the online manual. IMPORTANT An IMPORTANT indicates a situation or procedure that, if ignored can result in erroneous test results. The word IMPORTANT is boldfaced in the printed manual and is red in the online manual.
C INTRODUCTION SAFETY PRECAUTIONS 1.2 SAFETY PRECAUTIONS Electronic WARNING Risk of personal injury. Rings or other jewelry can contact exposed electronic components causing personal injury from electronic shock. Remove rings and other metal jewelry before performing maintenance or service on the electronic components of the instrument. CAUTION Risk of damage to electronic components.
C INTRODUCTION SAFETY PRECAUTIONS Troubleshooting Bring the following warning to the customer's attention before advising the customer to perform any maintenance, troubleshooting, or service procedures on their instrument. WARNING Risk of personal injury or contamination. If operators do not properly shield themselves while performing service, maintenance and troubleshooting procedures, residual fluids in the instrument could injure or contaminate them.
C CONTENTS 2 PN 4237242C 2 INSTRUMENT DESCRIPTION, 2.1-1 2.1 SYSTEM OVERVIEW, 2.1-1 Description, 2.1-1 Software Menu System, 2.1-2 Software Tables, 2.1-2 Aspirate Table, 2.1-5 Power-up Table, 2.1-6 2.2 POWER SUPPLY, 2.2-1 Overview, 2.2-1 AT Power Supply, 2.2-1 Inputs, 2.2-1 Outputs, 2.2-1 Switches, 2.2-2 AC Power/Vacuum Relay Card, 2.2-2 Inputs, 2.2-5 Outputs, 2.2-5 Test Points, 2.2-5 Jumpers, 2.2-5 +24 Volt Switching Power Supply, 2.2-6 Inputs, 2.2-6 Outputs, 2.2-6 Adjustments, 2.
CONTENTS Diagnostic Data Acquisition Circuit, 2.4-4 Utility Timer Circuit, 2.4-4 Support Circuits, 2.4-4 Inputs, 2.4-5 Outputs, 2.4-5 Adjustments, 2.4-5 Switches and Jumpers, 2.4-6 Diluter Resource Adapter (DRA) Card, 2.4-6 Motor Controller, 2.4-6 Solenoid Controller, 2.4-7 Support Circuitry, 2.4-7 Inputs, 2.4-7 Outputs, 2.4-7 Jumpers, 2.4-8Motor/Solenoid Driver Card, 2.4-8 Motor Driver, 2.4-9 Solenoid Driver, 2.4-9 Sensor Control, 2.4-9 +24 V POWER ON Signal, 2.4-9 +24 V POWERFAIL (PF/PG) Signal, 2.
CONTENTS Outputs, 2.5-9 Jumper, 2.5-10 Adjustments, 2.5-10 2.6 PERIPHERALS, 2.6-1 Floppy Disk Drive, 2.6-1 Display, 2.6-1 Keypad, 2.6-1 Rear Panel Interface Connectors, 2.6-1 ILLUSTRATIONS 2.1-1 2.1-2 Functional Diagram, 2.1-1 Software Menu Tree, 2.1-3 2.2-1 2.2-2 2.2-3 2.2-4 Top View into Upper Chassis, 2.2-3 Top View into Lower Chassis, 2.2-4 AC Power/Vacuum Relay Card, 2.2-5 Linear Power Supply Card, 2.2-7 2.3-1 2.3-2 2.3-3 2.3-4 2.3-5 Fluidics Panel Components, 2.3-1 Diluter Panel, 2.
CONTENTS 2-iv 2.4-1 2.4-2 2.4-3 2.4-4 2.4-5 2.4-6 2.4-7 2.4-8 AT Motherboard Switch and Jumper Settings, 2.4-1 URA Card Switch and Jumper Settings, 2.4-6 DRA Card Output Connectors, 2.4-7 DRA Card Jumper Settings, 2.4-8 Motor/Solenoid Driver Card Input Connections, 2.4-10 Motor/Solenoid Driver Card Output Connections, 2.4-10 Motor/Solenoid Driver Card Test Points, 2.4-11 Motor/Solenoid Driver Card Jumper Settings, 2.4-11 2.5-1 2.5-2 2.5-3 2.5-4 2.5-5 Sensor Preamp Adapter Card Input Connections, 2.
C 2INSTRUMENT DESCRIPTION 2 2.1 SYSTEM OVERVIEW Description The MD II instrument is an automated hematology analyzer and leukocyte differential counter For In Vitro Diagnostic Use in clinical laboratories. It is contained in one unit, with additional space needed only for the external Printer(s), the reagents, and if used, a waste container.
C INSTRUMENT DESCRIPTION SYSTEM OVERVIEW The MD II cycle begins with the presentation of a whole-blood sample to a self-washing aspirate probe. The MD II then aspirates 12 µL of blood, dilutes and analyzes the sample and makes the results available to a Liquid Crystal Display (LCD) and Printer. Instrument intelligence is provided by a program loaded at power ON from a diskette.
C INSTRUMENT DESCRIPTION SYSTEM OVERVIEW Figure 2.1-2 Software Menu Tree 1. RUN SAMPLE ENTER ID ENTER RANGE PASSWORD CONTROL LEVEL OPTIONS 1. LOW 2. NORMAL 3. HIGH 2. RUN CONTROL 3. STARTUP ASPIRATE RUN CONTROL 4. REVIEW LAST SAMPLE 1. SETUP 1. DATE/TIME 2. IQAP ID # 1. SELECT DATE FORMAT 2. CHANGE DATE AND TIME 3. HOST SETTINGS 1. HOST COMMUNICATION 2. BAUD RATE 3. DATA BITS 4. PARITY 5. STOP BITS 4. OPTIONS 1. PRINT 1. AUTOMATIC 2. MANUAL 2. CONTROL 1. COULTER CONTROL/AUTOMATED DISK 2.
C INSTRUMENT DESCRIPTION SYSTEM OVERVIEW The MD II has seven diluter tables or cycles. These are the aspirate, power up, startup, shutdown, prime, latex and clean bath tables. Chapter 6 has timing charts for all the diluter tables. Because they are the most useful in describing and troubleshooting instrument operation, the aspirate cycle and the power-up cycle are described in detail. Make sure that you use the most recent timing chart when troubleshooting timing concerns. Table 2.
C INSTRUMENT DESCRIPTION SYSTEM OVERVIEW Aspirate Table The aspirate cycle, Table 2.1-2, is the most important table for the instrument. It is used for sample analysis during a sample, control, calibration, and reproducibility run. Table 2.1-2 Aspiration Cycle Time (seconds) Activity Requested 0.0 Move aspirate probe up and turn on vacuum pump. 1.0 Read Hgb Blank #2, move aspirate probe to aspirate position, drain WBC bath and move aspirate syringes down. 2.
C INSTRUMENT DESCRIPTION SYSTEM OVERVIEW Power-up Table The power-up table, Table 2.1-3, is run by the instrument during its power-up process. Table 2.1-3 Power-Up Cycle Time (seconds) Activity Requested 0.0 Drain WBC bath. Rinse and drain 3 times consecutively. This uses about 16 mL of diluent from reservoir. 14.0 Check diluent sensor. It should not see diluent and displays ERROR DETECTED (017) Unable to Sense Diluent Level if it does. Drain VIC and fill diluent reservoir to sensor. 80.
C INSTRUMENT DESCRIPTION POWER SUPPLY 2.2 POWER SUPPLY Overview Ac is input into the lower chassis using a connector that is also a line filter. Both lines are fused, F1 on the hot line and F2 on the neutral line. For 120 Vac units, a 4.0-A SLO-BLO fuse is used, and for 220 Vac units a 2.0-A SLO-BLO fuse is used.
C INSTRUMENT DESCRIPTION POWER SUPPLY Switches r Power On/Off switch - used as instrument’s Power ON/OFF switch r Ac Select switch - selects 115 or 230 Vac supply (not accessible when in unit) AC Power/Vacuum Relay Card The AC Power/Vacuum Relay card is found in the lower chassis (Figure 2.2-2) and takes the ac input and distributes it. The card receives ac directly from line input and directs it for system use. Ac into the card is first sent through relay K2.
C INSTRUMENT DESCRIPTION POWER SUPPLY Figure 2.2-1 Top View into Upper Chassis AT POWER SUPPLY SCREWS COVER SCREWS AC POWER CABLE DRA CARDS SPAD CARD J15 SER1 AT POWER SUPPLY P8 J17 FLOPPY J18 PARALLEL J14 SER2 URA CARD AT MOTHERBOARD P9 SW1 J20 IDE J19 EXT BATT FLOPPY DISK DRIVE BATTERY BANK 0 BANK 1 BANK 2 BANK 3 W3 7 4 1 _ MEMBRANE KEYPAD/DISPLAY PN 4237242C 8 5 2 0 9 6 3 . 7242018A 2.
C INSTRUMENT DESCRIPTION POWER SUPPLY Figure 2.2-2 Top View into Lower Chassis 90° POSITION 45° POSITION RETAINING SCREW +24 V POWER SUPPLY MOTOR/SOLENOID DRIVER CARD LINEAR POWER SUPPLY CARD AC POWER/ VACUUM RELAY CARD -V4 + -V3 + -2V + +S -S ON/OFF PF/PG A C P O W E R /V A C U U M V1 + RTN RTN + FLEX CONNECT CARD VACUUM SENSOR CARD MOTOR/SOLENOID DRIVER CARD VACUUM PUMP SENSOR PREAMP ADAPTER CARD SOLENOID INTERCONNECT CARD 2.
C INSTRUMENT DESCRIPTION POWER SUPPLY Figure 2.
C INSTRUMENT DESCRIPTION POWER SUPPLY Table 2.2-2 AC Power/Vacuum Relay Card Connectors and Line Input Ranges Connector Line Input Range 100 VOLTS 90 - 110 Vac 120 VOLTS 110 - 132 Vac 220 VOLTS 198 - 242 Vac 240 VOLTS 220 - 264 Vac There are four plugs available to select the appropriate range. Though units are sold for a specific power range, the other three plugs are available if the power does not match with the plug being used.
C INSTRUMENT DESCRIPTION POWER SUPPLY The white/green wires connected to J1-1 and J1-4 supply -18.5 Vrms to bridge rectifier VR3. This produces an unregulated -25 Vdc that is regulated using an LM7915CK (U6) regulator circuit. The output of this regulator circuit is the Linear Power Supply card -15 Vdc output. The red wire pair connected through J1-3 and J1-6 provide 165 Vrms for the aperture supply voltages. Bridge rectifier VR1 produces about +240 Vdc from this supply.
C INSTRUMENT DESCRIPTION POWER SUPPLY Inputs r J1 - Transformer ac input to Linear Power Supply card r J3 - commands, +5 V from SPAD card Outputs r J2 - Hgb LED r J4 - ±15 Vdc r J5 - RBC aperture voltage, WBC aperture voltage, and zap aperture voltage Test Points Table 2.
C INSTRUMENT DESCRIPTION FLUIDICS PANEL 2.3 FLUIDICS PANEL Overview The Fluidics Panel is responsible for receiving, preparing and presenting the sample for electronic analysis, and preparing for introduction of the next sample. Whole-blood sample is aspirated through a sample aspirate probe, then transported to the counting chambers. This is accomplished by the Probe/Wipe Traverse Assembly. A self-washing mechanism is built into this assembly, relieving the operator of having to wipe the probe.
INSTRUMENT DESCRIPTION FLUIDICS PANEL Diluter Panel The Diluter Panel is located in the lower front of the instrument and is the main fluidic module of the MD II. It holds all the fluid solenoid valves, the VIC, the sweep-flow tubing, the aperture and bath components, and the Hgb LED and detector. See Figure 2.3-2. The Sensor Preamp Adapter card is also attached to the back of the Diluter Panel through the Aperture Electrode module cable shield.
INSTRUMENT DESCRIPTION FLUIDICS PANEL Figure 2.3-2 Diluter Panel 15 16 1 14 2 3 13 4 12 5 11 6 10 9 1. 2. 3. 4. 5. 6. 7. 8. WBC BATH/APERTURE VACUUM ISOLATOR CHAMBER RBC VACUUM VALVE (LV5) RBC BATH/APERTURE Hgb LED RINSE VALVE (LV11) DRAIN VALVE (LV7) PREFILL VALVE (LV13) 8 7 9. 10. 11. 12. 13. 14. 15. 16.
INSTRUMENT DESCRIPTION FLUIDICS PANEL Solenoids provide the fluidic logic for the MD II. There are 12 fluidic solenoids mounted on the Diluter Panel (Figure 2.3-2). Table 2.3-1 lists these solenoids, their name, type and function. Table 2.3-1 Diluter Panel Solenoids and Their Function Solenoid Name Type Function LV3 Vac Select 3-way Selects regulated or raw pump vacuum for the VIC. LV4 Sweep Flow 2-way Opens the diluent path for sweep flow.
