Quick Guide Viability & Cell Count, Cell Health & Cell Cycle in one instrument! NucleoCounter ® NC-250 ™
How to get started Dear NucleoCounter Customer! Thank you for purchasing the NucleoCounter® NC-250™ offering not only easy Viability and Cell Count Assays, but also fast and easy assaying of cell cycle profiles with top class precision and the fastest apoptosis assay! The NucleoCounter® NC-250™ Concept 8 viability and cell counts in less than 3 minutes - Total cell count - Viability - Cell diameter - % of cells in clumps Fast and easy operation Excellent reproducibility No calibration Main
How to Perform Viability and Cell Count Analysis Fast and Easy Cell Count and Viability Assays! The NucleoCounter® NC-250™ from ChemoMetec is a significant step forward for automated cell counting.
Optional: Visual Inspection of Counting Gates OPTION Click the green dot in the right-hand corner of the image window. This activates the image overlay function indicating the events included in the total cell count. The mouse scroll button allows the user to zoom in at the cursor position. Cells will be framed by a pink square. (PREFERRED) OPTION (ADVANCED) Right-click on the sample file name in the ’Data folders and files’ window and select ’Show counting gates in Plot Manager’.
How to Perform a 2-Step Cell Cycle Analysis The NucleoCounter® NC-250™ provides the world’s fastest assaying of cell cycle profiles with top class precision.
How to Perform a Cell Health Analysis The NucleoCounter® NC-250™ provides cell health measurements by the world’s fastest apoptosis assay. VitaBright-48™ a componentof Solution 6 instantly fluoresces when it binds thiols inside cells. The level of thiols is a direct measurement of cell vitality and a drop in the thiol level is concomitant with apoptosis.
Only If Required: Create your own version of a Protocol with Adjusted Generic Gates Perform the desired type of assay on a sample of the cells to be analysed. In the Tools menu select Protocol Adaptation Wizard. Follow the instructions in the Protocol Adaptation Wizard to create your own adapted protocol. The viability and cell counts results will not be adjusted for the image file used for the adaption of counting gates.
Additional Resources Go to www.chemometec.com to find: • • • • • Documentation MSDS Application notes Certificates of analysis Webinars etc. Consumables/kits: Item no.
