C196-E061U High-Performance Liquid Chromatograph Prominence
High Performance Liquid Chromatograph How HPL C Should Be High-performance liquid chromatography (HPLC) is widely used in diverse fields such as pharmaceuticals, and biochemistry to chemistry, the environment, and food products. The Shimadzu Prominence high-performance liquid chromatograph achieves an exceptional level of performance in each of these fields.
Superb Performance and Expandability Genuine High Throughput Reduced total analysis time Start 0 m 00 s The SIL-20A Series autosamplers achieve unparalleled speed, with a sample injection movement of just 10 seconds. Prominence reduces the total analysis cycle time, not simply the time for the HPLC analysis itself. The optional rack changer permits the serial analysis of up to 4,068 samples. Prominence is an HPLC system that offers genuine high throughput.
Hardware Expandability Flexibly accommodates customer needs from ultra-fast analysis to dedicated analysis systems Prominence allows configuration of the optimal analysis system for any application. The conventional HPLC is the typical system, and features simple operation. The UFLC system achieves a high level of separation at ultra-high speeds. The UFLC system and the LC/MS system maintain this ultra-high speed but offer higher separation performance.
Solvent Delivery Units LC-20AD Superior Solvent Delivery Performance The LC-20AD offers the fastest solvent delivery performance in the world. With an automatic pulsation-correction mechanism and high-speed micro plunger driving, it achieves pulse-free solvent delivery. Thanks to improvements in solvent-delivery control firmware, solvent-delivery performance in the micro-flow-rate range below 50 µL/min has been significantly improved.
LC-20AR / 20AP Supporting from Analytical to Preparative Scales The preparative LC system supports both analysis and fractionation (including gradient analysis and fractionation), which permits efficient scaling up with a single instrument and single operating environment. The solvent delivery unit LC-20AR can handle flow rates ranging from those used in accurate analytical scale to those used in semi-preparative. It enables semi-preparative recycling by using a recycle kit.
Autosamplers SIL-20A / 20AHT Supporting High-Throughput Analysis The SIL-20A is a total-volume injection-type Autosampler that enables high-speed injection and multi-sample processing. It was designed to ensure greater stability, with improved durability attained through modifications in valves and sample loops. SIL-20AC / 20ACHT Equipped with Cooling Function The SIL-20AC is equipped with a sample cooler that incorporates a dehumidifying function.
Rack Changer: Increasing the Number of Processed Samples A rack changer is an optional product that can be used to change the microplates in the autosampler’s racks and thereby facilitate serial analysis. Up to 12 plates can be mounted in the rack changer. This model incorporates a cooling function. When the samples have been prepared, simply set them in the rack changer to perform continuous sample processing.
Extension Modules A modular LC, Prominence can be combined with a system controller, column oven, and detector. This offers great flexibility; for example, a multi-oven function allows individual temperature control of multiple columns and simultaneous data acquisition using an absorbance detector and a fluorescence detector.
System Controllers Web Server Functionality Allows the HPLC System to Directly Create a Network. CBM-20A/20Alite is connected to a computer by Ethernet. The LAN cable connections allow the instrument and computer to be located in separated positions. Monitoring, simple operation control, and confirmation of the operation status can be performed on a mobile device.
Column Ovens The column oven precisely controls the temperature around the column to support stable analysis that is unaffected by changes in ambient temperature. It can contain various units and parts in addition to the column, including a manual injector (CTO-20A/20AC), gradient mixer, high-pressure flow-line selection valves (any two: 2-position/6-port, 6-position/7-port), cell block of an electric conductivity detector (CTO-20A/20AC), and a reaction coil (CTO-20A/20AC).
Analysis with Individual Temperature Control of Multiple Columns (Multi-Oven Function) The improved functions of the LabSolutions* workstation support up to four column ovens per system. This allows analysis with individual temperature control of two columns. mV 40°C temperature control 3 25.0 Unseparated 8 9 2 20.0 5 15.0 6 3 2 10.0 7 5.0 10 11 12 6 9 8 1 5.0 0.0 Improved separation 5 4 1 7 10 11 12 0.0 −5.0 −5.0 −10.0 10.0 mV 20.0 15.0 20.0 25.0 30.
UV-VIS Detectors / Photodiode Array Detectors The lineup includes SPD-20A/20AV dual-wavelength absorbance detectors and SPD-M20A/M30A photodiode array detectors. The SPD-M30A incorporates a new type of capillary cell to offer higher sensitivity and lower dispersion. All models offer temperature control for increased baseline stability with respect to temperature fluctuations and improved data reliability.