INSTRUMENT DESCRIPTION FLUIDICS PANEL Figure 2.3-3 Probe-Wipe Traverse Assembly PROBE/ OPTO SENSOR CARD PROBE MOTOR TRAVERSE MOTOR TRAVERSE HOUSING SPRING BELT TENSIONER SAMPLE ASPIRATE PROBE 7242006A Peristaltic Pump Assembly The Peristaltic Pump Assembly is located on the lower left side of the instrument and consists of a plate holding four peristaltic pumps and their associated tubing. See Figure 2.3-4. Table 2.3-2 gives each pump’s location and function.
INSTRUMENT DESCRIPTION FLUIDICS PANEL Table 2.3-2 Peristaltic Pumps Location and Function Pump Location Function PM1 (Air/Mix) Upper right Provides air for isolation and mixing bubbles. PM2 (Diluent) Upper left Fills the diluent reservoir from the diluent tube. PM3 (Rinse) Lower left Rinses the baths with fluid from the diluent reservoir. PM4 (Waste) Lower right Drains the VIC and both red and white baths.
INSTRUMENT DESCRIPTION FLUIDICS PANEL Individual Components Mounted to Fluidics Panel The following individual components are mounted directly on the Fluidics Panel: r Cycle counter and air solenoids r Lyse pump (PM5 r Diluent reservoir r Solenoid Interconnect card r Flex Connect card r Vacuum regulator. Cycle Counter and Air Solenoids The front left portion of the lower chassis houses the cycle counter, solenoids LV9 and LV12, and the lyse pump PM5 (Figure 2.3-1).
INSTRUMENT DESCRIPTION FLUIDICS PANEL Lyse Pump (PM5) The lyse pump (Figure 2.3-1) is a solenoid pump with a spring return. Several specifications of this pump should be considered when troubleshooting the lyse system. It has an adjustable working range of 200 to 450 µL and should be set to 415 ±5 µL. It cannot be energized for more than 6 seconds or damage will result. The MD II energizes the lyse pump for 300 ms whenever it dispenses lyse and has a fail-safe that cuts power after 1 second.
INSTRUMENT DESCRIPTION FLUIDICS PANEL Vacuum Regulator The vacuum regulator (Figure 2.3-1) is a solid-state regulator used to regulate the 6-in. Hg count vacuum. It is located in the upper right area of the front panel and is accessible to customers for adjustment. PN 4237242C 2.
INSTRUMENT DESCRIPTION FLUIDICS PANEL 2.
C INSTRUMENT DESCRIPTION SYSTEM CONTROL 2.4 SYSTEM CONTROL System control is the heart of the instrument. The components that form system control translate executing programs (software) into mechanical actions. The cards responsible for system control are the: AT motherboard, URA, DRAs and Motor/Solenoid Driver. AT Motherboard The motherboard is located in the upper chassis (Figure 2.2-1) and is the primary control in the system. It is a standard AT motherboard, using an 80386, 20-MHz CPU.
INSTRUMENT DESCRIPTION SYSTEM CONTROL Figure 2.4-1 AT Motherboard J15 (Serial 1 port) J15 SER1 J14 (Serial 2 port) P8 J18 (Parallel printer port) J17 FLOPPY J18 PARALLEL J14 SER2 GROUND SCREW P9 J17 (Floppy disk drive) SW1 J19 EXT BATT J20 IDE SW1 BATTERY CC MD V1.
INSTRUMENT DESCRIPTION SYSTEM CONTROL Keypad Controller The heart of the keypad controller is an 8279 keyboard controller chip. This chip is operated in "scan keyboard" mode. The keypad is a standard row and column matrix, and with the help of an HC138 decoder used to decode the rows, the keypad controller is able to sense any pressed keys. Communication with the keypad is through J5 (Figure 2.4-2), a 20-pin in-line connector that receives the flex cable connector from the keypad.
C INSTRUMENT DESCRIPTION SYSTEM CONTROL Diagnostic Data Acquisition Circuit The Diagnostic Data Acquisition circuit receives voltage data from the Vacuum Sensor, Hgb Preamp and Sensor Preamp Adapter cards and converts this for use by the instrument software. The supplies are input to an HI508a analog multiplexer. The CPU selects which input to the multiplexer is output to an AD7572 A/D converter.
C INSTRUMENT DESCRIPTION SYSTEM CONTROL Inputs r J1 - POWER r J2 - PVAC (VACUUM SENSOR signal) r J3 - AIM (from SPAD) r J5 - KEYBOARD r J6 - Hgb preamp r J7 - PREAMP DIAG r P1, P2 - AT bus edge connector Outputs r J4 - Display r P1, P2 - AT bus edge connector Adjustments r R5 - Display contrast r R6 - A/D converter zero adjustment r R7 - A/D converter scale adjustment Figure 2.
INSTRUMENT DESCRIPTION SYSTEM CONTROL Switches and Jumpers Table 2.
INSTRUMENT DESCRIPTION SYSTEM CONTROL Commands to the GAL are provided by an 8-bit latch. Seven bits of this latch represent the parameters SENSOR MODE, STOP, ENABLE, SENSOR ENABLE, DIRECTION, STEP and POWER. The eighth bit does not go to the GAL. It is passed through a buffer to the circuit connector and is used to control one solenoid. The output of the latch is also attached to a unidirectional bus buffer. By enabling this buffer, the host CPU can read the command latch.
C INSTRUMENT DESCRIPTION SYSTEM CONTROL Jumpers Table 2.4-4 DRA Card Jumper Settings Jumper Description MD II Setting X4 Sets card for IRQ11, needed for DRA1 1-3 Sets card for IRQ12, needed for DRA2 2-3 Sets card to DRA1 or DRA2 ON - DRA1 X5 OFF- DRA2 X6 Connects oscillator to circuit when jumped ON Figure 2.
C INSTRUMENT DESCRIPTION SYSTEM CONTROL Motor Driver All motors use the same Motor Driver circuit, which uses a UDN2878W driver device. Four phase signals are input from the DRA card(s). The driver outputs four +24 V lines to the motor in the phases set by the DRA card(s). An Overload Timer circuit on the Motor/Solenoid Driver card offers protection to the motors and drivers. The +24 V, which is received by the Motor Driver circuit’s 25-W resistors, is delivered through Q3, a 10-A transistor.
INSTRUMENT DESCRIPTION SYSTEM CONTROL At power up, the programmable logic device is reset, which clears all latches. This causes Q4 to conduct, grounding the POWER ON signal. The system remains in this state with the +24 V off until the instrument’s software is loaded and turns on the +24 V. +24 V POWERFAIL (PF/PG) Signal The +24 V power supply outputs a POWERFAIL signal.
C INSTRUMENT DESCRIPTION SYSTEM CONTROL Test Points Table 2.
C INSTRUMENT DESCRIPTION SYSTEM CONTROL Figure 2.4-4 Motor/Solenoid Driver Card J4 (To/from DRA1, J1) J18 (Resistor bank) J5 (To/from DRA1, J2) J2 (To/from DRA2, J2) J23 (Resistor bank) J15 (To +24 V POWER ON) J1 (To/from DRA2, J1) X1 (ON) J6 (To/from DRA1, J3) J3 (To/from DRA2, J3) J11 (To/from Flex Connect card) J8 (To peristaltic pump motors) J10 (To/from Syringe Assembly/ traverse motor J7 (To Solenoid Interconnect card) J12 (Not used) MOTOR/SOLENOID DRIVER CARD X2 (ON) 7242012A 2.
C INSTRUMENT DESCRIPTION DATA ACQUISITION 2.5 DATA ACQUISITION The data acquisition section is responsible for the accumulation and measurement of system data that is used to determine final system output (instrument results). The data acquisition section includes the: r r r r Sensor Preamp Adapter (SPA) card Hgb Preamp card Vacuum Sensor card SPAD card. Sensor Preamp Adapter (SPA) Card The Sensor Preamp Adapter card is located in the lower chassis (Figure 2.
INSTRUMENT DESCRIPTION DATA ACQUISITION Outputs Table 2.5-2 Sensor Preamp Adapter Card Output Connections Connector Description J3-J6 Analog power distribution (out) J9 WBC signal J11 RBC signal J12 PLT signal Adjustments There are four adjustable resistors on this card. They are adjusted at the factory to set dc levels in the Preamp and Dc Restorer stages. The adjustment requires a test setup that is impractical for field procedures. Jumpers Table 2.
INSTRUMENT DESCRIPTION DATA ACQUISITION Figure 2.
INSTRUMENT DESCRIPTION DATA ACQUISITION dark current from the detector. There is interaction between these two adjustments, so they must be adjusted together.
INSTRUMENT DESCRIPTION DATA ACQUISITION The vacuum measurement is the difference between these two reference readings. During a sample analysis cycle, one zero reference is taken prior to the first count period, and a high reference voltage reading is taken for each of the 12 count periods. This produces a vacuum measurement for each of the count periods. Figure 2.5-2 Vacuum Sensor Card Block Diagram 8.
INSTRUMENT DESCRIPTION DATA ACQUISITION Test Points Table 2.5-4 Vacuum Sensor Card Test Points Test Point Description TP1 8.006 V reference voltage (7.964 V to 8.049 V) TP2 Transducer output TP3 Gain adjustment output TP4 Ground TP5 VAC OUT Figure 2.5-3 Vacuum Sensor Card TEST POINTS (1-5) J3 (To solenoid LV2) J2 (To solenoid LV1) J1 (+ 15 V in VAC OUT) TP1 7242014A Sensor Processing Adapter with Diagnostics (SPAD) Card The SPAD card is located in the upper chassis (Figure 2.2-1).
INSTRUMENT DESCRIPTION DATA ACQUISITION The SPAD’s input connections are summarized in Table 2.5-5 and their location is shown on Figure 2.5-4. Figure 2.5-4 also shows the location and setting of the jumper. Table 2.5-5 and Figure 2.5-4 are at the end of the description of the SPAD card. The card is comprised of the following circuitry. Power Supply Conditioning This card receives +5 V from the +24 V power supply through the AT bus slot and ±15 V from the Linear Power Supply card through connector J4.
INSTRUMENT DESCRIPTION DATA ACQUISITION Octal Digital-to-Analog Converter (DAC) The octal DAC contains eight identical DACs. They are attached to a common reference but are individually latched. They provide: r r r r r RBC aperture current voltage setting (coarse and fine) WBC aperture current voltage setting (coarse and fine) RBC counting threshold WBC counting threshold Plt lower and upper thresholds. Aperture Signal Processing The signal processing functions are programmed into another EPLD.
INSTRUMENT DESCRIPTION DATA ACQUISITION Test Pulse Generator A test pulse generator has been established on the SPAD card with two ICs. U40 is a logic chip that establishes the pulses and U38 is the A/D converter used by the logic chip. The MD II uses this test pulse generator to create a RAMP pulse train during the PULSE TEST accessed from the Service menu. The SPAD card processes this pulse train and generates a report. If Auto Print is active, histograms are also displayed.
INSTRUMENT DESCRIPTION DATA ACQUISITION Figure 2.5-4 SPAD Card X7 (ON) J9 (AIM to URA) J8 (10-pin power control) J4 (+15 V in) J3 (WBC PREAMP signal in) W R J2 (RBC PREAMP signal in) P J1 (PLT PREAMP signal in) P2 (AT bus edge connector) J5 (Not used) P1 (AT bus edge connector) 7242015A Jumper X7 (Set to ON) r ON - Connects oscillator to circuit r OFF- oscillator for card testing Adjustments There are two potentiometers onboard, R85 and R86.
C INSTRUMENT DESCRIPTION PERIPHERALS 2.6 PERIPHERALS Floppy Disk Drive The floppy disk drive is a standard 3.5 in., 1.44 MB, Double-sided High-density drive. The main system program is loaded from this drive on power up or reboot. A program disk is used to power up the instrument, but a calibration or control disk may be requested. Software updates merely require that the new program disk be used at power ON. Display Visual output is provided by a 4-row by 40-column, character only LCD.
INSTRUMENT DESCRIPTION PERIPHERALS 2.
C CONTENTS 3 INSTALLATION PROCEDURES, 3.1-1 PART A: INSTRUMENT INSTALLATION 3.1 PREINSTALLATION CHECKS, 3.1-1 Space and Accessibility Requirements, 3.1-1 Power Requirements, 3.1-1 3.2 INITIAL SETUP, 3.2-1 Unpack the Instrument, 3.2-1 Connect the Reagents, 3.2-2 Connect the Printer, 3.2-3 Enter the Initial Settings, 3.2-4 3.3 INSTRUMENT VERIFICATION, 3.3-1 Startup the Instrument, 3.3-1 Calibrate the Instrument, 3.3-1 PART B: UPGRADE AND OPTION INSTALLATION 3.4 OPTIONAL TICKET PRINTERS, 3.
