SemenView For Program Version 1.
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SemenView ™ A part of the NucleoCounter ® SP-100™system Manual No. 991-0103 (English) User’s Guide Version 1.3 May 25, 2005 ChemoMetec A/S Gydevang 43, DK-3450 Allerød Telephone: +45 48 13 10 20, Fax: +45 48 13 10 21 Internet: www.chemometec.com, E-mail: info@nucleocounter.dk or info@chemometec.
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Customer support If you have questions about installing or using your SemenView T M software check this user's guide first - you will find answers to most of your questions here. If you need further assistance, please contact customer support at ChemoMetec A/S.
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Intended use Intended use The SemenView™ software is intended to be used with the NucleoCounter ® SP-100™ instrument, for the purpose of semen analysis; it is intuitive and easy to use. SemenView™ offers the user several features when connected to the NucleoCounter ® SP-100™. SemenView™ is intended for the documentation of results from the NucleoCounter ® SP100™, results can be printed or saved for later use using the SemenView application.
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Table of contents Table of contents 1 INSTALLATION ..................................................................................................1 1.1 I NSTALLATION OF THE 1.2 I NSTALLATION OF 1.3 R EMOVAL 3 4 5 6 7 DRIVER - W INDOWS 2000 ...................................................... 1 S EMEN V IEW TM – W INDOWS 2000 ......................................................... 4 S EMEN V IEW SOFTWARE ....................................................................... 7 1.3.
Table of contents 7.1 8 S EMEN V IEW ™ SOFTWARE ........................................................................................ 33 APPENDIX 1: PRINTED REPORT LIST .................................................................. 35 9 APPENDIX 2: INSTALLATION OF THE USB DRIVER ON OTHER WINDOWS OPERATING SYSTEMS. ............................................................................................................ 37 10 INDEX ...............................................................
1 Installation 1 Installation When the NucleoCounter SP-100 is connected to a computer running SemenView it is possible to register the estimated cell concentration and the collected image of the SP1Cassette chamber. Connecting a NucleoCounter SP-100 to a computer does not affect the operation of the NucleoCounter SP-100 in any way and the SemenView software performs no additional image analysis.
1 Installation Figure 1 The Found New Hardware Wizard. The driver needed is supplied on the NucleoCounter SP-100 CD-ROM. Insert the CD-ROM and click “Next”. The next dialogue is shown in figure 2. Figure 2 Search for a driver for the NucleoCounter SP-100. Select the default method “Search for a suitable driver for my device” and click “Next” to continue. The dialogue shown in figure 3 will appear.
1 Installation Figure 3 Locations where the computer searches for the driver for the NucleoCounter SP-100. Make sure that the “CD-ROM drives” is selected as “Optional search location” and click “Next” to continue. Figure 4 The driver has been found on the NucleoCounter SP-100 CD-ROM. Verify that the wizard has found “nucleo.inf” on the NucleoCounter SP-100 CD-ROM and click “Next” to continue, see figure 4. If “nucleo.
1 Installation Figure 5 The installation has been successful. If the installation was successful the dialogue in figure 5 will appear. Click “Finish” to complete the installation. Now the USB device driver for the NucleoCounter SP-100 is successfully installed. It will not be necessary to redo the installation unless you wish to use the NucleoCounter SP-100 on another computer or if you reinstall Windows 2000. 1.
1 Installation For the installation of SemenView insert the NucleoCounter SP-100 CD-ROM into the computer. To start the installation, locate the file “SETUP.EXE” on the CD-ROM and open it (double-click it using the mouse). The dialogue shown in figure 6 will appear. Click “Finish” to complete the installation. Figure 6 Dialogue box for installation of SemenView. When the installation is finished the computer will display a dialogue saying “SemenView installation successful!”.
1 Installation In the next dialogue Figure 8, please confirm the default destination folder for installation of the LabVIEW Run-Time Engine by clicking “Next”. Figure 8 Dialogue for the selection of the destination folder. Please select the default folder by clicking “Next” The dialogue shown in figure 9 finally allows you to implement a modification of the LabVIEW application. Click “Next” to continue or click “Back” to check your selection for the installation.
1 Installation If the installation is successful the dialogue shown in figure 10 will appear. Click “Finish” to complete the installation. The SemenView software and LabVIEW application are now installed. Figure 10 Successful installation of the LabView application. For directions on start-up and use of the SemenView software please refer to chapter 2 Getting started with SemenView TM on page 11. 1.
