Specifications

17
High Performance Liquid Chromatograph
Mobile phase
Flow rate
Column
Temperature
Detection
Hexane / 2-propanol = 100 / 0.5 (v/v)
1.0 mL/min (Conventional)
0.8 mL/min (UFLC)
Shim-pack CLC-SIL(M) (150 mmL. × 4.6 mmI.D., 5 µm: Conventional)
Shim-pack XR-SIL (75 mmL. × 3 mmI.D., 2.2 µm: UFLC)
30°C
298 nm excitation wavelength, 325 nm emission wavelength
Analysis Conditions
Fast response is required to follow the sharp peaks obtained in ultra fast LC analysis. The 10 ms response of the RF-20A/20Axs permits
ultra fast LC analysis with no loss of separation. In this analysis example, the analysis time was reduced by a factor of more than three,
while maintaining the separation.
Support for Ultra Fast Analysis
Switch from Conventional LC to Ultra Fast LC
Using detection at a single wavelength when performing
multicomponent simultaneous analysis of components with
different optimal detection wavelengths necessitates sacrificing
sensitivity for certain components.
The RF-20A/20Axs detectors eliminate this issue by incorporating a
four-wavelength measurement function that permits detection of
each component at the optimal wavelength.
Detection using wavelength switching in the left-hand diagram
exhibits incomplete separation in area (1) and one peak of reduced
size in area (2). In such a case, setting up to four optimal
wavelengths enhances the quantitative analysis accuracy by
reducing the effects of adjacent peaks and improving sensitivity.
Switching from Conventional LC to UFLC Analysis of α-, β-, γ-, δ-Tocopherols
Conventional LC (N for Peak δ = 11,708 )
1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min
1.00.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min
α
β
γ
δ
α
β
γ
δ
12.2 min
3.9 min
UFLC (N for Peak δ = 10,623)
Support for Improved Quantitative Analysis Accuracy
Utility of Four-Wavelength Measurement Function
The highly sensitive simultaneous analysis of multiple components requires detection at the optimal wavelengths.
The RF-20A/20Axs permit ultra fast, high-sensitivity multi-component analysis using wavelength switching by time program.
Multi-Component, High-Sensitivity UFLC Analysis
A
B
C
D
E
F
G
270 nm excitation wavelength, 330 nm emission wavelength
250 nm excitation wavelength, 370 nm emission wavelength
330 nm excitation wavelength, 430 nm emission wavelength
270 nm excitation wavelength, 390 nm emission wavelength
290 nm excitation wavelength, 430 nm emission wavelength
370 nm excitation wavelength, 460 nm emission wavelength
270 nm excitation wavelength, 330 nm emission wavelength
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min
A
BC D E F G
Naphthalene
Acenaphthene
Fluorene
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Benzo[a]anthracene
Chrysene
Benzo[b]fluoranthene
Benzo[k]fluoranthene
Benzo[a]pyrene
Dibenz[a,h]anthracene
Benzo[ghi]perylene
Indeno[1,2,3-cd]pyrene
Ultra Fast Simultaneous Analysis of 15 Polycyclic Aromatics
Analysis of Polycyclic Aromatics by Four-Wavelength Simultaneous Measurement (Elution sequence shown in previous diagram)
mV
6000
5000
4000
3000
2000
1000
0
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0
min
λ1 λ2 λ3
λ1: Ex = 260 nm, Em = 350 nm
λ2: Ex = 260 nm, Em = 420 nm
λ3: Ex = 305 nm, Em = 495 nm
(1)
(2)
mV
7000
6000
0.0
5000
4000
3000
2000
1000
0
2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0
min
1) Ex = 260 nm, Em = 420 nm
2) Ex = 285 nm, Em = 440 nm
3) Ex = 260 nm, Em = 350 nm
4) Ex = 305 nm, Em = 495 nm
(1)
(2)
Wavelength-Switching
Chromatogram
Four-Wavelength Simultaneous
Measurement Chromatogram