High-Sensitivity Cell (Option) A high-sensitivity cell with an 85 mm optical path length is available as an option for the SPD-M30A. It enables detection of trace impurities that were conventionally difficult to detect and can be used for a wide range of analyses, from general analysis to ultra-fast and UHPLC analysis.
Fluorescence Detectors The RF-20A/20Axs fluorescence detectors offer world-leading sensitivity* and ease-of-maintenance. The RF-20Axs is a high-sensitivity model that incorporates a temperature-controlled cell with a cooling function as standard. RF-20A Standard model The RF-20A, which offers best-in-class sensitivity, features a water Raman S/N ratio of at least 1200, as well as excellent ease-of-use with such features as maintenance from the front panel and adoption of a long-life lamp.
Support for Ultra Fast Analysis Switch from Conventional LC to Ultra Fast LC Fast response is required to follow the sharp peaks obtained in ultra fast LC analysis. The 10 ms response of the RF-20A/20Axs permits ultra fast LC analysis with no loss of separation. In this analysis example, the analysis time was reduced by a factor of more than three, while maintaining the separation. Conventional LC (N for Peak δ = 11,708 ) α β γ Analysis Conditions Mobile phase Hexane / 2-propanol = 100 / 0.
Refractive Index Detector RID-20A The RID-20A incorporates an auto purge function of the reference cell and a validation support function, inheriting the stability and expandability of Prominence HPLC series. Excellent Reproducibility in Molecular Weight Distribution Analysis The RID-20A differential refractive index detector allows productivity improvements in GPC analysis.
LabSolutions GPC Software Easy Analysis of Molecular Weight Distribution via Graphical Interface Create a Calibration Curve in Just Three Steps Drag and drop standard-sample data into the window. Load the retention times. Enter the molecular weight of the standard sample. A maximum of 64 data points are available. Virtual points are also easy to set, and calibration curve appropriateness can be checked visually while choosing from a wide variety of approximation equations.
Chromatography Workstation LabSolutions Flexible Instrument Access With LabSolutions, LCs and GCs can be operated via the same interface, and up to four LC and/or GC systems can be controlled simultaneously on a single PC. Instrument information for up to 16 systems can be registered to a single PC, allowing the system to be switched between LC or GC systems connected to the network.
LabSolutions CS Freely Accessible to the Analysis Network Since all analytical data are managed in the database of a server computer, LabSolutions CS can read data from any personal computer on a network. In addition, analysis directions and instrument monitoring and control can be performed from a personal computer (client PC) not connected to the instruments. Furthermore, it allows direct control of another vendor’s LCs and GCs.
Solvent Delivery Unit / Autosamplers LC-10Ai LC-10Ai Solvent Delivery Unit for Bio-inert HPLC System This bio-inert solvent delivery unit incorporates a serial dual plunger and offers low-pulsation performance from an optimized cylinder volume. It can be used together with the SIL-10Ai and SPD-20A (with bio-inert cell) to construct a high-performance bio-inert LC system. The LC-10Ai uses PEEK resin in liquid contact parts and is ideal for the analysis of physiologically active substances and metal ions.
Column Oven / Fraction Collector CTO-10ASVP The CTO-10ASVP is a space-saving, compact and affordable oven with a block heating mechanism. The CTO-10ASVP controls temperature from −15°C below room temperature to 60°C, and accommodates two manual injectors. Specifications CTO-10ASVP (228-45059-XX) Type Block heating Cooling method Electronic cooling Temperature setting range 4 to 80°C Temperature control precision ±0.
Conductivity Detector CDD-10AVP Handles a Wide Variety of Analysis Options The CDD-10AVP conductivity detector achieves an even higher level of sensitivity and makes it possible to perform a wide variety of analysis scenarios with a single unit. An option card enables the simultaneous 2-channel measurement of anions and cations, and a suppressor option allows expansion to a suppressor system for ultra-high sensitivity work.
Evaporative Light-Scattering Detector ELSD-LT ll Highly Versatile Detection Method Not all compounds have a chromophore or other such structural property that allows the use of an absorbance detector. Refractive Index Detection (RID) is one option but it suffers from the inability to run gradient analysis. Evaporative Light Scattering Detection (ELSD) is a perfect alternative to RID as it is more rugged, quicker to stabilize, and gradient compatible.