CONTENTS 3-ii PN 4237242C
C 3INSTALLATION PROCEDURES 3 PART A: INSTRUMENT INSTALLATION 3.1 PREINSTALLATION CHECKS Space and Accessibility Requirements If the reagent pack will be placed on the floor, measure the bench height. The reagent pack should not be more than 36 in. below the lyse pump. This allows for a bench height of about 32 in. Check the site for proper space allocation (Table 3.1-1) . Table 3.1-1 Space Requirements Linear Dimensions Required by Instrument Preferred by Service Depth 19.
C INSTALLATION PROCEDURES PREINSTALLATION CHECKS Table 3.1-2 Connectors’ Part Numbers and Line Input Ranges Connector Range Part Number 100 VOLTS 90 - 110 Vac 6028623-0 120 VOLTS 110 - 132 Vac 6028600-1 220 VOLTS 198 - 242 Vac 6028624-8* 240 VOLTS 220 - 264 Vac 6028625-6* * An assembly, PN 6706318-0, is included with the 230 Vac instrument. It has both the 200 VOLTS and 240 VOLTS connectors. Figure 3.
C INSTALLATION PROCEDURES INITIAL SETUP 3.2 INITIAL SETUP Unpack the Instrument 1. Unpack all boxes. 2. Using the packing list, ensure that no items are missing including the proper reagents, controls and calibrators. 3. Check the instrument for damage. 4. Check that the instrument has caution and compliance labels near the power connector. 5. Measure the line voltage that the MD II will be plugged into. Verify that the instrument version (100 Vac, 120 Vac or 230 Vac) matches this line voltage.
C INSTALLATION PROCEDURES INITIAL SETUP Connect the Reagents The connections for the reagents are at the bottom right corner on the left side of the instrument (Figure 3.2-1). 1. Connect the diluent reagent using blue stripe tubing (PN 3202209-0) to the blue fitting (diluent). Use a Luer fitting (PN 6232503-8). 2. Connect the lytic reagent using EVA tubing (PN 3202221-9) to the white fitting (lyse). Use a Luer fitting (PN 6232503-8).
C INSTALLATION PROCEDURES INITIAL SETUP Connect the Printer 1. 2. Determine which type of Printer(s) need to be installed. r To install the standard Graphic Printer only, follow this procedure. r To install the optional Ticket Printer or the optional Ticket Printer and the Graphic Printer, go to Heading 3.4, OPTIONAL TICKET PRINTERS. Connect the Printer cable between the Printer and the 25-pin parallel connector at the back of the MD II (Figure 3.2-2.). Figure 3.
C INSTALLATION PROCEDURES INITIAL SETUP Figure 3.2-3 CITIZEN GSX-190 Printer settings Enter the Initial Settings 3.2-4 1. Insert a Program Disk in the floppy disk drive and turn the instrument’s power ON. 2. Ensure that the system reaches the Main Menu without detecting any errors. 3. Set all relevant system settings: date, time, print options, host options and reagent information. 4. Set the customer's IQAP # if it is available.
C INSTALLATION PROCEDURES INSTRUMENT VERIFICATION 3.3 INSTRUMENT VERIFICATION Startup the Instrument 1. Prime the reagent and sweep-flow lines using the Cycle Reagents key. 2. Perform the Hgb Preamp and the Vacuum Adjustment procedures (Headings 4.29 and 4.30, respectively). 3. Select 3 STARTUP and verify proper backgrounds. 4. Perform the Latex Gain Adjustment procedure (Heading 4.27). 5. Set the AIM “target values” (Heading 4.28). 6. Run controls and verify proper system operation.
C INSTALLATION PROCEDURES INSTRUMENT VERIFICATION 3.
C INSTALLATION PROCEDURES OPTIONAL TICKET PRINTERS PART B: UPGRADE AND OPTION INSTALLATION 3.4 OPTIONAL TICKET PRINTERS Epson TM-290P Slip Printer Purpose A parallel version of the Epson TM-290P Slip Printer is available for use on the MD II. See Figure 3.4-1. Figure 3.4-1 Epson TM-290P Slip Printer POWER RELEASE PAPER OUT RESET FORWARD REVERSE RELEASE 7303007A This section contains two procedures.
INSTALLATION PROCEDURES OPTIONAL TICKET PRINTERS Figure 3.4-2 DIP Switch, SW1, Location SW 1 ON 1 2 3 4 5 6 7 8 9 10 7303003A CAUTION Risk of damage to equipment. Turning the instrument’s power ON before connecting the Printer to the instrument could damage the instrument. Ensure the power is OFF before connecting the Printer. 3. Ensure the MD II power is off. 4. Connect the Printer as shown in Figure 3.4-3. Connect the power cord last. Figure 3.
INSTALLATION PROCEDURES OPTIONAL TICKET PRINTERS 6. Turn on the MD II and print the calibration factors to verify that the Printer and instrument are working together. Note: The first time the Ticket Printer option is enabled, the instrument will request that you insert the Ticket Key Disk. Once the ticket printer enable is recorded in CMOS, it will not be requested again. Two-Printer Setup Procedure 1. Unpack the Ticket Printer.
INSTALLATION PROCEDURES OPTIONAL TICKET PRINTERS Figure 3.4-4 Printer Connections for Two Printers POWER LINE CORD (AC DILUTER TO ANALYZER) SERIAL PORT 2 SERIAL PORT 1 25-PIN PARALLEL CONNECTOR CENTRONICS PARALLEL PRINTER CABLE A I/O A/B SWITCH BOX B MD II AC POWER CABLE POWER SUPPLY GRAPHIC PRINTER 7. 8. TICKET PRINTER 7242052B If this is the initial installation of the Graphic Printer: a. Load paper into the Graphic Printer. b. Set the Printer’s configuration for MD II use.
C CONTENTS 4 SERVICE AND REPAIR PROCEDURES, 4.1-1 4.1 4.2 GUIDELINES, 4.1-1 ACCESSING THE HIDDEN SERVICE MENU ITEMS, 4.2-1 Accessing Service Report, 4.2-1 Purpose, 4.2-1 Procedure, 4.2-1 Accessing Service Diagnostic, 4.2-1 Purpose, 4.2-1 Procedure, 4.2-1 System Reboot, 4.2-2 4.3 REMOVING THE TOP COVER, 4.3-1 4.4 OPENING THE LOWER CHASSIS, 4.4-1 4.5 AT POWER SUPPLY, 4.5-1 Tools/Supplies Needed, 4.5-1 Removal, 4.5-1 Installation, 4.5-1 Verification, 4.5-2 4.6 AT MOTHERBOARD, 4.
CONTENTS 4.11 AC POWER/VACUUM RELAY CARD, 4.11-1 Tools/Supplies Needed, 4.11-1 Removal, 4.11-1 Installation, 4.11-1 Verification, 4.11-2 4.12 +24 VOLT SWITCHING POWER SUPPLY, 4.12-1 Tools/Supplies Needed, 4.12-1 Removal, 4.12-1 Installation, 4.12-2 Verification, 4.12-3 4.13 LINEAR POWER SUPPLY CARD, 4.13-1 Tools/Supplies Needed, 4.13-1 Removal, 4.13-1 Installation, 4.13-2 Verification, 4.13-3 4.14 VACUUM SENSOR CARD, 4.14-1 Tools/Supplies Needed, 4.14-1 Removal, 4.14-1 Installation, 4.
CONTENTS 4.19 TRAVERSE DRIVE BELT, 4.19-1 Tools/Supplies Needed, 4.19-1 Removal, 4.19-1 Installation, 4.19-2 Verification, 4.19-3 4.20 TRAVERSE MOTOR, 4.20-1 Tools/Supplies Needed, 4.20-1 Removal, 4.20-1 Installation, 4.20-2 Verification, 4.20-3 4.21 SYRINGE ASSEMBLY, 4.21-1 Tools/Supplies Needed, 4.21-1 Removal, 4.21-1 Installation, 4.21-3 Verification, 4.21-4 4.22 PERISTALTIC PUMP MOTOR AND SPOOL, 4.22-1 Tools/Supplies Needed, 4.22-1 Removal, 4.22-1 Installation, 4.22-2 Verification, 4.22-2 4.
CONTENTS 4.28 AIM ADJUSTMENT, 4.28-1 Tools/Supplies Needed, 4.28-1 Procedure, 4.28-1 Optional Procedures, 4.28-1 Option 1, 4.28-1 Option 2, 4.28-2 4.29 HGB PREAMP ADJUSTMENT, 4.29-1 Tools/Supplies Needed, 4.29-1 Procedure, 4.29-1 4.30 VACUUM ADJUSTMENT, 4.30-1 Procedure, 4.30-1 4.31 LYSE VOLUME ADJUSTMENT/VERIFICATION, 4.31-1 Tools/Supplies Needed, 4.31-1 Procedure, 4.31-1 ILLUSTRATIONS 4.3-1 Top View into Upper Chassis, 4.3-1 4.4-1 Top View into Lower Chassis, 4.4-1 4.6-1 4.
CONTENTS 4.21-1 Syringe Assembly, 4.21-2 4.22-1 Peristaltic Pump Motors, 4.22-1 4.24-1 Aperture Bath Assembly, 4.24-1 4.26-1 Sweep-Flow Tubing, 4.
CONTENTS 4-vi PN 4237242C
C 4SERVICE AND REPAIR PROCEDURES 4 4.1 GUIDELINES PN 4237242C r All the tools and supplies you need to perform a specific procedure are listed under Tools/Supplies Needed, at the beginning of the applicable procedure. r Each time you are told to turn the instrument’s power ON, you should have the Program Disk in the floppy disk drive.
SERVICE AND REPAIR PROCEDURES GUIDELINES 4.
C SERVICE AND REPAIR PROCEDURES ACCESSING THE HIDDEN SERVICE MENU ITEMS 4.2 ACCESSING THE HIDDEN SERVICE MENU ITEMS There are three service functions hidden (they do not show up on the display) from the customer. They are: r Service Report r Service Diagnostic r System Reboot. Accessing Service Report Purpose Access the Service Report to obtain data about the previous aspirate cycle, whether it was a sample, a control or a calibrator.
SERVICE AND REPAIR PROCEDURES ACCESSING THE HIDDEN SERVICE MENU ITEMS System Reboot 4.2-2 1. Insert the Program Disk. 2. From the Main Menu, select 7 [no menu item displays on the screen].
C SERVICE AND REPAIR PROCEDURES REMOVING THE TOP COVER 4.3 REMOVING THE TOP COVER 1. At the rear of the unit, unscrew the three, #2 Phillips-head screws, Figure 4.3-1. 2. Slide the cover off toward the front of the instrument. Figure 4.
SERVICE AND REPAIR PROCEDURES REMOVING THE TOP COVER 4.
A SERVICE AND REPAIR PROCEDURES OPENING THE LOWER CHASSIS 4.4 OPENING THE LOWER CHASSIS 1. Open the front door of the instrument. 2. Loosen the captive #2 Phillips-head retaining screw just above the traverse motor (Figure 4.4-1). 3. Swing the upper chassis up and back and lock the support arms in place. Figure 4.
SERVICE AND REPAIR PROCEDURES OPENING THE LOWER CHASSIS 4.
C SERVICE AND REPAIR PROCEDURES AT POWER SUPPLY 4.5 AT POWER SUPPLY Tools/Supplies Needed B #2 Phillips-head screwdriver B Voltmeter Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable from the rear of the power supply (Figure 4.3-1). 3. Remove the top cover as directed under Heading 4.3. 4. Remove electrical connections to the power supply: r P1 connects to the floppy disk drive power connector.
SERVICE AND REPAIR PROCEDURES AT POWER SUPPLY 4. 5. Connect the power supply to the instrument: r P1 connects to the floppy disk drive power connector. r P2 connects to a cable from the lower chassis terminating at the AC Power/Vacuum Relay card. r P4 and P5 attach to the AT motherboard connectors P8 and P9. Reconnect the ac power cable and turn ON the instrument’s power. Wait until the instrument reaches the Main Menu.
C SERVICE AND REPAIR PROCEDURES AT MOTHERBOARD 4.6 AT MOTHERBOARD Tools/Supplies Needed B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable from the rear of the power supply (Figure 4.3-1). 3. Remove the top cover as directed under Heading 4.3. 4. Remove the SPAD card (Heading 4.7), the URA card (Heading 4.8) and the two DRA cards (Heading 4.9) shown in Figure 4.6-1. 5.
SERVICE AND REPAIR PROCEDURES AT MOTHERBOARD 6. Disconnect the floppy disk drive, the external interface cables and the speaker: r Floppy disk drive connects to J17. r Serial 1 port connects to J15. r Serial 2 port connects to J14. r Parallel (Printer) port connects to J18. r Speaker port connects to J22. 7. Remove the two motherboard ground screws (Figure 4.6-2). 8. Remove the nylon retaining posts (Figure 4.6-1): a. Start at one end and work to the other. b.
SERVICE AND REPAIR PROCEDURES AT MOTHERBOARD Verification Without a keyboard and monitor the best way to verify AT motherboard operation is to use the instrument. 1. Turn ON the instrument’s power and check to see that the system reaches the Main Menu. This tells you that you have successfully: r Passed BIOS POST r Booted r Read from the floppy disk drive r Executed a program r Communicated with all four cards plugged into the bus. Figure 4.
