Specifications

9
High Performance Liquid Chromatograph
Rack changer II (228-45164-XX)
Compatible plates: 96-well MTPs, 96-well DWPs
384-well MTPs, 384-well DWPs
1.5 mL vial plate (54 vials)
Number of processed plates: 12
Sample cooler: Block cooling/heating, used together with
dehumidifying function, 4 to 40°C
A rack changer is an optional product that can be used to change the microplates in the
autosampler’s racks and thereby facilitate serial analysis. Up to 12 plates can be mounted
in the rack changer.
This model incorporates a cooling function. When the samples have been prepared, simply
set them in the rack changer to perform continuous sample processing.
The SIL-20AHT / 20ACHT autosamplers used
with the Prominence UFLC system permit
ultra-fast analysis while maintaining the
injection accuracy and durability
requirements demanded of an HPLC system.
As shown in the right, the retention time
reproducibility and injection volume
reproducibility are within 0.3 % after
endurance testing over 100,000 cycles,
results similar to those achieved with
standard HPLC.
Repeatability for 4 Alkyl Phenones After Finishing 100,000 Cycle Endurance Test
Retention Time
Average
0.916 0.089 312,670 0.059
315,739 0.045
336,428 0.063
296,609 0.040
0.058
0.030
0.031
1.398
2.111
3.341
%RSD Average %RSD
Area
Components
Acetophenone
Propiophenone
Butyrophenone
Valerophenone
(5 µL injection, n=6)
Rack Changer: Increasing the Number of Processed Samples
Prominence UFLC Offers Ultra Fast HPLC Analysis
−10
0
50
100
150
200
240
mAU
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 min
Combination with LCMS for Ultra Fast Analysis
Combining Prominence UFLC with a
Shimadzu mass spectrometer, which
features ultra-fast polarity switching
between positive/negative ion modes
(UFswitching), superb sensitivity due to the
newly-developed Qarray
™
ion optical system
(UFsensitivity), and an ultra-fast scanning
speed (UFscanning), enables ultra-fast
analyses that produce peak widths in just a
few seconds and highly reliable data.
Ultra-High-Speed Analysis of 6 Pharmaceutical Ingredients
1
2
3
4
5
6
0.0 0.5 1.0 1.5 min
TIC (Positive)
TIC (Negative) × 5
m/z 355 (Positive)
m/z 250 (Positive)
m/z 265 (Positive)
m/z 231 (Positive)
m/z 321 (Negative) × 50
m/z 329 (Negative) × 50
Detection
Column
Mobile phase
Flow rate
Temperature
Injection volume
Observed analysis
column pressure
: ESI-Positive mode; LCMS-2020
: Shim-pack XR-ODS (30 mmL. × 3.0 mmI.D.)
: A) 0.1% aqueous solution of formic acid
B) Acetonitrile
B.Conc.: 20% (0 min.) 35% (2.0 min.)
: 0.5 mL/min.
: 50°C
: 1 µL
: 14 MPa
Peaks
1: Yohimbine, [M+H]
+
m/z 355
2: Alprenolol, [M+H]
+
m/z 250
3: Tetracaine, [M+H]
+
m/z 265
4: Chloramphenicol, [M−H]
−
m/z 321
5: Furosemide, [M−H]
−
m/z 329
6: Isopropylantipyrine, [M+H]
+
m/z 231