FCV Series Flow-Line Selection Valves FCV-20AH 2 (228-45015-XX) FCV-20AH 6 (228-45017-XX) High-Pressure Flow-Line Selection Valves The FCV-20AH2/20AH6 is a stand-alone, high-pressure, flow-line selection valve. The valve position is controlled by event signal input. Direct control is also possible from the unit itself. FCV-11AL FCV-11ALS (228-45048-58) Reservoir Selection Valves (228-45049-58) These solenoid valve units can automatically switch between two solvents (e.g.
FCV-14AH/14AHi FCV-34AH (228-45014-57/58) Column Switching Valves (228-45185-41) This unit performs automatic column selection and incorporates a 7-port, 6-position, high-pressure valve. It can be used for automatic multi-column switching. (Two units used.) Column 1 2 Injector 2 1 6 FCV-14AH 3 3 2 3 1 4 4 4 5 5 5 Detector 6 FCV-14AH * The liquid contact parts of the FCV-14AHi have bio-inert specifications.
Optional Accessories Options for Solvent Delivery Units Automatic Rinsing Kit • 20AD Automatic Rinsing Kit (228-45567-91) • 20AT Automatic Rinsing Kit (228-45568-91) • 20AB Automatic Rinsing Kit (228-18803-92) * The automatic rinse kit for the LC-20AB is shown in the photograph. These optional kits are used to continuously, automatically rinse the backs of the plunger seals and plunger units.
Valve Options Option Box VP (228-45060-91) Sub-controller VP (228-35308-91) * Option Box VP is shown in the photograph. Option Box VP can house up to two FCV-11AL(S)/12AH/13AL/14AH units. One FCV-11AL or FCV-11ALS unit, up to two FCV-12AH units, up to two FCV-13AL/14AH units, and one DGU-10B unit can be controlled from the CBM-20A or SCL-10AVP via Option Box VP. Sub-controller VP has the same control functions as Option Box VP but has no housing capability.
From HPLC to UHPLC —Extensive Lineup of Shimadzu LCs— Shimadzu’s extensive LC lineup fulfills a wide range of analytical needs, from conventional to ultra-high speed analysis. With scalable column size and packing material particle size, Shimadzu can provide an LC system most appropriate for your applications.
The flagship UHPLC system supporting columns with sub-2 µm micro-particle packing materials, realizing both ultra-high speed and ultra-high separation. Excellent reproducibility of low injection volume and ultra-low carryover ensures reliable data, even with ultra-sensitive LC/MS/MS methods. The UHPLC system supporting most commercial UHPLC and HPLC columns. Superior gradient performance and minimized delay volume enable ultra-high speed analyses with excellent reproducibility.
Specifications Solvent Delivery Units LC-20AD / 20AT / 20AB P. 6 LC-20AD (228-45000-XX) LC-20AT (228-45001-XX) LC-20AB (228-45002-XX) Solvent delivery method Parallel-type double plunger Serial-type double plunger Parallel-type double plunger (2 sets) Plunger capacity 10 µL Primary side: 47 µL, Secondary side: 23 µL 10 µL Flow rate setting range 0.0001 to 10.0000 mL/min 0.001 to 10.000 mL/min 0.0001 to 10.
Autosamplers SIL-20A / 20AC / 20AHT / 20ACHT P. 8 SIL-20A (228-45006-XX) Maximum operating pressure SIL-20AHT (228-45119-XX) SIL-20AC (228-45007-XX) SIL-20ACHT (228-45120-XX) Total-volume sample injection, variable injection volume Injection method 20 MPa 35 MPa 20 MPa 35 MPa Injection-volume setting range 0.1 to 100 µL (standard), 0.1 to 2,000 µL (option) Number of processed samples 175 (1 mL vials), 105 (1.5 mL vials), 50 (4 mL vials) 175 (1 mL vials), 70 (1.
Specifications Column Oven CTO-30A P. 12 CTO-30A (228-45160-XX) Type Block heating system Te Tempe Temperature setting range 4 to 150°C in 1°C steps Tem mpera Temperature control precision ± 0.05°C (room temperature 25°C) Te empe Temperature control range 5°C above room temperature to 150°C C Colum Column size and capacity 150 mmL. × 4.6 mmI.D. column × 2 Device es tha Devices that can be accommodated Gradient Mixer, High-Pressure Flow Switching Valves (2), Post Column Cooler etc.
UV-VIS Detectors SPD-20A / 20AV Photodiode Array Detector SPD-M20A Light source Number of diode elements Wavelength range Bandwidth, slit width SPD-20A Wavelength accuracy Wavelength precision Noise Drift Linearity Functions Cell Cell temperature control range Web control Buffer memory SPD-M20A Op Operating temperature range Dimensions, weight Power requirements P.
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