SERVICE AND REPAIR PROCEDURES AT MOTHERBOARD 4.6-4 2. Verify the ability to write to the floppy disk drive by temporarily changing a configuration item, such as a host or Printer option. This forces the system to write the new configuration to disk. If no problem is observed, restore the system to its original configuration. 3. Verify the operation of the Printer port by printing a configuration item such as the calibration factors. 4.
C SERVICE AND REPAIR PROCEDURES SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD 4.7 SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD Tools/Supplies Needed B #1 Phillips-head screwdriver B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Remove the top cover as directed under Heading 4.3. 3. Locate the SPAD card (Figure 4.3-1). 4.
SERVICE AND REPAIR PROCEDURES SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD Verification 4.7-2 1. From the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 3 PULSE TEST. 2. Verify that the results match the expected results. 3. Perform an SVP (Heading 5.1) making sure the histograms display as expected.
C SERVICE AND REPAIR PROCEDURES USER RESOURCE ADAPTER (URA) CARD 4.8 USER RESOURCE ADAPTER (URA) CARD Tools/Supplies Needed B #1 Phillips-head screwdriver B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable. 3. Remove the top cover as directed under Heading 4.3. 4. Locate the URA card (Figure 4.3-1). 5. Remove the screw that fastens the card's metal mounting bracket to the rear of the chassis by using a #1 Phillips-head screwdriver. 6.
SERVICE AND REPAIR PROCEDURES USER RESOURCE ADAPTER (URA) CARD Installation 1. On the new URA card, Figure 4.8-1, verify that: r Jumper X1 is ON r All eight positions of switch SW1 are OFF. 2. Install the URA card into the second, 16-bit expansion slot from the left (facing the instrument). 3. Replace the screw that fastens the card's metal mounting bracket to the rear of the chassis by using a #1 Phillips-head screwdriver (Figure 4.6-1). 4.
C SERVICE AND REPAIR PROCEDURES DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS 4.9 DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS Tools/Supplies Needed B #1 Phillips-head screwdriver B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Remove the top cover as directed under Heading 4.3. 3. Locate the DRA card(s) you are replacing (Figure 4.6-1). 4.
SERVICE AND REPAIR PROCEDURES DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS Installation 1. On the new DRA card(s), verify all the jumper settings (Figure 4.9-1): r X6 - use for both DRA cards. r DRA1 - X5 ON and X4 jumpered for IRQ11. r DRA2 - X5 OFF and X4 jumpered for IRQ12. 2. Install DRA card(s). DRA1 goes into the fourth, 16-bit expansion slot from the left (facing the instrument) and DRA2 goes into the third, 16-bit expansion slot from the left (Figure 4.6-1). 3.
C SERVICE AND REPAIR PROCEDURES MEMBRANE KEYPAD AND DISPLAY 4.10 MEMBRANE KEYPAD AND DISPLAY Tools/Supplies Needed B #2 Phillips-head screwdriver B 1/4 in. nut driver Removal 1. Turn OFF the instrument’s power. 2. Remove the top cover as directed under Heading 4.3. 3. Disconnect the following cables (Figure 4.10-1): 4. a. The display cable (ribbon) from J4 on the URA card. b. The keypad cable (flex) from J5 on the URA card. c.
SERVICE AND REPAIR PROCEDURES MEMBRANE KEYPAD AND DISPLAY 6. Remove the four KEPS nuts and four flat washers (on the back of the display) that fasten the display to the keypad. 7. Remove the display, being careful not to lose any of the four spacers underneath it. Installation 1. Fasten the new display and keypad together: a. Place the spacer hardware on each threaded post. b. Install the display on the posts. c. Place a flat washer on each post. d. Thread the KEPS nut. 2.
C SERVICE AND REPAIR PROCEDURES AC POWER/VACUUM RELAY CARD 4.11 AC POWER/VACUUM RELAY CARD Tools/Supplies Needed B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. WARNING Risk of personal injury. There is a shock hazard if the power cord is not disconnected. Unplug the ac power cable. 2. Disconnect the ac power cable. 3. Open the lower chassis as directed under Heading 4.4 and locate the AC Power/Vacuum Relay card (Figure 4.4-1). 4.
SERVICE AND REPAIR PROCEDURES AC POWER/VACUUM RELAY CARD Verification 1. Turn ON the instrument’s power. 2. Wait for the system to reach the Main Menu. This verifies the operation of relay K2. Note: Power tests are performed during the power-up cycle to verify the +24 V power supply and the Linear Power Supply card voltages. If the tests are passed, the K2 relay is working properly. 4.11-2 3.
C SERVICE AND REPAIR PROCEDURES +24 VOLT SWITCHING POWER SUPPLY 4.12 +24 VOLT SWITCHING POWER SUPPLY Tools/Supplies Needed B #2 Phillips-head screwdriver B Large flat-blade screwdriver Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable. 3. At the rear of the instrument, loosen the three captive thumbscrews (Figure 4.12-1) at the top of the rear panel drop-down door. These thumbscrews are recessed and may require the use of a large flat-blade screwdriver. Figure 4.
SERVICE AND REPAIR PROCEDURES +24 VOLT SWITCHING POWER SUPPLY 6. 7. Disconnect the output and signal wires (Figure 4.12-1). The new part should include a harness with this wiring attached. a. If the harness is included, open up the lower chassis to gain access to the front of the Motor/Solenoid Driver card and disconnect P15. Some wire wraps may also have to be cut to release the harness. b.
SERVICE AND REPAIR PROCEDURES +24 VOLT SWITCHING POWER SUPPLY Verification 1. Turn ON the instrument’s power and allow the system to reach the Main Menu. This verifies the operation of the +24 V power supply. Note: The +24 V turns on when the system software is loaded. An error is displayed on the screen (ERROR DETECTED (015) Power Supply Failure) if there is no +24 V, and the Main Menu is never displayed on the screen.
SERVICE AND REPAIR PROCEDURES +24 VOLT SWITCHING POWER SUPPLY 4.
C SERVICE AND REPAIR PROCEDURES LINEAR POWER SUPPLY CARD 4.13 LINEAR POWER SUPPLY CARD Tools/Supplies Needed B #2 Phillips-head screwdriver B Large flat-blade screwdriver B Voltmeter Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable. 3. At the rear of the instrument, loosen the three captive thumbscrews at the top of the rear panel drop-down door. These thumbscrews are recessed and may require the use of a large flat-blade screwdriver (Figure 4.12-1). 4.
SERVICE AND REPAIR PROCEDURES LINEAR POWER SUPPLY CARD 6. Remove the card from the rear panel, Figure 4.13-2: a. Remove the #2 Phillips-head screw in the center of the card. b. Pull the card off its four snap-type mounting posts. Figure 4.13-2 Back View of MD II with Rear Panel Door Opened T1 LINEAR POWER SUPPLY CARD #2 PHILLIPSHEAD SCREW J1 TEST POINTS JUMPERS (X1, X2, X3) 7242021A Installation 4.13-2 1.
SERVICE AND REPAIR PROCEDURES LINEAR POWER SUPPLY CARD Verification 1. Turn ON the instrument’s power. The Linear Power Supply card should produce voltages immediately. ATTENTION: The Linear Power Supply card is grounded to the Sensor Preamp Adapter card. Since the Sensor Preamp Adapter card is not yet connected, you must use the associated black reference test point with each voltage test point to get a proper reading. 2. Using your voltmeter, verify that you have: r +15 Vdc ±0.75 V r -15 Vdc ±0.
SERVICE AND REPAIR PROCEDURES LINEAR POWER SUPPLY CARD 4.
C SERVICE AND REPAIR PROCEDURES VACUUM SENSOR CARD 4.14 VACUUM SENSOR CARD Tools/Supplies Needed B Needle-nose pliers B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable. 3. Open up the lower chassis as directed under Heading 4.4 and locate the Vacuum Sensor card (Figure 4.4-1). It is preferable to open the chassis door a full 90 degrees. 4. Remove the card by pulling it off the three snap-on posts, Figure 4.
SERVICE AND REPAIR PROCEDURES VACUUM SENSOR CARD 5. Disconnect the three electrical connectors J1, J2, J3. 6. Remove the two tubes coming from the VIC that attach to solenoids LV1 (from FF 9-1) of the normally open port and LV2 (from FF 10-1) of the normally closed port (Figure 4.14-1). Note: Marking these lines before disconnecting them will save having to trace them back on installation. 7. Remove the tubing and adapters connecting pressure transducer port P2 and the common port of LV1 (Figure 4.
C SERVICE AND REPAIR PROCEDURES MOTOR/SOLENOID DRIVER CARD 4.15 MOTOR/SOLENOID DRIVER CARD Tools/Supplies Needed B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable. 3. Open the lower chassis as directed under Heading 4.4 and locate the Motor/Solenoid Driver card (Figure 4.4-1). 4. Remove the Phillips-head shipping screw from the card, if it is still present.
SERVICE AND REPAIR PROCEDURES MOTOR/SOLENOID DRIVER CARD 4. Close the lower chassis. 5. Reconnect the ac power cable. Figure 4.
SERVICE AND REPAIR PROCEDURES MOTOR/SOLENOID DRIVER CARD 3. When the system reaches the Main Menu, perform the Service Diagnostic (Heading 7.3) and check for proper operation of all solenoids and the traverse motors. 4. Verify the operation of the peristaltic pumps: 5. PN 4237242C a. From the Main Menu, select 5 SPECIAL FUNCTIONS tt 3 DILUTER FUNCTIONS. b. Select 4 RINSE to check PM3. c. Select 3 DRAIN to check PM4. d. Select 5 MIX to check PM1. e.
SERVICE AND REPAIR PROCEDURES MOTOR/SOLENOID DRIVER CARD 4.
C SERVICE AND REPAIR PROCEDURES SENSOR PREAMP ADAPTER (SPA) CARD 4.16 SENSOR PREAMP ADAPTER (SPA) CARD Tools/Supplies Needed B #1 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Open the lower chassis as directed under Heading 4.4 and locate the Sensor Preamp Adapter card (Figure 4.4-1). 3. Disconnect the electrical connectors located at the rear of the Sensor Preamp Adapter card. There are five connectors (Figure 4.16-1) and three coaxial cables. Figure 4.
SERVICE AND REPAIR PROCEDURES SENSOR PREAMP ADAPTER (SPA) CARD 4. Remove the cable and card shield covers (Figure 4.16-1): r The cover for the cable shield is at the top of the “tunnel” between the Diluter Panel and the Sensor Preamp Adapter card. It is held in place with two thumbscrews, one on each side. r The cover for the card shield is held in place with two thumbscrews, one in each top corner. The thumbscrews are accessed from the rear of the shield. 5.
SERVICE AND REPAIR PROCEDURES SENSOR PREAMP ADAPTER (SPA) CARD Figure 4.
SERVICE AND REPAIR PROCEDURES SENSOR PREAMP ADAPTER (SPA) CARD 4.
C SERVICE AND REPAIR PROCEDURES PROBE AND PROBE WIPE 4.17 PROBE AND PROBE WIPE Tools/Supplies Needed B #1 Phillips-head screwdriver B Needle-nose pliers B DOW CORNING 33® Lubricant, PN 1604007-0 Removal 1. Using the keypad, proceed as if you were running a sample. When the probe moves to the aspirate position, turn OFF the instrument’s power. 2. Open the lower chassis, as directed under Heading 4.4, to completely expose the Probe/Wipe Traverse Assembly. 3.
SERVICE AND REPAIR PROCEDURES PROBE AND PROBE WIPE 4. If the probe wipe housing is the part to be replaced, disconnect the tubing and then proceed to Installation, step 5. 5. Remove the spring roller near the top of the traverse housing by removing the single Phillips-head retaining screw (Figure 4.17-1). 6. Remove the aspirate probe from the traverse rack (Figure 4.17-1): a.
SERVICE AND REPAIR PROCEDURES PROBE AND PROBE WIPE 5. Install the aspirate tubing onto the probe wipe housing (Figure 4.17-1) if it was removed. The outside large bore tubing of the three-tube ribbon attaches to the top fitting while the middle tubing attaches to the bottom. The top of the probe wipe housing is the end with the groove for the wire retaining clip. 6.
SERVICE AND REPAIR PROCEDURES PROBE AND PROBE WIPE 4.
C SERVICE AND REPAIR PROCEDURES PROBE MOTOR 4.18 PROBE MOTOR Tools/Supplies Needed B #1 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Open the lower chassis as directed in Heading 4.4, to expose the Probe/Wipe Traverse Assembly. 3. Disconnect the probe motor and the flex cable. The probe motor is connected to J2 on the Probe/Opto Sensor card (Figure 4.17-1). 4. Make sure the probe is in a vertical position. Move the traverse housing to the right end of its travel.
SERVICE AND REPAIR PROCEDURES PROBE MOTOR 4.