1 Installation Figure 11 “System Properties” dialogue. In the “System Properties” dialogue select the “Hardware” tag (figure 11) and click on the “Device Manager” button. A list of system devices will be shown, see figure 12. to the left of the name. This Locate “nucleo Device” and open it by clicking on the will show its content, which also has the name “nucleo Device”. If “nucleo Device” is not on the list, make sure that the NucleoCounter SP-100 is turned On and connected to the computer and try again.
1 Installation Highlight the “nucleo Device” as shown in figure 12 and option-click it using the right mouse button. This activates a pop-up menu. Select the “Uninstall…” function and the USB device driver will be removed from the system. 1.3.2 Removal of SemenView TM - Windows 2000 To remove the SemenView software 3, open the SemenView folder (Figure 13). The folder should be located in the root directory of the local hard disk (C:\SemenView) if the location was not changed during the installation.
1 Installation The SemenView software and LabVIEW application will then be removed from your computer. Any saved data will remain on the computer. 1.4 Upgrade the SemenView software For upgrading the SemenView software the previously installed version must be removed before the new version can be installed. Follow the directions from 1.3.2 Removal of SemenView TM - Windows 2000. The uninstall procedure will not interfere with previously stored data.
2 Getting started with SemenView TM 2 Getting started with SemenView T M SemenView allows the user to register the results of counting of cells in Semen samples with the NucleoCounter SP-100 on a computer. This is useful when documentation of results and data is needed. 2.1 Launch SemenView TM SemenView can be activated through the Windows “Start” menu. First select “SemenView” from the “Programs” submenu, and then select “SemenView”.
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3 The structure of SemenView TM 3 The structure of SemenView T M NucleoView is operated through a number of tabs. The different tabs are selected by clicking on the corresponding tab. The tabs are shown in figure 17. Figure 17 The three tabs of SemenView. The default startup screen is the Analysis tab. For detailed descriptions of the different tabs see the following chapters. An overview of the three different tabs is given below. 3.
3 The structure of SemenView TM 3.2 Utility tab Here it is possible to print data from a chosen session (folder), select language, set preferred text editor and set a sound scheme when a new result is present in SemenView. 3.3 About tab This tab displays version information of the SemenView program. No further presentation of this tab will be made.
4 Analysis tab 4 Analysis tab Analysis is the default start-up tab of SemenView. In this tab data and results transferred from the NucleoCounter SP-100 are automatically displayed. An example of the Analysis tab is shown in figure 18. Once SemenView has been launched the Analysis tab is automatically displayed. Usually there are no files in the active session upon start-up. Start the measurements or use the Browse button to navigate to another session (see chapter 4.6 Folder selection.).
4 Analysis tab 4.2 Retest of sample If the NucleoCounter SP-100 suggest to perform a retest of the sample, then SemenView will show a flashing Retest indicator and the digits will turn red in the result field. Figure 19 The digits turns red and the Retest indicator will flash red if Retest of a sample is suggested In some cases the following is displayed in the result field: Figure 20 No digits are shown – only red dashes, and the Retest indicator will flash red if the instrument reports an error.
4 Analysis tab Figure 21 Dilution factor (DF), Sample ID and Setup 4.4 Sample ID – Sample identification During analysis on the NucleoCounter SP-100 a numerical Sample ID can be entered. This number will if entered be displayed in the field below the Dilution Factor. 4.5 Setup – Measurement setup The NucleoCounter SP-100 provides analysis of different species of animal. Once a Measurement setup is activated it will be shown in the "Setup" field. E.g. Boar, Bull etc. 4.6 Folder selection.
4 Analysis tab Figure 23 The file list made of filename and analysis result. It is possible to browse through files or results from the active session. To select and view a file from the list use the mouse or the arrow keys on the keyboard. The selected file will be displayed including all data. To browse files from other sessions, follow the directions below. 1. Click the “Folder…” button. This activates a dialogue where another session, which is to become the active session, can be located. 2.
4 Analysis tab marked the SemenView program will find the file from the first valid Total analysis listed before (above) the Non-viable file selected, from the file list. The Viability is calculated as: Viability = 100 ⋅ Total − Nonviable % Total Please refer to your NucleoCounter SP-100 User's Guide for reference on how to perform Viability analysis. In the case presented here the Viability becomes: Viability = 100 ⋅ 545.1mill − 137.4 mill % = 75 % 545.
