Specifications

7
High Performance Liquid Chromatograph
The preparative LC system supports both analysis and fractionation (including gradient analysis and
fractionation), which permits efficient scaling up with a single instrument and single operating environment.
The solvent delivery unit LC-20AR can handle flow rates ranging from those used in accurate analytical scale
to those used in semi-preparative. It enables semi-preparative recycling by using a recycle kit.
The high solvent delivery performance of the LC-20AP permits an accurate and efficient environment for
scaling up, in both the preparative and analytical flow rate ranges.
The LC-20AP Quaternary which is combined the LC-20AP with a dedicated FCV-200AL low-pressure gradient
unit, allows gradient fractionation to be performed in a single unit, thereby saving equipment costs.
LC-20AR / 20AP
Supporting from Analytical to Preparative Scales
Supports from Parameter
Investigation to Scaling Up
Typical values of the total component weight for a single injection
performed by a 250 mm column, where the target component is
(1) highly soluble in the mobile phase, (2) separated from
contaminating components, and (3) subjected to ion suppression,
are indicated on the right. With isocratic elution, these values are
basically proportional to the volume of the column.
Typical Maximum Load (Single Injection, Column Length of 25 cm)
Flow Rate (mL/min)
3 mg
3 mg
3 mg 20 mg
20 mg
300 mg
300 mg
2 g
2 g
20 mg 300 mg 2 g
Semi-micro
Semi-micro
Analysis
Analysis
Semi-preparative
Semi-preparative
Preparative
Preparative
2 mmI.D.
2 mmI.D.
5 mmI.D.
5 mmI.D.
20 mmI.D.
20 mmI.D.
50 mmI.D.
50 mmI.D.
2 mmI.D.
0.001
0.001
0.01
0.01
0.1
0.1 1
10
10
100
100
0.001 0.01 0.1 1 10 100
5 mmI.D. 20 mmI.D. 50 mmI.D.
10 mL/min
10 mL/min
10 mL/min
20 mL/min
20 mL/min
20 mL/min
150 mL/min
Semi-micro Analytical Semi-preparative Preparative
LC-20AT
LC-20AR
LC-20AP
High-Precision Delivery Performance
A redesigned plunger actuation mechanism and improved pulse
correction and check valves offers significantly improved flow rate
accuracy and flow rate precision. High retention time
reproducibility for analytical flow rates improves reliability when
scaling up and verifying purity.
Samples
(retention time
at %RSD n = 6)
1: Methylparaben
(0.015%RSD)
2: Ethylparaben
(0.011%RSD)
3: Propylparaben
(0.008%RSD)
4: Butylparaben
(0.010%RSD)
Flow rate: 1.0 mL/min
1200
mV
1000
800
600
400
200
0
1 10 min98765432
1
2
3
4
Retention Time Reproducibility for Analytical Flow Rates
LC-20AP Quaternary Enables Fractionation as a Low-Pressure Gradient System
25.0 min
mAU
20.015.010.05.0
0.0
0
500
1000
1500
Rosmarinic acid
Carnosol Carnosic acid
Column
Mobile phases
Gradient program
Column temperature
Injection volume
Flow rate
Detection
Sample
Shim-pack PREP-ODS 250 mmL. × 20 mmI.D., 15 µm
A: Water B: Methanol C: 2 % aqueous formic acid solution
B. Conc. 30 % (0 min) to 95 % (15 to 30 min) C. Conc. 5 %
Room temperature
200 µL
20.0 mL/min
UV 230 nm
Rosemary extract
1
5
µ
m
c
id solutio
n
Conc. 5
%
Degassing Units DGU-20A3R / 20A5R
The DGU-20A3R/20A5R is an on-line degassing unit that uses fluoroethylene membrane. The internal
capacity is small at 0.4 mL, only 1/25th of that for existing Shimadzu models, and the waiting time at
mobile-phase replacement or stabilization can be significantly reduced. The degassing efficiency has
also been improved, ensuring thorough degassing even at high flow rates.
Number of degassed solvents for DGU-20A3R : 3
Number of degassed solvents for DGU-20A
5R : 5
The LC-20AP Quaternary flow rate range is 1 to 50 mL/min and the instrument can handle up to four mobile phases simultaneously.
It considerably reduces the effort required to investigate mobile phases during method development.