C SERVICE AND REPAIR PROCEDURES TRAVERSE DRIVE BELT 4.19 TRAVERSE DRIVE BELT Tools/Supplies Needed B #1 Phillips-head screwdriver B Large flat-blade screwdriver Removal 1. Turn OFF the instrument’s power. 2. Open the lower chassis, as directed under Heading 4.4, to completely expose the Probe/Wipe Traverse Assembly. 3. Remove the belt from the idler pulley (Figure 4.19-1): a. Slide the traverse housing to the left to create more slack in the belt near the idler pulley.
SERVICE AND REPAIR PROCEDURES TRAVERSE DRIVE BELT 4. Remove the belt from the tensioner pulley (Figure 4.19-1): a. Slide the traverse housing to the right and loosen the screw that holds the tensioner bracket and pulley in place (traverse belt tensioner bracket screw). Note: You do not have to loosen the traverse belt spring tensioning screw. The tension is set at the factory and should not need to be altered. b.
SERVICE AND REPAIR PROCEDURES TRAVERSE DRIVE BELT CAUTION Risk of component damage. Clamping the belt clamp too far forward or backward produces a bend during normal operation that prematurely wears the belt, traverse housing and traverse guide rod. Tighten the belt clamp on the same plane as the motor pulley to avoid premature wear on the belt, traverse housing and traverse guide rod. 5. Make sure the belt is clamped on the same plane as the motor pulley (Figure 4.19-1).
SERVICE AND REPAIR PROCEDURES TRAVERSE DRIVE BELT 4.
C SERVICE AND REPAIR PROCEDURES TRAVERSE MOTOR 4.20 TRAVERSE MOTOR Tools/Supplies Needed B #2 Phillips-head screwdriver B 1/4 in. nut driver B DOW CORNING 33 Lubricant, PN 1604007-D Removal 1. Turn OFF the instrument’s power. 2. Open the lower chassis, as directed under Heading 4.4, to completely expose the Probe/Wipe Traverse Assembly. 3. Remove the access cover at the top of the Syringe Assembly (Figure 4.20-1). Figure 4.
SERVICE AND REPAIR PROCEDURES TRAVERSE MOTOR 4. Disconnect the traverse motor. The traverse motor connector is to the right (Figure 4.20-1) and is labeled as “J5" and “TRAV MOTOR.” 5. Disconnect the following cables, making sure that these cables are free and can be removed from the instrument: 6. r J15, J16 and J17 (sensor cables from the Solenoid Interconnect card) r J1 flex cable (from the Flex Connect card).
SERVICE AND REPAIR PROCEDURES TRAVERSE MOTOR 6. 7. Connect the electrical connectors: r Flex cable (from Probe/Opto Sensor card) to J1 (on Flex Connect card). r Traverse motor to J5 (behind the Syringe Assembly). r Aspirate position sensor to J17 (on Solenoid Interconnect card). r WBC position sensor to J15 (on Solenoid Interconnect card). r RBC position sensor to J16 (on Solenoid Interconnect card). Replace the access cover, lower the upper chassis and turn ON the instrument’s power.
SERVICE AND REPAIR PROCEDURES TRAVERSE MOTOR 4.
C SERVICE AND REPAIR PROCEDURES SYRINGE ASSEMBLY 4.21 SYRINGE ASSEMBLY Tools/Supplies Needed B #2 Phillips-head screwdriver B 1/4 in. HEX-nut driver B 1/16 in. HEX-key wrench B Large flat-blade screwdriver B LOCTITE® 242 THREADLOCKE2 adhesive, PN 1601018-9 Removal 1. From the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 2 REPLACE SYRINGE. 2. Turn OFF the instrument’s power. 3. Open the lower chassis, as directed under Heading 4.4, to completely expose the Probe/Wipe Traverse Assembly. 4.
SERVICE AND REPAIR PROCEDURES SYRINGE ASSEMBLY Figure 4.21-1 Syringe Assembly SYRINGE MANIFOLDS LUER FITTING (2) HINGE BRACKET HEX-HEAD SCREW (2) SYRINGE GUIDE ROD SCREWS UPPER BEARING UPPER MOUNTING PLATE SPACERS SPRING LEAD SCREW LEAD SCREW ASSEMBLY SYRINGE PLUNGER MOUNTING BRACKET GUIDE ROD GEAR MOTOR SPACER LOWER BEARING SCREW STANDOFF NUT (4) LOOSEN BELT 4.
SERVICE AND REPAIR PROCEDURES SYRINGE ASSEMBLY 12. Free the syringe lead screw assemblies (Figure 4.21-1): a. Remove the two screws fastening the upper mounting plate to the top of the front plate. b. Remove the two screws fastening the syringe guide rods to the upper mounting plate. c. Remove the upper mounting plate with manifolds and sensors still attached. ATTENTION: There are two spacers and one spring for each syringe. Do not lose them, they are critical to syringe operation. 13.
SERVICE AND REPAIR PROCEDURES SYRINGE ASSEMBLY 5. Install the upper mounting plate (Figure 4.21-1): a. Replace the upper lead screw hardware, with a spacer going on first, then the spring, then another spacer. b. Place the upper mounting plate onto the two syringe lead screws and fasten to the two guide rods. Tighten each screw a bit at a time until both are tight. c. Check that the drive belt is still around both gear assemblies. If it is, fasten the upper mounting plate to the face plate. d.
C SERVICE AND REPAIR PROCEDURES PERISTALTIC PUMP MOTOR AND SPOOL 4.22 PERISTALTIC PUMP MOTOR AND SPOOL Tools/Supplies Needed B #2 Phillips-head screwdriver B 1/16 in. HEX-key wrench B DOW CORNING 33 Lubricant, PN 1604007-0 Removal 1. Turn OFF the instrument’s power. 2. Access the Peristaltic Pump Assembly: a. Open the small door on the left side of the unit that holds the diluent reservoir (Figure 4.22-1). Figure 4.
SERVICE AND REPAIR PROCEDURES PERISTALTIC PUMP MOTOR AND SPOOL 3. Remove the Peristaltic Pump Assembly from the lower chassis. It is held in place with six Phillips-head screws (Figure 4.22-1). There should be enough play in the tubing and wiring to allow work on the assembly without disconnecting it. 4. Remove the peristaltic tubing from the pump (Figure 4.22-1). 5. Remove the tubing spool from the motor shaft (Figure 4.22-1).
C SERVICE AND REPAIR PROCEDURES VACUUM PUMP 4.23 VACUUM PUMP Tools/Supplies Needed B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Disconnect the ac power cable. 3. Open the lower chassis, as directed under Heading 4.4, and locate the vacuum pump (Figure 4.4-1). 4. Disconnect the electrical connector for the vacuum pump. 5. Disconnect the single tubing connected to the left end of the pump. 6. Remove the vacuum pump from the instrument.
SERVICE AND REPAIR PROCEDURES VACUUM PUMP 4.
C SERVICE AND REPAIR PROCEDURES APERTURE, BATH AND APERTURE ELECTRODE MODULE 4.24 APERTURE, BATH AND APERTURE ELECTRODE MODULE Tools/Supplies Needed B #2 Phillips-head screwdriver Removal 1. Drain the baths. 2. Turn OFF the instrument’s power. 3. Open the lower chassis as directed in Heading 4.4. 4. Gain access to the aperture area by removing the Diluter Panel shield with door. The shield is held in place with three captive thumbscrews. 5.
SERVICE AND REPAIR PROCEDURES APERTURE, BATH AND APERTURE ELECTRODE MODULE 7. Remove the thumbscrew at the front of the aperture bath assembly (Figure 4.24-1). The bath, aperture, and rear module are pressure-fit and this thumbscrew provides the pressure. 8. Remove the aperture bath. To get enough room at the front of the bath to clear the aperture block, you may have to remove the metal insert (Figure 4.24-1) that the thumbscrew presses against. 9.
SERVICE AND REPAIR PROCEDURES APERTURE, BATH AND APERTURE ELECTRODE MODULE 2. 3. 4. Install the aperture block (Figure 4.24-1): a. Verify that there are small O-rings at the top and bottom of the block. b. Verify that the O-rings are in good condition. If they are too flat or beginning to deteriorate, replace them. c. Verify that the area around the aperture bath is dry. d. Tighten the fitting below the aperture block.
SERVICE AND REPAIR PROCEDURES APERTURE, BATH AND APERTURE ELECTRODE MODULE 4.
C SERVICE AND REPAIR PROCEDURES DILUTER PANEL SOLENOIDS 4.25 DILUTER PANEL SOLENOIDS Tools/Supplies Needed B #2 Phillips-head screwdriver Removal 1. Turn OFF the instrument’s power. 2. Open the lower chassis as directed in Heading 4.4. 3. Locate the Solenoid Interconnect card (Figure 4.4-1). 4. Disconnect the connector for the solenoid you are replacing and free the cable of any wire ties or obstructions so that the connector can easily be pulled through the Diluter Panel from the front. 5.
SERVICE AND REPAIR PROCEDURES DILUTER PANEL SOLENOIDS 4.
C SERVICE AND REPAIR PROCEDURES SWEEP-FLOW TUBING 4.26 SWEEP-FLOW TUBING Tools/Supplies Needed B #2 Phillips-head screwdriver B Needle-nose pliers B Small-side cutter Removal 1. Open the lower chassis as directed in Heading 4.4. 2. Gain access to the aperture area by removing the Diluter Panel shield with door. The shield is held in place with three captive thumbscrews. 3. Disconnect the sweep-flow tubing.
SERVICE AND REPAIR PROCEDURES SWEEP-FLOW TUBING Installation 1. Wind 13 ft (evenly back and forth) of PN 3202220-1 tubing onto the spool, being careful to leave 4 in. at one end and 5 in. at the other end. The tubing must all fit in the spool when wound. 2. Thread the exposed ends of the tubing through the face plate and place the spool in its cavity (Figure 4.26-1). 3. Fasten the face plate to the Diluter Panel (Figure 4.26-1). 4. Connect the 4 in.
C SERVICE AND REPAIR PROCEDURES LATEX GAIN ADJUSTMENT 4.27 LATEX GAIN ADJUSTMENT Tools/Supplies Needed B Latex aperture gain particles, PN 6857371-8 Procedure 1. Ensure that the instrument is primed and ready to run a sample. 2. At the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 1 LATEX GAIN. 3. Enter the latex particle size from the assay value stated on the container. 4. Make sure the latex aperture gain particles are well mixed, then aspirate them as directed on the display. 5.
SERVICE AND REPAIR PROCEDURES LATEX GAIN ADJUSTMENT 4.
C SERVICE AND REPAIR PROCEDURES AIM ADJUSTMENT 4.28 AIM ADJUSTMENT Tools/Supplies Needed B Five different whole-blood specimens with normal parameters (especially for WBC) Note: Since nomal parameters do not guarantee a normal AIM result, it is preferable to use different blood. Procedure 1. Bleach the apertures. Refer to the How to Clean the Baths procedure in the Operator’s Guide. 2. Run each of the five specimens once. After each sample, access the Service Report (see Heading 7.
SERVICE AND REPAIR PROCEDURES AIM ADJUSTMENT Option 2 Many customers in the MD II market do not have samples of blood at installation, or have only fingerpick samples, which are not ideal for AIM adjustment. Though not preferred, 4C PLUS cell control can be used to adjust the AIM values. This method produces an RBC 26th percentile target similar to the preferred fresh whole-blood results. For WBC, the value is about 1.5% lower than the value produced using fresh whole blood.
C SERVICE AND REPAIR PROCEDURES HGB PREAMP ADJUSTMENT 4.29 HGB PREAMP ADJUSTMENT Tools/Supplies Needed B #2 Phillips-head screwdriver B DVM with a 3 1/2 digit display B Pot adjustment tool or small-blade screwdriver Procedure 1. Ensure the system is primed and that there is clear diluent in the WBC bath. 2. Open the lower chassis, as directed under Heading 4.4, and locate the Hgb Preamp card (Figure 4.4-1). 3. Adjust the Hgb-blank reading.
SERVICE AND REPAIR PROCEDURES HGB PREAMP ADJUSTMENT 4.
C SERVICE AND REPAIR PROCEDURES VACUUM ADJUSTMENT 4.30 VACUUM ADJUSTMENT Procedure PN 4237242C 1. At the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 5 VACUUM ADJUST. 2. Using the vacuum regulator (Figure 4.20-1) in the upper-right corner of the fluidics panel, adjust as close to 6.00 in. Hg as possible. The system tolerance limit for vacuum is 5.83 to 6.17 in. Hg. 4.
SERVICE AND REPAIR PROCEDURES VACUUM ADJUSTMENT 4.
C SERVICE AND REPAIR PROCEDURES LYSE VOLUME ADJUSTMENT/VERIFICATION 4.31 LYSE VOLUME ADJUSTMENT/VERIFICATION Note: This procedure need not be performed frequently. The solenoid pump is very accurate and stable. Once adjusted correctly, it should not require readjustment. Any drift or variability in the volume is most likely caused by a problem elsewhere in the lyse system. Tools/Supplies Needed B Lyse volume-adjustment measuring vial, PN 5415483-6, or a 5-mL graduated measuring device B 9/16 in.
