INSTRUCTION MANUAL Linear Gradient Makers GM-20 GM-40 GM-100 GM-150 GM-200 GM-500 GM-1000 GM-2000
TABLE OF CONTENTS Page Important User Information Section 1 1.1 1.2 1.3 Section 2 2.1 2.2 Section 3 3.1 3.2 Section 4 4.1 4.2 Section 5 3-4 General Information Introduction Specifications Safety 5 5 5 Description of parts Unpacking Components/Assembly 6 6 Instructions for Use Gradient Maker Preparation General Notes 6-7 8 Maintenance of Equipment Care and Handling Maintenance 9 9 Accessories 9 C.B.S.
ENGLISH IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d'utilisation explique la manière de se servir efficacement du produit en conditions de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction The C.B.S. Scientific line of linear gradient makers can be used to make reproducible gradients easily up to 2000mls total volume by filling each cylindrical chamber. Eight different sizes of linear gradient makers with side outlets are available. Gradient formation is controlled by a Teflon valve centered between the two chambers. Gradient flow rate is controlled either by the valve fitted on the outflow male luer adapter, or by a peristaltic pump. 1.
Be sure that all gradient makers are secure either by attaching support rod to a ring stand or mounting on a level surface. SECTION 2 Description of Parts 2.1 Unpacking Remove the gradient maker from the shipping container and place on a level surface in an authorized area. Verify that the catalog number on the gradient maker matches the packing list. Check for any damage which may have occurred during shipping. Contact C.B.S. Scientific customer service immediately if any damage is found. 2.
2. When using the 10ml, 20ml or 50ml chambers attach gradient maker to a ring stand support and drop a small magnetic flea or spin-bar into chamber on the right hand side (‘B’). Ensure both valves are closed. Set up magnetic stirrer. 3. Outlet # 1 should be fitted with luer type valve and needle with attached tubing to deliver acrylamide mix to gel plate sandwich. 4. Pour solution ‘B’ into right side of gradient maker. Turn on magnetic stirrer so that it gently mixes the solution. 5.
3.2 General Notes In general, a gradient maker consists of two cylindrical chambers, with connecting channel between them. The two chambers may be arranged side by side or in a concentric arrangement. Each chamber differs according to which flow outlet or “valve” is connected to it. One flow valve is connected between the two chambers. The second flow valve is to be connected to tubing. The gradient gel should be separated into two solutions; each one-half the final volume of the total gel volume.
SECTION 4 Maintenance of Equipment 4.1 Care and Handling Do not use organic solvents to clean the gradient maker. Use only deionized water. After each use, immediately pump deionized water through both chambers to clear residual acrylamide. Allow to air dry; do not use drying oven. 4.2 Maintenance Replacement parts can be ordered by calling 1-858-755-4959 or by contacting your local distributor. SECTION 5 Gradient Makers, Linear Cat.
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CONTACT US Telephone: Local or International 858-755-4959 Toll Free: 858-755-0733 Fax: 858-755-0733 Check out our new and improved online ordering! www.cbsscientific.com E-mail address: sales@cbssci.com Mailing address: C.B.S. Scientific Company P.O. Box 856 Del Mar, CA 92014 Shipping address: C.B.S.
