Specifications

25
High Performance Liquid Chromatograph
Column:
Mobile phase:
Flow rate:
Temp.:
Sample:
Shim-pack VP-ODS
(150 mmL. ×
4.6 mmI.D.)
Water/Methanol
Gradient (60/40–
40/60, 2%/min)
1 mL/min
40°C
PEG-1000
Light Source
Assist Gas Assist Gas
Photomultiplier
*1: Requires gas supply source, such as a gas line, nitrogen generator, or air
compressor.
• An optional pressure regulator with filter is required to remove microscopic materials
in gas.
• When using a nitrogen generator or an air compressor, please be careful that
moisture, oil, dust, etc. should not be contained in nitrogen or air.
• Please use it in the room where exhaust equipment is available.
Note
Evaporative Light-Scattering Detector
ELSD-LT ll
Nebulizing Method
Light source
Detection
Temperature setting range
Nebulizer gas
Gas flow rate, Gas pressure
Mobile phase flow rate
Analog output
Operating temperature range
Operating humidity range
Size, Weight
Power supply
Specifications
ELSD-LT II (228-45115-xx)
Siphon Splitting
LED
Photomultiplier Tube
Ambient to 80°C
Nitrogen (N
2
) or Air
*1
Max. 3.0 L/min, Max. 450 kPa
0.2 to 2.5 mL/min
0 to 1 V
5 to 40°C
Max. 80% (5 to 31°C, room temperature)
Max. 50% (31 to 40°C, room temperature)
W250 × D550 × H450 mm, 20 kg
AC 115 V, 230 V, 150 VA, 50/60 Hz
The ELSD-LT II detector uses a unique nebulizer and drift tube design to achieve stable and
low-temperature evaporation of mobile phases, making it possible to analyze semi-volatile and/or
thermally unstable compounds.
High-sensitivity detection is achieved by focusing the sample at the detection point with assist gas
flow. The ELSD-LT II offers high sensitivity with this low-temperature evaporation technology and
superb detection technology. A smaller volume nebulizer and drift tube further improve sensitivity.
Assist Gas Functions
Example of analyzing 4 semi-volatile alkyl parabens,
considered difficult to analyze with conventional ELSD detectors.
0
50
100
150
200
250
300
350
mV
0.00 0.25 0.50 0.75 1.00 1.25 1.50
min
1
2
3
4
1: Methylparaben
(S/N = 101)
2: Ethylparaben
(S/N = 146)
3: Propylparaben
(S/N = 276)
4: Butylparaben
(S/N = 337)
With the exception of some highly volatile compounds, the ELSD-LT II is able to detect almost any
compound. Unlike traditional absorbance detectors (UV-Vis, PDA, etc.), sensitivity is not
dependent on the physical or structural properties of the compound, but rather the absolute
quantity of the solute passing through the detector cell. Therefore, it is especially useful for
detecting unknown or breakdown compounds and/or validating purity of a target compound. By
this mechanism of detection the ELSD-LT II is truly a universal detector.
Chromatogram of Non-Chromophoric Compound
Not all compounds have a chromophore or other such structural property that allows the use of an
absorbance detector. Refractive Index Detection (RID) is one option but it suffers from the inability to
run gradient analysis. Evaporative Light Scattering Detection (ELSD) is a perfect alternative to RID as it
is more rugged, quicker to stabilize, and gradient compatible. ELSD is ideal for applications like testing
the purity of compounds, measuring the molecular weight distribution of synthetic polymers, and
analyzing natural substances.
Highly Versatile Detection Method
Detects Most Compounds
High Sensitivity by Low Temperature Evaporation
Auto-Powerdown functions for the LED light source and nebulizer gas reduce operating costs. The
self-cleaning design makes maintenance of the drift tube easier.
Automated Functions
Analytical Conditions