SERVICE AND REPAIR PROCEDURES LYSE VOLUME ADJUSTMENT/VERIFICATION 4.
C CONTENTS 5 MAINTENANCE PROCEDURES, 5.1-1 5.1 5.2 PN 4237242C SYSTEM VERIFICATION PROCEDURE (SVP), 5.1-1 PMI RECOMMENDATIONS, 5.2-1 Components, 5.2-1 Filters, 5.2-1 Peristaltic Pump Tubing, 5.2-1 Polyurethane Tubing, 5.2-1 Syringes, 5.2-1 Cleaning, 5.2-1 Adjustment Procedures, 5.2-2 Verification Procedures, 5.
CONTENTS 5-ii PN 4237242C
C 5MAINTENANCE PROCEDURES 5 5.1 SYSTEM VERIFICATION PROCEDURE (SVP) PN 4237242C 1. Inspect the blue and green filters and change if required. Peristaltic pump tubing wear requires that the blue filters connected to the diluent and rinse pumps should be changed every 9,000 cycles. 2. Inspect the peristaltic pump tubing. If it shows excessive wear, or it has been in service for 18,000 cycles, the tubing should be replaced.
MAINTENANCE PROCEDURES SYSTEM VERIFICATION PROCEDURE (SVP) 5.
C MAINTENANCE PROCEDURES PMI RECOMMENDATIONS 5.2 PMI RECOMMENDATIONS The MD II is a low maintenance instrument that does not require a PMI. Those service organizations that will perform PMIs on this instrument can consider the following components and verifications for their procedure. Components Filters The green fluid barriers and blue particle filters should be replaced on every PMI.
MAINTENANCE PROCEDURES PMI RECOMMENDATIONS Adjustment Procedures 1. Perform the Latex Gain Adjustment procedure (Heading 4.27). 2. Perform the AIM Adjustment procedure (Heading 4.28). 3. Perform the Hgb Preamp Adjustment procedure (Heading 4.29). Verification Procedures 5.2-2 1. Perform the REPRODUCIBILITY AND CARRYOVER function found in the Calibration section of the Operator’s Guide. Both these tests must PASS. 2. Have the customer run their controls.
C CONTENTS 6 SCHEMATICS AND BLOCK DIAGRAMS, 6.1-1 6.1 DOCUMENT CONTROL NUMBERS AND DESCRIPTIONS, 6.1-1 6.2 SCHEMATICS, 6.2-1 TABLES 6.1-1 6.1-2 PN 4237242C DCNs and Descriptions for Schematics Included in This Manual, 6.1-1 DCNs and Descriptions for Schematics Not Included in This Manual, 6.
CONTENTS 6-ii PN 4237242C
C 6SCHEMATICS AND BLOCK DIAGRAMS 6 6.1 DOCUMENT CONTROL NUMBERS AND DESCRIPTIONS IMPORTANT Risk of instrument malfunction or erroneous results. Official schematics and drawings in this section will be revised only with revision of the manual. They will be current on the date of the first printing of a revision. Reprints between manual revisions will not be updated with current drawing revisions. It is the user's responsibililty to update these drawings between manual revisions Table 6.
SCHEMATICS AND BLOCK DIAGRAMS DOCUMENT CONTROL NUMBERS AND DESCRIPTIONS 6.
C SCHEMATICS AND BLOCK DIAGRAMS SCHEMATICS 6.2 SCHEMATICS This section includes the schematics and/or diagrams for the: PN 4237242C r Pneumatic/Hydraulic Layout r Timing Charts r Electrical Interconnect Diagram r Linear Power Supply Card r AC Power/Vacuum Relay Card r Motor/Solenoid Driver Card. 6.
SCHEMATICS AND BLOCK DIAGRAMS SCHEMATICS 6.
C CONTENTS 7 TROUBLESHOOTING, 7.1-1 7.1 TROUBLESHOOTING DIAGNOSTICS, 7.1-1 Customer Options Available for Troubleshooting, 7.1-1 Service Menu, 7.1-2 7.2 GENERATING A SERVICE REPORT, 7.2-1 The Screen Display, 7.2-1 The Printed Report, 7.2-1 AIM Criteria Results, 7.2-2 Hgb Data, 7.2-3 Sample Results - DO NOT REPORT, 7.2-3 7.3 SERVICE DIAGNOSTIC, 7.3-1 7.4 TROUBLESHOOTING ERROR MESSAGES, 7.4-1 7.5 TROUBLESHOOTING POWER-UP PROBLEMS, 7.5-1 ILLUSTRATIONS 7.2-1 7.2-2 7.2-3 Sample Screen Display, 7.
CONTENTS 7-ii PN 4237242C
C 7TROUBLESHOOTING 7 7.1 TROUBLESHOOTING DIAGNOSTICS Customer Options Available for Troubleshooting The Diluter Functions menu provides the customer and Service Representative a means of basic diluter troubleshooting. There are six functions provided on this menu and they are described in Table 7.1-1. Table 7.1-1 Diluter Functions Menu Options Function Description CLEAR APERTURES Performs an aperture burn or zap, similar to the zap performed during an aspiration cycle.
TROUBLESHOOTING TROUBLESHOOTING DIAGNOSTICS Service Menu The Service menu contains six visible menu items. These are items the customer has access to but will not often use. They are of most use during troubleshooting and are discussed in Table 7.1-2. There is a seventh item, Service Diagnostic, that is hidden from the customer. Table 7.1-2 Service Menu Options Function Description When to Use LATEX GAIN (for Coulter service personnel only) Adjusts the RBC and WBC aperture current settings.
C TROUBLESHOOTING GENERATING A SERVICE REPORT 7.2 GENERATING A SERVICE REPORT The Service Report provides data about the previous aspirate cycle, whether it was a sample, a control or a calibrator. This data is not displayed on any customer screen or printout. To access the Service Report, at the Main Menu select 5 SPECIAL FUNCTIONS tt 4 SUPERVISOR tt 7 [no menu item displays on the screen].
TROUBLESHOOTING GENERATING A SERVICE REPORT Figure 7.2-2 Sample Printed Report 7242044A AIM Criteria Results During data accumulation, pulses are edited so that only good pulses are used to produce histograms. One output of the Editor circuit is the 26-percentile voltage. This voltage is a pulse width parameter that is representative of the travel time or flow through the aperture.
TROUBLESHOOTING GENERATING A SERVICE REPORT Ratio CV: The ratio of good pulses to total pulses is calculated for each of the 12 count periods, then the CV of the 12 ratios is calculated. Voteout: The 12 count periods are grouped into 3 sets of 4 count periods, each set being a logical aperture. If one "aperture" count is too far removed from the other two, it is "voted out" and its number is given. If there is no voteout or a total voteout, a zero is given.
TROUBLESHOOTING GENERATING A SERVICE REPORT Figure 7.2-3 Hgb Results Analysis IS BLANK #1 OR BLANK #2 < 2.5 VOLTS ? YES NON-FATAL ERROR: Hgb BLANK VOLTAGE LOW NO IS BLANK 1 OR BLANK 2 > 4.95 VOLTS ? YES NON-FATAL ERROR: Hgb BLANK VOLTAGE HIGH NO CALCULATE A Hgb USING BOTH BLANK READINGS IS RESULT > 0.6 ? YES GENERATE INCOMPLETE Hgb RESULT NO USING HIGHEST BLANK READING FOR BOTH, CALCULATE THE Hgb 1 AND Hgb 2 RESULT IS Hgb RESULT DIFFERENCE > 0.
C TROUBLESHOOTING SERVICE DIAGNOSTIC 7.3 SERVICE DIAGNOSTIC A Service Diagnostic diluter table (or cycle) has been included in the instrument. The table energizes solenoids and motors, one by one. ATTENTION: Once you begin the cycle, you must advance completely through the table. Pressing the Escape key will not exit the cycle. To begin the Service Diagnostic, at the Main Menu select 5 SPECIAL FUNCTION tt 5 SERVICE tt 7 [no menu item displays on the screen].
TROUBLESHOOTING SERVICE DIAGNOSTIC 7.
C TROUBLESHOOTING TROUBLESHOOTING ERROR MESSAGES 7.4 TROUBLESHOOTING ERROR MESSAGES Table 7.4-1 Error Messages Error Message Code Description/Possible Fault Aspirate Syringe Failure 026 When the syringe is sent to the home position, it stops on sensor. To make sure it gets to the sensor, it is allowed 40 extra steps. This means that if the motor is sent down a total of 3000 steps, it is sent up 3040 steps.
C TROUBLESHOOTING TROUBLESHOOTING ERROR MESSAGES Table 7.4-1 Error Messages (Continued) Error Message Code Description/Possible Fault DRA Board Failure 003 When a stepper motor is set in operation, the DRA card uses a hardware timer to control the task. When the task is completed, the DRA card generates an interrupt request signaling that the task is complete. An independent software timer is allocated whenever a motor is given a task.
TROUBLESHOOTING TROUBLESHOOTING ERROR MESSAGES Table 7.4-1 Error Messages (Continued) Error Message Code Description/Possible Fault Power Supply Failure 015 The +24 V function checks the state of two latches. One latch is tied to the POWERFAIL signal from the +24 V power supply, and the other is controlled by the Overload Timer circuit on the Motor/Solenoid Driver card.
TROUBLESHOOTING TROUBLESHOOTING ERROR MESSAGES Table 7.4-1 Error Messages (Continued) Error Message Code Description/Possible Fault RAM Drive Failure 038 During system boot, Virtual RAM (VRAM) drives are created. If the system was unable to create a drive, ERROR DETECTED (038) RAM Drive Failure is displayed on the screen. To create a VRAM drive, you must put the proper line in the CONFIG.SYS file. This means that the drive is created very early in the boot sequence.
TROUBLESHOOTING TROUBLESHOOTING ERROR MESSAGES Table 7.4-1 Error Messages (Continued) Error Message Code Description/Possible Fault Unable to Create INF File Non-fatal Unable to Create INF File is displayed on the screen when the system attempts Error to create an INF file and cannot. This message does not stop the system or the report for the sample being analyzed. It is merely a message for the user's information. Creation of the INF file occurs only if SW1-2 of the URA card is ON.
TROUBLESHOOTING TROUBLESHOOTING ERROR MESSAGES 7.
C TROUBLESHOOTING TROUBLESHOOTING POWER-UP PROBLEMS 7.5 TROUBLESHOOTING POWER-UP PROBLEMS The flowchart in Figure 7.5-1can help you troubleshoot when the instrument does not reach the Main Menu on power up. Figure 7.5-1 Power-Up Troubleshooting Flowchart (part 1 of 2) TURN POWER ON TURNS ON LINEAR PS & SUPPLIES AC FOR +24 V +12 V TO K1 ON AC POWER/ VACUUM RELAY CARD POST BEEP CODES: APPENDIX TABLE C.
TROUBLESHOOTING TROUBLESHOOTING POWER-UP PROBLEMS Figure 7.
C CONTENTS 8 PARTS LISTS, 8.1-1 8.1 MASTER PARTS LISTS, 8.1-1 8.2 ILLUSTRATED PARTS, 8.2-1 ILLUSTRATIONS 8.2-1 8.2-2 8.2-3 8.2-4 8.2-5 8.2-6 8.2-7 8.2-8 8.2-9 MD II, Lower Chassis, Left Side View (See Table 8.2-1), 8.2-1 MD II, Front View (See Table 8.2-2), 8.2-2 MD II, Top View into Lower Chassis (See Table 8.2-3), 8.2-3 MD II, Upper Chassis (See Table 8.2-4), 8.2-4 MD II, Back View (See Table 8.2-5), 8.2-5 Traverse Assembly (See Table 8.2-6), 8.2-6 Diluter Assembly (See Table 8.2-6), 8.
CONTENTS 8-ii PN 4237242C
C 8PARTS LISTS 8 8.1 MASTER PARTS LISTS These parts lists are in part number order by category. The categories are: r Cables r Lower Chassis r Peripherals and Support r Upper Chassis r Miscellaneous Hardware. Table 8.1-1 Cables Part No. Description Figure Item 6027225-5 Cable, main ac power cable to instrument 8.2-5 10 6027767-2 Cable, power line cord, ac diluter to analyzer 8.2-5 2 6028504-7 Cable, Centronics parallel Printer, 10 ft 8.
PARTS LISTS MASTER PARTS LISTS Table 8.1-2 Lower Chassis Part No. Description Figure Item 1017541-1 Reservoir, diluent (uses cover, PN 1019609-4) 8.2-1 1 1019609-4 Cover, for diluent reservoir (PN 1017541-1) 8.2-1 2 1022803-4 Housing, 2-port manifold for diluent syringe, molded 1020601-4 Housing, 3-port manifold for aspirate syringe, molded 8.2-8 10 1021228-6 Fitting, top of aspirate and diluent syringe block 8.