4 Analysis tab The NucleoCounter SP-100 records the image of the SP1-Cassette chamber when counting cells. The image is then processed in the build-in computer of the NucleoCounter SP-100 to give an estimate of the cell concentration, counting every star or white dot as a nucleus. Figure 26 SemenView presents the images from the NucleoCounter SP-100 like a starry sky. Each “star” represents a stained nucleus.
5 Utility tab 5 Utility tab In the Utility tab (figure 27) it is possible to print and save results. Furthermore it is possible to select language, text editor and sound scheme. Figure 27 The Utility tab. 5.1 Print results The report path is found in the top of the Utility tab. Please note that printing only can take place if a valid printer is configured in the Printers folder of the Windows 2000 Control Panel.
5 Utility tab If no folder is selected SemenView suggest that the folder from today is selected. Figure 29 Accept selection of today's folder Using the button to the right in Figure 28 any folder can be selected as the basis of a report. Once activated, locate the folder and click “Select Cur Dir”. The file that is printed is also saved on the hard disk of the PC (see Figure 30). If a report has already been created and stored, the new report will overwrite the older report.
5 Utility tab Figure 31 Correction factor 5.3 Setting the language of SemenView. A list box shows the possible languages for SemenView. Figure 32 Select language for SemenView ChemoMetec A/S will implement new languages. Please contact ChemoMetec A/S NucleoCounter SP-100 Technical Support on the address info@nucleocounter.com for any update on available languages. 5.
5 Utility tab Figure 34 Select a text editor If WordPad is highlighted in the text editor list box it must be located on the hard disk. This will be prompted (Figure 35) for the first time a report will be printed. SemenView knows the location of Notepad. If the location of WordPad is prompted for then follow the directions below to locate its position. SemenView will remember the information of WordPad's location. Figure 35 Locate WordPad 1. The dialogue shown in figure 36 appears. Locate “wordpad.
5 Utility tab Figure 36 This dialogue appears when WordPad is configured as text editor. 5.5.1 Define font for WordPad To create reports with results in columns the font for the text editor must be defined. This is done differently for WordPad and Notepad. Follow the directions below to define a font for WordPad. 1. Open WordPad and select “Save as” from the File menu. The dialogue shown in figure 37 appears. 2. Choose the location to save the file to be the SemenView folder (usually C:\SemenView). 3.
5 Utility tab Figure 37 The dialogue shown when setting up the font for WordPad. 6. After pressing “Save” the dialogue shown in figure 38 appears. Press “Yes” and the font for WordPad is defined. Now WordPad will by default use the font Courier, size 10 regular. Figure 38 Confirm the setup for WordPad by pressing, “Yes”. 7. Exit WordPad. 5.5.2 Define font for Notepad Similarly Notepad can be set up for SemenView, follow the directions below. 1. Open Notepad. 2. Choose “Font…” through the Format menu.
5 Utility tab Figure 39 Defining the font for Notepad. 3. Choose the font Courier, Font style Regular and Size 10 as in figure 39. Now Press OK and exit Notepad. Notepad will use the font Courier, size 10 regular.
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6 Troubleshooting 6 Troubleshooting Occasionally difficulties with the SemenView software may occur. In the following a short description and suggested action is given. 6.1 Quality of the sample Through the CM file image it is possible visually to assess the quality of the sample preparation and thereby the reliability of the measurement by looking at the “starry sky”. The measurement is reliable if the stars or white dots are distributed separately and evenly over the image.
6 Troubleshooting Figure 41 Input open failure Figure 42 Input opened If "Input open failure" is stated in the Info field, check the USB cable. Alternative, if the NucleoCounter SP-100 is turned off, “Input open failure” will also appear in the Info field. If data still is not received quit SemenView and close the NC Receiver. Then open SemenView and check that the NC Receiver is also started. If necessary, restart the computer before opening SemenView again.
6 Troubleshooting SemenView is activated. If the “QUIT”-button does not respond (after only few seconds), you can close SemenView down using the Windows Task Manager. For instructions on the use of Windows Task Manager please refer to the documentation supplied with Windows. Low resources on the PC running SemenView can cause the result field to show the following message "Not SP-100". You can check your resources on the PC using the Windows Task Manager.
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7 Technical specifications 7 Technical specifications 7.1 SemenView™ software System requirements For operating systems see table 1 below. Soundcard and loudspeakers. USB 1.1 port 8. Display Screen Area: Minimum 1024 x 768 9. Before installation of SemenView the USB driver for the NucleoCounter SP-100 must be installed (see chapter 1.1 Installation of the USB driver - Windows 2000). Table 1 Functionality of SemenView and the USB driver on computers running different operating systems.
