Specifications

15
High Performance Liquid Chromatograph
Mobile phase A: 0.1% aqueous solution of phosphoric acid
Mobile phase B: Acetonitrile
Flow rate: 1 mL/min
Column: Shim-pack VP-ODS 150 mmL. × 4.6 mmI.D.
* i-PDeA: Intelligent Peak Deconvolution Analysis
** i-DReC: Intelligent Dynamic Range Extension Calculator
A high-sensitivity cell with an 85 mm optical path length is available as an option for the SPD-M30A. It enables detection of trace
impurities that were conventionally difficult to detect and can be used for a wide range of analyses, from general analysis to ultra-fast and
UHPLC analysis.
The SPD-M30A high-sensitivity cell is also effective under HPLC conditions. The analysis below shows a comparison of measurements of
caffeine in a commercial soft drink using the SPD-M20A and SPD-M30A. It is apparent that the SPD-M30A improves the S/N ratio by up to
eleven times.
Impurity Analysis on Cefazolin Sodium (SPD-M30A)
Separation of Unseparated Peaks (i-PDeA*)
Extending the Dynamic Range (
i-DReC**)
: Standard cell (Optical path length: 10 mm)
: High-sensitivity cell (Optical path length: 85 mm)
This technique fully separates unseparated peaks and visualizes small
peaks hidden by a principal component. It is convenient when poor
separation occurs under conditions for accelerated analysis and for
quantitation of small peaks overlapped by a principal component peak.
See the separate technical reports for more details about i-PDeA and i-DReC.
This powerful technique permits quantitation of samples in the
high-concentration range. It enables the simultaneous analysis of
high- and ultra-low-concentration samples and the creation of
calibration curves across a broad concentration range.
: SPD-M20A (Standard cell)
: SPD-M30A (High-sensitivity cell)
1
2
3
4
5
SPD-M20A
(Standard cell)
2794
1527
16153
10894
10394
1
2
3
4
5
SPD-M30A
(High-sensitivity cell)
26814
15021
179070
118523
113870
S/N Relative Ratio
9.60
9.84
11.09
10.88
10.96
Signal Intensity
High-sensitivity cell Standard cell
49109
81339
16345
37922
7726
Impurity 1
Impurity 2
Impurity 3
Impurity 4
Impurity 5
7931
11438
2290
5548
968
New Analytical Techniques to Better Utilize PDA Detector Data
85 mm Optical Path Length Significantly Enhances Sensitivity
High-Sensitivity Cell (Option)
Area Area
Conc.Conc.