PARTS LISTS MASTER PARTS LISTS Table 8.1-2 Lower Chassis (Continued) Part No. Description Figure Item 4004092-7 Power supply, +24 V switching 8.2-3 1 8.2-5 6 4717896-7 Resistor, 25 W, wire-wound, 15 Ω for motor drivers 8.2-5 7 5102018-9 Fuse, 4 A, 250 V, (F1 and F2 at back, 120 V instrument) 8.2-5 9 5102021-9 Fuse, 2 A, 250 V, (F1 and F2 at back, 220 V instrument) 8.2-5 9 5102084-7 Fuse, 0.
PARTS LISTS MASTER PARTS LISTS Table 8.1-2 Lower Chassis (Continued) Part No. Description Figure Item 6706161-6 Card, Probe/Opto Sensor 8.2-6 8 6706166-7 Card, Flex Connect 8.2-3 10 6706202-7 Card, Solenoid Interconnect 8.2-3 9 6706254-0 Sensor, lyse fluid detector assembly not shown 6706321-0 Guide, traverse housing, lower, machined not shown 6706322-8 Rack, traverse housing with pin not shown 6706323-6 Card, Hgb Preamp 8.2-3 7 6805010-3 Sensor, Hgb photodetector 8.
PARTS LISTS MASTER PARTS LISTS Table 8.1-3 Peripherals and Support Part No.
PARTS LISTS MASTER PARTS LISTS Table 8.1-3 Peripherals and Support (Continued) Part No. Description Figure Item 6856742-4 Pickup tube, waste not shown 6857371-8 Particles, latex aperture gain not shown 6913269-3 Kit, Host Communication, with adapter and manual not shown 6914956-1 Tool, lyse volume-adjustment measuring vial kit, MD Series not shown 6915032-2 Software, service replacement Ticket Key Disk Kit not shown Table 8.1-4 Upper Chassis Part No.
PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Miscellaneous Hardware Part No. Description Figure Item 1022792-5 Washer, rubber, used to mount old style Hgb LED & photodiode 8.2-7 14 1022916-2 Bushing, front aperture bath clamp 8.
PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Miscellaneous Hardware (Continued) Part No. Description 2827147-6 Washer, flat, #6, used to fasten syringe plunger 2827148-4 Washer, flat, #8 8.2-8 17 2851795-5 Screw, captive 6-32 thumbscrew 8.2-2 6 2851835-8 Spacer, #10 St. St. (stainless steel) 8.2-8 21 2851837-4 Spacer, lead screw 8.2-8 12 2851848-0 Nut, 10-32, square 8.2-9 11 2851905-2 Screw, aperture bath mounting thumbscrew 8.
C PARTS LISTS ILLUSTRATED PARTS 8.2 ILLUSTRATED PARTS Note: na = part number is not available Figure 8.2-1 MD II, Lower Chassis, Left Side View (See Table 8.2-1) 3 2 1 4 5 6 8 14 13 12 11 10 9 7 Table 8.2-1 MD II, Lower Chassis, Left Side View (See Figure 8.
PARTS LISTS ILLUSTRATED PARTS Figure 8.2-2 MD II, Front View (See Table 8.2-2) 2 1 7 4 1 _ 8 5 2 0 9 6 3 . 3 11 000000 4 10 5 9 8 7 6 7242027B Table 8.2-2 MD II, Front View (See Figure 8.2-2) Item Part Number Description 1 7000168-3 Display, LCD main 2 2016601-1 Keypad, membrane touch pad (use while available) 2016722-0 Keypad, membrane touch pad, tactile response 3 6232628-0 Regulator, vacuum 4 2523618-1 Syringe, glass, 100 µL 1%, aspirate 5 2523638-6 Syringe, glass, 5.
C PARTS LISTS ILLUSTRATED PARTS Figure 8.2-3 MD II, Top View into Lower Chassis (See Table 8.2-3) 1 2 3 -V4 + -V3 + -2V + +S -S ON/OFF PF/PG A C P O W ER /V AC U U M V1 + RTN RTN + 4 11 MOTOR/SOLENOID DRIVER CARD 5 10 6 9 8 7 7242028A Table 8.2-3 MD II, Top View into Lower Chassis (See Figure 8.
C PARTS LISTS ILLUSTRATED PARTS Figure 8.2-4 MD II, Upper Chassis (See Table 8.2-4) 2 3 5 4 J15 SER1 6 P8 J17 FLOPPY J18 PARALLEL J14 SER2 1 P9 SW1 J20 IDE J19 EXT BATT BATTERY 7 BANK 0 BANK 1 BANK 2 BANK 3 W3 10 9 8 7242029A Table 8.2-4 MD II, Upper Chassis (See Figure 8.
PARTS LISTS ILLUSTRATED PARTS Figure 8.2-5 MD II, Back View (See Table 8.2-5) 2 1 3 1 MD II TICKET PRINTER (OPTIONAL) PRINTER COMPUTER POWER 4 AC POWER 11 10 9 8 -V3 + -2V + +S -S ON/OFF PF/PG -V4 + V1 + RTN RTN 7 + 6 5 7242030B Table 8.2-5 MD II, Back View (See Figure 8.
C PARTS LISTS ILLUSTRATED PARTS Figure 8.2-6 Traverse Assembly (See Table 8.2-6) 7 8 6 5 1 9 4 37 38 1 39 3 2 1 36 35 10 34 11 33 32 12 13 19 20 18 14 21 6 15 31 22 30 29 16 17 23 28 27 24 26 25 REAR OF TRAVERSE HOUSING 8.
C PARTS LISTS ILLUSTRATED PARTS Table 8.2-6 Traverse Assembly (See Figure 8.
PARTS LISTS ILLUSTRATED PARTS Figure 8.2-7 Diluter Assembly (See Table 8.2-7) 1 16 17 2 3 19 18 15 14 5 4 13 12 11 10 6 9 8.
PARTS LISTS ILLUSTRATED PARTS Table 8.2-7 Diluter Assembly (See Figure 8.
C PARTS LISTS ILLUSTRATED PARTS Figure 8.2-8 Syringe Assembly (See Table 8.2-8) 11 10 9 12 8 7 5 6 5 13 1 14 13 4 15 2 16 3 17 23 22 21 18 19 20 7242033A 8.
PARTS LISTS ILLUSTRATED PARTS Table 8.2-8 Syringe Assembly (See Figure 8.2-8) Item Part Number Description 1 6805068-5 Motor, stepper assembly 2 2523628-9 Gear, used on lead screw, syringe and traverse motor 3 2523630-1 Belt, syringe lead screw drive 4 2523625-4 Bearing, syringe lead screw (used top and bottom) 5 6805024-3 Sensor, optical assembly used on syringe module 6 2852093-0 Screw, 6-32 x 0.38 in.
PARTS LISTS ILLUSTRATED PARTS Figure 8.2-9 Peristaltic Pump Assembly (See Table 8.2-9) 1 13 2 12 3 4 11 10 9 8 5 6 7 7242034A 8.
PARTS LISTS ILLUSTRATED PARTS Table 8.2-9 Peristaltic Pump Assembly (See Figure 8.2-9) Item Part Number Description 1 1014052-8 Washer, stepper motor wear plate 2 6805068-5 Motor, stepper assembly 3 6859650-5 Spool, peristaltic pump tubing 4 2827024-1 Screw, 6-32 HEX head setscrew, peristaltic spool 5 6232246-2 Fitting, plastic reducer, peristaltic pump tubing 6 3213214-6 Tubing, silicon, peristaltic pump 7 2852093-0 Screw, machine, 6-32 x 0.38 in.
PARTS LISTS ILLUSTRATED PARTS 8.
C CONTENTS A B QUICK REFERENCE INFORMATION, A.1-1 A.1 TOLERANCES, VOLTAGES AND LIMITS, A.1-1 Limits, A.1-1 Adjustments, A.1-1 A.2 SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS, A.2-1 AC Power/Vacuum Relay Card, A.2-1 Connectors, A.2-1 Test Points, A.2-1 AT Motherboard, A.2-1 DRA Card, A.2-2 Hgb Preamp Card, A.2-2 Test Points, A.2-2 Linear Power Supply Card, A.2-2 Jumpers, A.2-2 Test Points, A.2-2 Motor/Solenoid Driver Card, A.2-3 Jumpers, A.2-3 Test Points, A.2-3 Sensor Preamp Adapter Card, A.
CONTENTS E INTELLIGENT SOFTWARE LINK (ISL) OPTION, E.1-1 E.1 ISL OPTION FOR RALS, E.1-1 ISL Description, E.1-1 RALS Installation, Training and Service Responsibilities, E.1-1 Recognizing an MD II Linked to RALS, E.1-1 Servicing an MD II Linked to RALS, E.1-1 ILLUSTRATIONS A.3-1 CITIZEN GSX-190 Printer Settings, A.3-1 A.5-1 Software Menu Tree, A.5-1 D.1-1 Epson TM-290P Slip Printer Control Locations, D.1-1 TABLES A.1-1 Calibration Factor Limits, A.1-1 A.1-2 Amplifier Noise Limits, A.1-1 A.
C AQUICK REFERENCE INFORMATION A A.1 TOLERANCES, VOLTAGES AND LIMITS Limits Table A.1-1 Calibration Factor Limits Parameter Expected Range WBC 0.975 to 1.169 RBC 1.079 to 1.251 Hgb 0.774 to 0.898 MCV 0.897 to 0.949 Plt 1.004 to 1.214 MPV 1.074 to 1.278 ATTENTION: Millivolts (mV) must be measured with a true rms (root mean square) DMM (digital multi-meter). The FLUKE® Model 8920A or its equivalent is acceptable. Table A.
C QUICK REFERENCE INFORMATION TOLERANCES, VOLTAGES AND LIMITS A.
C QUICK REFERENCE INFORMATION SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS A.2 SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS AC Power/Vacuum Relay Card Connectors Table A.2-1 AC Power/Vacuum Relay Card Connectors and Line Input Ranges Connector Range 100 VOLTS 90 - 110 Vac 120 VOLTS 110 - 132 Vac 220 VOLTS 198 - 242 Vac 240 VOLTS 220 - 264 Vac Test Points TP1, TP2-ac in AT Motherboard Table A.
QUICK REFERENCE INFORMATION SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS DRA Card Table A.2-3 DRA Card Jumper Settings Jumper Description MD II Setting X4 Sets card for IRQ11, needed for DRA1 1-3 Sets card for IRQ12, needed for DRA2 2-3 Sets card to DRA1 or DRA2 ON - DRA1 X5 OFF - DRA2 X6 Connects oscillator to circuit when jumped ON Hgb Preamp Card Test Points TP1 - orange, output TP2 - black, ground Linear Power Supply Card Jumpers Table A.
QUICK REFERENCE INFORMATION SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS Motor/Solenoid Driver Card Jumpers Table A.2-6 Motor/Solenoid Driver Card Jumper Settings Jumper Description MD II Setting X1 +24 V control ON X2 ON - Connects oscillator to circuit ON OFF - Disconnects oscillator for card testing Test Points Table A.
QUICK REFERENCE INFORMATION SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS Sensor Preamp Adapter Card Table A.2-8 Sensor Preamp Adapter Card Jumper Settings Jumper Description MD II Setting X3 Grounds WBC Dc Restorer input for subassembly adjustment and testing. OFF X4 Connects WBC Preamp output to Dc Restorer. ON X7 Connects RBC Preamp output to Dc Restorer. ON X8 Grounds RBC Dc Restorer input for subassembly adjustment and testing.
C QUICK REFERENCE INFORMATION PRINTER SETTINGS A.3 PRINTER SETTINGS Figure A.3-1 shows the MD II settings for the CITIZEN GSX-190 Printer. If that is the Printer at this site, use these settings. Figure A.3-1 CITIZEN GSX-190 Printer Settings PN 4237242C A.
QUICK REFERENCE INFORMATION PRINTER SETTINGS A.
C QUICK REFERENCE INFORMATION FUNCTIONS A.4 FUNCTIONS Peristaltic Pumps Table A.4-1 Peristaltic Pumps Location and Function PN 4237242C Pump Location Function PM1 (Air/Mix) Upper right Provides air for isolation and mixing bubbles. PM2 (Diluent) Upper left Fills the diluent reservoir from the diluent tube. PM3 (Rinse) Lower left Rinses the baths with fluid from the diluent reservoir. PM4 (Waste) Lower right Drains the VIC and both red and white baths. A.
QUICK REFERENCE INFORMATION FUNCTIONS A.
C QUICK REFERENCE INFORMATION MENU TREE A.5 MENU TREE Figure A.5-1 Software Menu Tree 1. RUN SAMPLE ENTER ID ENTER RANGE PASSWORD CONTROL LEVEL OPTIONS 1. LOW 2. NORMAL 3. HIGH 2. RUN CONTROL 3. STARTUP ASPIRATE RUN CONTROL 4. REVIEW LAST SAMPLE 1. SETUP 1. DATE/TIME 2. IQAP ID # 1. SELECT DATE FORMAT 2. CHANGE DATE AND TIME 3. HOST SETTINGS 1. HOST COMMUNICATION 2. BAUD RATE 3. DATA BITS 4. PARITY 5. STOP BITS 4. OPTIONS 1. PRINT 1. AUTOMATIC 2. MANUAL 2. CONTROL 1.