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8 Appendix 1: Printed report list 8 Appendix 1: Printed report list Below is an example of what the printed and saved report list looks like when it is generated. Sample ID 173 258 0997 - Cells/ml Sample 496.2 mill. 0.000 mill. 491.6 mill. 462.7 mill. 570.8 mill. 525.4 mill. 556.3 mill. 550.3 mill. 1128 mill. 1063 mill. 1150 mill. 1705 mill. T 363.0 mill. N 342.9 mill. T 348.3 mill. T 362.2 mill. T 362.2 mill. T 743.7 mill. T 700.0 mill. N 1060 mill. T 112.5 mill. N 117.5 mill. 106.1 mill. 132.2 mill.
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9 Appendix 2: Installation of the USB driver on other Windows operating systems. 9 Appendix 2: Installation of the USB driver on other Windows operating systems. Installation Windows XP UK Installation of the USB driver on a PC with the Windows XP operating system Ensure the computer is switched on, The NucleoCounter properly connected and the Installation CD-ROM is loaded into appropriate drive.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Then Windows XP will install the USB driver. This can take a minute or so, and during the installation a message like the following is displayed. Clicking “Finish” completes the installation. Note: The NucleoCounter USB driver does not support all types of USB hubs. Please be advised that some computers feature internal hubs, and the NucleoCounter system may not function under those circumstances.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. This Technical Note assumes that the operating system is intended for and ready for the use of USB Rev 1.1 communication ports. Application Program Installing and using the Application program: The installation, and the use of the Application program will appear as described in the manual. The only change will be that the Windows XP operating system has dialog boxes, windows etc. with different appearance. E.g.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Installation Windows 98 UK 2 nd edition Installation of the USB driver on a PC with the Windows 98 UK 2 nd edition (Windows 98) operating system Ensure the computer is switched on, the NucleoCounter connected to the PC and the Installation CD-ROM is loaded into a CD-ROM drive.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Select the option ”Display a list of all the drivers in a specific location, so you can select the driver you want.” and hit “Next”. Chose ”Other devices”, and click “Next”.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. The USB Driver for the NucleoCounter is not supported by Windows 98, therefore you must tell the system where to find the software for it by clicking “Have Disk…” Click on ”Browse…”, and chose the path for the CD-ROM drive having the Installation CD-ROM.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Select the “nucleo.inf” file and click “OK” and click “OK” once more Information about the The USB Driver for the NucleoCounter is now present for Windows 98, click “Next” to proceed.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Accept the location of the USB driver by clicking “Next” Windows 98 now copies files and installs the USB driver.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Clicking “Finish” completes the installation. Note: The NucleoCounter USB driver does not support all types of USB hubs. Please be advised that some computers feature internal hubs, and the NucleoCounter system may not function under those circumstances. This Technical Note assumes that the operating system is intended for and ready for the use of USB Rev 1.1 communication ports.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Removal (uninstallation) of the USB Driver Windows XP UK Ensure the computer is switched on and the NucleoCounter properly connected, then use the mouse and right click on the “My Computer” icon, and chose “Properties”. Select the “Hardware” tab, and click on the “Device Manager” button.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. On the “Device Manager” list open the “nucleo Device” list, then right click on the “nucleo Device” item, and select “Uninstall”.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. The “nucleo Device” is no longer visible on the “Device Manager” list and the USB Driver is now successfully uninstalled from the system. Note: This process only removes the “nucleo Device” from the “Device Manager” list, the system files for the USB driver are still present on the PC.
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Removal (uninstallation) of the USB Driver Windows 98 UK Ensure the computer is switched on and the NucleoCounter properly connected, then use the mouse and right click on the “My Computer” icon, and chose properties. In the “System Properties” dialog box Select the “Device Manager” tab..
9 Appendix 2: Installation of the USB driver on other Windows operating systems. Confirm removal by clicking “OK” The “nucleo Device” is no longer visible on the “Device Manager” list and the USB Driver is now successfully uninstalled from the system. Note: This process only removes the “nucleo Device” from the “Device Manager” list, the system files for the USB driver are still present on the PC.
10 Index A Analysis tab ...........................................15 B Browse files from active session ...........18 Browse stored files ................................18 N NC Receiver ...........................................11 nucleo Device ..........................................8 nucleo.inf .................................................3 O C Operating systems .................................33 Collected image .....................................19 Correction factor ...................
