INSTRUCTION MANUAL ECONO-SUBMARINE PRODUCTIVITY PACKAGES GCSGE-014-02 GCSGE-020-02 GCSGE-030-02 GCSGE-040-02
TABLE OF CONTENTS Page Important User Information 3-4 Section 1 1.1 1.2 1.3 General Information Introduction Specifications Safety 5 5 5 Section 2 2.1 2.2 Description of parts Unpacking Components/Assembly 6 6 Section 3 3.1 3.2 3.3 3.4 3.5 Instructions for Use Econo-Sub Unit Preparation Gel Casting Running the Gel Removing the Gel Maximum Well/Comb volumes 7 8-9 10 10-11 11 Section 4 4.1 4.2 Running Conditions Recommended Power Recommended Buffers 12 12 Section 5 5.1 5.
ENGLISH IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d'utilisation explique la manière de se servir efficacement du produit en conditions de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction The Econo-Sub Productivity Packages offer gel sizes (14 x 20cm) GCSGE-014-02, (20 X 20cm) GCSGE-020-02, (20 x 30cm) GCSGE-030-02, and (20 x 40cm) GCSGE-040-02. The gel tray and safety cover are UV transparent acrylic. In the Econo-Sub Horizontal gel unit, gel casting is done in the gel tray by sealing the ends of the tray with tape. The slotted gel tray configuration allows for single or multiple sample separations.
SECTION 2 Description of Parts 2.1 Unpacking Please verify that your unit comes complete with the following components: • Econo-Sub Horizontal electrophoresis unit • UV Transparent safety cover with attached DC power leads • 2 UV Transparent slotted gel trays • 2 Adjustable height combs (of your choice) White tubing adopters for buffer and coolant circulation • Bubble level • External Gel caster (not shown below) • 2.2 Components/Assembly C.B.S.
SECTION 3 Instructions for Use 3.1 Econo-Sub Unit Preparation Place Econo-Sub unit on a level work surface in an authorized work area. Level Econo-Sub unit by placing the bubble level in the center of the unit and adjusting the four leveling screws on the of base until the unit is level. Place the adjustable height comb(s) assembly in the gel tray. Loosen the white screws holding the comb to the backing and adjust comb depth (generally 2mm). Gently retighten the adjusting screws and set the comb aside.
3.2 Gel Casting Gel Casting into Gel Tray. 1. Tape both ends of gel tray with tape. 2.Position the gel comb(s) in the gel tray. Using a pipette or a pipetor, run a bead of agarose solution along the edges of the gel tray where it meets the tape to assure a leak-proof seal. Then pour the agarose solution into the gel tray. Allow the agarose slab to cool uncovered until it turns opaque. NOTE: (Optional storage) Precast gels can be stored for up to one month if wrapped in plastic wrap and stored at 4°C.
B. Gel Casting in External Gel Caster. 1. Level external gel caster with leveling screws on base. Push one of the open sides of gel tray against black gasket. 2. Push down on gel tray until flat against bottom of tray and so both sides friction fit against the black gaskets. 3. Place gel comb(s) into desired slot(s) in gel tray and pour in hot agaorse. When agarose has cooled place gel tray into Hoizontal Unit. Remember, current travels from negative (black cathode) towards positive (red anode). C.B.S.
3.3 Running the Gel 1. Add enough buffer to fill both reservoirs and overflow the surface of the gel to a depth of 2-3mm. Gently remove comb(s). Flush out any air bubbles in the wells. Load the samples into the sample wells. 2. Place the safety cover on the unit. 3. Connect the leads to the power supply, matching the color-coded red to red and black to black. See Section 4.1 for recommended power conditions. Begin separation by electrophoresis. 3.4 Removing the Gel 1.
3. Gently lift the gel tray from the unit. Always wear gloves, eye protection and protective clothing if buffer and/or gel contains Ethidium Bromide. Ethidium Bromide is a powerful mutagen, gloves, eye protection and protective clothing should always be worn when handling the gel or buffer solutions. See Material Data Safety Sheets. 4. View separated fragments under UV light, using proper protection for eyes and skin (see manufacturer’s instructions). 3.
SECTION 4 Running Conditions 4.1 Recommended Power The recommended power conditions for optimal resolution are 50 to 150VDC, constant, and 25 to 80 mA. The usual run time will vary for the voltage chosen but should range from 15 to 60 minutes, monitoring nucleic acid migration by progress of marker dyes. Constant power is not a necessity, but it produces uniform heat throughout the run. Be sure the polarity is correct i.e.
SECTION 5 Maintenance of Equipment 5.1 Care and Handling The plastic components of the Econo-Sub units are fabricated from acrylic and polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and reliable performance. After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe dry with a soft cloth or paper towel, or allow to air dry.
SECTION 6 Econo-Submarine Gel Kits and Accessories Cat.# Item GCSGE-014-02 Econo-Submarine Gel Kit-CE. Gel bed dimensions are 14cm x 20cm. Includes 2 gel trays and 2 combs, & external gel caster, please specify comb cat.#’s from list below. GCSGE-020-02 Econo-Submarine Gel Kit-CE. Gel bed dimensions are 20cm x 20cm. Includes 2 gel trays and 2 combs, & external gel caster, please specify comb cat.#’s from list below. GCSGE-030-02 Econo-Submarine Gel Kit-CE. Gel bed dimensions are 20cm x 30cm.
GEL TRAYS (UV TRANSPARENT) Cat. # Item GEL SEALING TAPE Cat. # Item SGT-014 SGT-020 SGT-030 SGT-040 GT-72-10 GT-72-15 Gel Tray for 14cm x 20cm Gel Tray for 20cm x 20cm Gel Tray for 20cm x 30cm Gel Tray for 20cm x 40cm GEL COMB BRIDGE Cat. # Item GCB-1400 GCB-2000 Bridge for 14cm wide units Bridge for 20cm wide units GEL SCOOP Cat. # Item GS-1400 GS-2000 GS-3000 GS-4000 14cm x 20cm 20cm x 20cm 20cm x 30cm 20cm x 40cm Gel tape, 72 yard x 1.0” Gel tape, 72 yard x 1.5” GEL TRAY DIVIDER BAR Cat.
CONTACT INFORMATION Telephone: Local or International 858-755-4959 Toll Free: 800-243-4959 Fax: 858-755-0733 Online ordering: www.cbsscientific.com E-mail address: sales@cbssci.com Mailing address: C.B.S. Scientific Company P.O. Box 856 Del Mar, CA 92014 Shipping address: 10805 Vista Sorrento Parkway Ste 100 San Diego, CA 92121 Credit Card Options: Visa/Mastercard Discover/American Express www.cbsscientific.