C QUICK REFERENCE INFORMATION MENU TREE A.
C BPROCEDURES FOR SPECIAL TOOLS OR SOFTWARE B B.1 PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE A Service Diagnostic disk will eventually be available for this product. When it becomes available, this section will contain instructions on its use. PN 4237242C B.
C PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE B.
C CMESSAGE/ERROR CODE LISTINGS C C.1 INSTRUMENT NON-FATAL ERROR MESSAGES Table C.1-1 Non-fatal Error Messages Displayed Message Description Hgb Blank Voltage High An Hgb-blank reading was greater than 4.95 V. Hgb Blank Voltage Low An Hgb-blank reading was less than 2.50 V. Unable to Create INF File The system attempted to create an INF file but was unable to do so. Vacuum out of Range The vacuum for one of the 12, one-second count periods was outside 5.83 to 6.17 in. Hg. PN 4237242C C.
MESSAGE/ERROR CODE LISTINGS INSTRUMENT NON-FATAL ERROR MESSAGES C.
C C MESSAGE/ERROR CODE LISTINGS INSTRUMENT FATAL ERROR MESSAGES C.2 INSTRUMENT FATAL ERROR MESSAGES Table C.2-1 Fatal Error Messages Error Code Displayed Message Description 001 SPAD Board Failure Currently not used. 002 URA Board Failure Currently not used. 003 DRA Board Failure A motor driver did not generate an interrupt to indicate that it finished an operation. 004 SPAD Board Failure While channelizing pulses, a buffer overload occurred.
MESSAGE/ERROR CODE LISTINGS INSTRUMENT FATAL ERROR MESSAGES Table C.2-1 Fatal Error Messages (Continued) Error Code Displayed Message Description 031 Probe Mechanism Failure The probe was at the top position when it should not have been. 032 Probe Mechanism Failure The probe was not above the WBC bath when it should have been. 033 Probe Mechanism Failure The probe was not above the RBC bath when it should have been.
C C MESSAGE/ERROR CODE LISTINGS AMIBIOS BEEP CODES C.3 AMIBIOS BEEP CODES Beep codes (Table C.3-1) are errors found during the first stage of the Power On Self Test (POST). All beep code errors except beep code 8 are fatal errors. A fatal error does not allow the boot process to continue. Errors occurring after this phase are non fatal and are displayed to a computer monitor. The MD II’s display is not a computer monitor and is driven by the system software and hardware.
MESSAGE/ERROR CODE LISTINGS AMIBIOS BEEP CODES C.
C DOPTIONAL PRINTERS D D.1 EPSON TM-290P SLIP PRINTER Specifications Print method: Impact dot matrix Shuttle-type, 7 pin head Unidirectional printing We use a 0.63 mm column spacing We use 4.23 mm (1/6 in.) line spacing Ribbon: Exclusive ribbon cassette Type ERC-27 Purple ink Life expectancy about 1,500,000 characters Paper: Types - Normal (High Quality), pressure sensitive, carbon copy Total thickness for single-ply paper (no copy) - 0.09 to 0.
OPTIONAL PRINTERS EPSON TM-290P SLIP PRINTER DIP Switch SW1 Settings Table D.1-1 DIP Switch SW1 Position Settings Positions/Settings 1 - OFF 2 - ON 3 - ON 4 - ON ON = Autofeed International Character Sets - See Table D.1-2 5 - OFF 6 - OFF 7 - OFF 8 - OFF 9 - OFF 10 - OFF Not Used Not Used Not Used Not Used Not Used Not Used Table D.1-2 DIP Switch SW1 Settings for International Character Sets Position Settings Country SW1- 2 SW1- 3 SW1- 4 U.S.A.
C EINTELLIGENT SOFTWARE LINK (ISL) OPTION E E.1 ISL OPTION FOR RALS ISL Description The intelligent software link (ISL) is a software protocol designed to allow a host system to operate the user interface portion of a Coulter instrument remotely. Since this communication involves more than a data interface, it is a separate entity from the host computer interface currently available on most instruments.
C INTELLIGENT SOFTWARE LINK (ISL) OPTION ISL OPTION FOR RALS E.
INDEX Symbols +24 V POWER ON signal location, 2.2-6 on +24 V power supply, 2.4-9 +24 V power supply +24 V POWER ON signal, 2.2-6, 2.4-9 adjustments, 2.2-6 description, 2.2-6 ERROR DETECTED (015) Power Supply Failure, 4.12-3, 7.4-3, C.2-1 ERROR DETECTED (027) Aspirate Syringe Failure, 7.4-1, C.2-1 fuses used, 2.2-6 installation procedures, 4.12-2 interaction with AC Power/Vacuum Relay card, 2.2-1 interaction with Power Supply Conditioning card, 2.5-7 location, 2.2-3, 4.4-1, 4.12-1 location of connections, 4.
INDEX Power On/Off switch, 2.2-1, 2.2-2 removal procedures, 4.5-1 replacement, 2.2-1 schematics, 2.2-1 table of output connectors, 2.2-1 tools/supplies needed for replacement, 4.5-1 verification procedures, 4.5-2 AT select switch switch settings, 3.2-1 attention definition, 1.1-2 Auto Print function description, 7.2-1 B Base 64 KB Memory Failure description, C.3-1 beep codes 1 Refresh Failure, C.3-1 10 CMOS Shutdown Register Read/Write Error, C.3-1 2 Parity Error, C.3-1 3 Base 64 KB Memory F, C.
INDEX D Data Acquisition section functional relationship, 2.1-1 Diagnostic Data Acquisition circuit description, 2.4-4 diluent reservoir description, 2.3-7 location on Fluidics Panel, 2.3-1 Diluter Functions menu options CLEAN BATHS, 7.1-1 CLEAR APERTURES, 7.1-1 DISPENSE LYSE, 7.1-1 DRAIN, 7.1-1 MIX, 7.1-1 RINSE, 7.1-1 Diluter Panel aperture system, 2.3-2 components, 2.3-2 description, 2.3-2 fluidic solenoids, 2.3-1, 2.3-4 Hgb LED, 2.3-2 interaction with Sensor Preamp Adapter card, 2.
INDEX E electronic signals +24 V POWER ON signal, 2.2-6, 2.4-9 HEMOGLOBIN signal, 2.5-3 HGB VOLTAGE (+5 V), 2.4-4 LYSE PUMP signal, 2.4-9 PLT PREAMP signal, 2.5-9 PLT signal, 2.5-2 POWERFAIL (PF/PG) signal, 2.4-8 POWERFAIL signal, 2.2-6, 2.4-10, 7.4-3 RBC 26-PERCENTILE VOLTAGE, 2.4-4 RBC APERTURE VOLTAGE, 2.4-4 RBC PREAMP signal, 2.5-9 RBC signal, 2.5-2 RBC/PLT APERTURE signal, 2.5-1 VAC ON signal, 2.2-2, 2.2-5 VACUUM READING (+12 V), 2.4-4 VACUUM SENSOR signal, 2.4-5 WBC 26-PERCENTILE VOLTAGE (+15 V), 2.
INDEX F filters PMI recommendations, 5.2-1 Flex Connect card function, 2.3-7 interaction with Probe/Wipe Traverse Assembly, 2.3-7 LED power indicator, 2.3-7 location, 2.2-3, 4.4-1 part number, 8.1-4, 8.2-3 solenoids, 2.3-7 table of connectors, solenoids, LEDs, 2.3-7 floppy drive description, 2.6-1 ERROR DETECTED (018) Copy Protection Violation, 7.4-1, C.2-1 location, 2.2-3 part number, 8.1-6, 8.2-4 verify operation, 4.6-4 fluid reservoir Fluidics Panel, 2.3-7 fluidic solenoids Diluter Panel, 2.3-1, 2.
INDEX K keypad 8279 keyboard controller chip, 2.4-3 description, 2.6-1 input connector, 2.4-5 installation procedures, 4.10-2 interaction with URA card, 2.4-2 location, 2.2-3, 4.10-1 location of connectors, 4.10-1 part number, 8.1-6, 8.2-2 removal procedures, 4.10-1 replacement, 2.6-1 Software Fatal Error, 7.4-2, 7.4-4 tools/supplies needed for replacement, 4.10-1 verification procedures, 4.10-2 verify operation, 4.8-2 keypad controller description, 2.4-3 Keypad Failure, 7.4-2 L LATEX GAIN description, 7.
INDEX table of input connectors, 2.4-10 table of jumper settings, 2.4-11, A.2-3 table of output connectors, 2.4-10 table of test points, 2.4-11, A.2-3 table showing jumper settings, A.2-3 tools/supplies needed, 4.15-1 unpacking the instrument, 3.2-1 verification procedures, 4.15-2 motors DRA card, 2.4-6, 2.4-7 interaction with DRA card, 7.4-2 Motor/Solenoid Driver card, 2.4-9 peristaltic pump motors, 4.22-2 probe motor, 2.3-4, 2.4-9 stepper motor, 2.3-4, 2.3-6, 2.4-6, 2.4-7 Syringe Assembly, 4.
INDEX table showing connector, power ranges, part numbers, 3.1-2 power supplies +24 V power supply, 2.2-6 AT power supply, 2.2-1 description, 2.2-1 ERROR DETECTED (014) Power Supply Failure, 7.4-2, C.2-1 ERROR DETECTED (015) Power Supply Failure, 4.12-3, 7.4-3, C.2-1 fuses used, 2.2-1 input/output voltage, 2.2-1 interaction with DRA card, 2.2-1 interaction with Linear Power Supply card, 2.2-1 interaction with Motor/Solenoid Driver card, 2.2-1 Linear Power Supply card, 2.
INDEX connecting the Printer, 3.2-3 connecting the reagents, 3.2-2 entering the initial instrument settings, 3.2-4 Hgb preamp adjustment, 4.29-1 latex gain adjustment, 2.2-7, 4.27-1, 7.1-2 lyse volume adjustment/verification, 7.1-1 opening the lower chassis, 4.4-1 rebooting the system, 4.2-2 removing the top cover, 4.3-1 Service Diagnostic, 7.3-1 starting the instrument, 3.3-1 system verification, 5.1-1 unpacking the instrument, 3.2-1 vacuum adjustment, 4.14-2, 4.23-1, 4.
INDEX Serial 2 connector function, 2.6-1 table of pinouts, 2.6-1 serial ports Serial 1, 2.6-1 Serial 2, 2.6-1 table of pinouts, 2.6-1 verify operation of port, 4.6-4 Service Diagnostic description, 7.1-2, 7.3-1 Diluter Panel solenoids, 4.25-1 diluter table, 2.1-2 interaction with Vacuum Sensor card, 7.3-1 Motor/Solenoid Driver card, 4.15-3 procedures, 7.3-1 table showing cycle, 7.3-1 verify operation of solenoid valves, 4.9-2 Service menu options LATEX GAIN, 7.1-2 PULSE TEST, 7.1-2 REPLACE SYRINGE, 7.
INDEX SVP, 5.1-1 sweep-flow system Diluter Panel, 2.3-2 sweep-flow tubing installation procedures, 4.26-2 interaction with Diluter Panel, 4.26-1 location, 4.26-1 location of components, 4.26-1 part number, 4.26-2 removal procedures, 4.26-1 tools/supplies needed for replacement, 4.26-1 verification procedures, 4.26-2 switch settings Ac Select switch, 3.2-1 AT motherboard, 2.4-1, A.2-1 URA card, 2.4-6, A.2-4 switches Ac Select switch, 2.2-2 instrument’s power ON/OFF switch, 2.2-1, 2.
INDEX ERROR DETECTED (009) URA Board Failure, 7.4-5, C.2-1 ERROR DETECTED (011) URA CMOS Failure, 7.4-5, C.2-1 ERROR DETECTED (012) System Disk File Corrupt, 7.4-5, C.2-1 ERROR DETECTED (016) System Disk File Corrupt, 7.4-4 input/output voltage, 2.4-4 installation procedures, 4.8-2 jumper and switch settings, 4.8-2 jumper location and setting, 2.4-5, 4.9-1 keypad controller, 2.4-4 location, 2.2-3, 4.6-1, 4.8-2 part number, 8.1-6, 8.2-4 removal procedures, 4.8-1 Software Fatal Error, 7.4-2, 7.
C TRADEMARKS “CC” logo, COULTER, COULTER CLENZ and 4C are trademarks of Coulter International Corp. Centronics is a registered trademark of Centronics Data Computer Corporation. CITIZEN is a registered trademark of Citizen America Corporation. DOW CORNING 33 is a registered trademark of Dow Corning Corporation. Epson is a registered trademark of Epson America, Inc. FLUKE is a registered trademark of John Fluke Mfg. Co., Inc. IBM is a registered trademark of International Business Machines, Inc.
